##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1100987
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.179550712224575	32.0	32.0	32.0	32.0	32.0
2	31.19581520944389	32.0	32.0	32.0	32.0	32.0
3	31.342205675453027	32.0	32.0	32.0	32.0	32.0
4	31.47188840558517	32.0	32.0	32.0	32.0	32.0
5	31.383102616107184	32.0	32.0	32.0	32.0	32.0
6	34.95678059777273	36.0	36.0	36.0	36.0	36.0
7	34.991629328956655	36.0	36.0	36.0	36.0	36.0
8	34.919264260159295	36.0	36.0	36.0	36.0	36.0
9	35.08363404835843	36.0	36.0	36.0	36.0	36.0
10	34.8705606878192	36.0	36.0	36.0	32.0	36.0
11	35.08239606825512	36.0	36.0	36.0	36.0	36.0
12	34.96422482735945	36.0	36.0	36.0	36.0	36.0
13	35.020836758290514	36.0	36.0	36.0	36.0	36.0
14	34.953835058906236	36.0	36.0	36.0	32.0	36.0
15	34.90359831678303	36.0	36.0	36.0	32.0	36.0
16	34.92968672654627	36.0	36.0	36.0	32.0	36.0
17	34.88659902432999	36.0	36.0	36.0	32.0	36.0
18	34.87567609790125	36.0	36.0	36.0	32.0	36.0
19	34.884309260690635	36.0	36.0	36.0	32.0	36.0
20	34.88177153772025	36.0	36.0	36.0	32.0	36.0
21	34.86474681354094	36.0	36.0	36.0	32.0	36.0
22	34.84923073569443	36.0	36.0	36.0	32.0	36.0
23	34.79533091671382	36.0	36.0	36.0	32.0	36.0
24	34.7715722347312	36.0	36.0	36.0	32.0	36.0
25	34.74186071225183	36.0	36.0	36.0	32.0	36.0
26	34.67440669145049	36.0	36.0	36.0	32.0	36.0
27	34.66390792988473	36.0	36.0	36.0	32.0	36.0
28	34.64327825850805	36.0	36.0	36.0	32.0	36.0
29	34.60386907384011	36.0	36.0	36.0	32.0	36.0
30	34.57849366068809	36.0	36.0	36.0	32.0	36.0
31	34.574983174188255	36.0	36.0	36.0	32.0	36.0
32	34.525362243150916	36.0	36.0	36.0	32.0	36.0
33	34.494427272983245	36.0	36.0	36.0	32.0	36.0
34	34.46500458225211	36.0	36.0	36.0	32.0	36.0
35	34.42931115444596	36.0	36.0	36.0	32.0	36.0
36	34.39486842260626	36.0	36.0	36.0	32.0	36.0
37	34.38202176774113	36.0	36.0	36.0	32.0	36.0
38	34.33857892963314	36.0	36.0	36.0	32.0	36.0
39	34.324961148496755	36.0	36.0	36.0	32.0	36.0
40	34.30703995596678	36.0	36.0	36.0	32.0	36.0
41	34.28798977644605	36.0	36.0	36.0	32.0	36.0
42	34.20904878985856	36.0	36.0	36.0	32.0	36.0
43	34.20198331133792	36.0	36.0	36.0	32.0	36.0
44	34.12374533032634	36.0	36.0	36.0	32.0	36.0
45	34.08656323825804	36.0	36.0	36.0	32.0	36.0
46	34.053151399607806	36.0	36.0	36.0	32.0	36.0
47	34.011386147157054	36.0	36.0	36.0	32.0	36.0
48	33.95085409727817	36.0	36.0	36.0	32.0	36.0
49	33.922982741848905	36.0	36.0	36.0	32.0	36.0
50	33.25627550552368	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	8.0
21	33.0
22	112.0
23	315.0
24	925.0
25	2125.0
26	4343.0
27	8178.0
28	13627.0
29	21217.0
30	31546.0
31	45503.0
32	67307.0
33	110662.0
34	225646.0
35	569439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14561633146909	17.858683813958304	11.53300093651245	26.462698918060156
2	15.95083666141984	20.170701999940054	37.0900368125575	26.7884245260826
3	18.353251915108512	24.712938086181246	28.487295886762908	28.446514111947334
4	11.98533679325914	15.695825654617176	35.86418368245947	36.45465386966422
5	13.974279441991595	37.34639918545814	33.24462505006871	15.434696322481557
6	34.69154012857566	35.636602100299186	16.479319446387148	13.192538324738006
7	30.314127803739378	30.332838322047984	20.779483825846857	18.57355004836578
8	28.61841238815717	33.174596975259476	18.839550330748683	19.367440305834673
9	27.713713757354796	13.334277944598549	17.94979391125377	41.00221438679288
10	15.474346875547809	26.86777289625487	32.01883418091386	25.639046047283458
11	37.87810391948315	20.381712045646317	22.502627188150267	19.23755684672026
12	25.00803824935104	23.29194013366201	29.134884548940676	22.56513706804628
13	30.18846845537612	19.291721920470852	25.13269995821904	25.387109665933988
14	23.508270306552213	19.412127481977535	25.16669134149631	31.912910869973942
15	24.99607154243506	27.52427241786799	22.289841144984155	25.189814894712796
16	25.523402090883497	25.331389592813608	24.26705541477061	24.878152901532285
17	23.857229921879185	25.971787132818097	25.19675527503958	24.974227670263137
18	24.7849879880289	24.618319050668266	26.75822104751654	23.83847191378629
19	25.90711788604225	24.44960748855345	25.269326522474834	24.373948102929464
20	26.132579824193314	24.02191861447326	25.101954437038938	24.743547124294494
21	26.811215754591107	23.899737235771177	24.4367099702358	24.852337039401917
22	26.05582632295052	24.27151662237304	24.8563560299586	24.816301024717845
23	24.44497124831627	24.255140197951096	25.562703739126725	25.73718481460591
24	25.17459397647375	24.68866458428513	25.217373928571103	24.91936751067001
25	24.94002988287548	24.412220188652682	25.45775751272747	25.18999241574437
26	24.539203575191657	25.27840351705846	25.752461577589653	24.42993133016023
27	25.14169966936744	24.845947026123604	24.976383388438762	25.035969916070194
28	24.394745590594237	24.73917790401311	25.621967369761535	25.24410913563112
29	24.58107053746681	24.759005995671053	25.441318993134182	25.21860447372795
30	24.38888364082926	25.010899816156435	25.771525320272932	24.828691222741377
31	25.136501828956465	24.71239466582372	24.765532958912743	25.38557054630707
32	24.759730014741827	25.079000026340914	24.688155400477225	25.473114558440034
33	24.295373581436923	24.7391769563772	25.513607487433443	25.451841974752437
34	25.114514009175654	24.772084649990873	25.47792511424152	24.63547622659196
35	25.396836930812857	24.83977561319435	25.453969095949336	24.309418360043452
36	24.407212280861113	25.28367699155237	25.20374239258788	25.10536833499864
37	25.237501373824756	25.056201921810523	24.837207443450648	24.869089260914073
38	24.616564068885395	25.042669595177042	25.143405265323587	25.197361070613976
39	25.352472164645057	24.69085452586794	24.72573387420545	25.230939435281552
40	25.70549344349104	24.92354448407627	25.080588246514203	24.290373825918486
41	24.685452900591127	25.042781312105568	25.63935808049063	24.63240770681267
42	25.615125050524316	25.628840415820807	24.85015282186829	23.905881711786588
43	24.77676604827539	24.86123663116783	25.227184995117963	25.134812325438816
44	24.42762097231999	25.371965757623926	24.877949090578806	25.322464179477283
45	24.61672608369854	25.614478076250595	24.792570221848816	24.976225618202047
46	24.474169666286095	25.08072405045355	24.960285129608085	25.48482115365227
47	25.198735648888242	24.83405391658189	25.170941723586687	24.796268710943174
48	25.332913095172138	25.556263017013286	24.193910240398633	24.916913647415946
49	24.716367101036074	25.4215551012141	24.590297942838355	25.271779854911475
50	24.722858780369727	25.88047118321703	24.25590818303446	25.140761853378784
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	24.5
2	18.0
3	59.0
4	100.0
5	142.0
6	184.0
7	181.5
8	179.0
9	231.5
10	284.0
11	472.5
12	661.0
13	1177.0
14	1693.0
15	2308.0
16	2923.0
17	3247.5
18	3572.0
19	3793.0
20	4014.0
21	4498.5
22	4983.0
23	5575.0
24	6167.0
25	6461.0
26	6755.0
27	8421.0
28	10087.0
29	11667.5
30	13248.0
31	15693.5
32	18139.0
33	21763.5
34	25388.0
35	29549.0
36	33710.0
37	37681.0
38	41652.0
39	43925.0
40	46198.0
41	49916.5
42	53635.0
43	54605.5
44	55576.0
45	61702.0
46	67828.0
47	72346.0
48	76864.0
49	79948.5
50	83033.0
51	81506.0
52	79979.0
53	79351.0
54	78723.0
55	78781.0
56	78839.0
57	77520.5
58	76202.0
59	69885.5
60	63569.0
61	56360.5
62	49152.0
63	42831.0
64	36510.0
65	31271.0
66	26032.0
67	22112.5
68	18193.0
69	16340.0
70	14487.0
71	11144.5
72	7802.0
73	6639.5
74	5477.0
75	4363.0
76	3249.0
77	2770.0
78	2291.0
79	1912.5
80	1534.0
81	1205.5
82	877.0
83	732.0
84	587.0
85	439.0
86	291.0
87	203.0
88	115.0
89	80.5
90	46.0
91	43.0
92	40.0
93	31.5
94	23.0
95	21.5
96	20.0
97	15.5
98	11.0
99	13.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008537793815912449
2	3.633103751452106E-4
3	4.541379689315132E-4
4	0.0
5	0.0
6	9.082759378630266E-5
7	1.816551875726053E-4
8	0.0
9	4.541379689315132E-4
10	9.082759378630264E-4
11	0.0
12	9.082759378630266E-5
13	6.357931565041186E-4
14	0.0
15	0.004178069314169922
16	0.001544069094367145
17	0.0
18	1.816551875726053E-4
19	0.0
20	4.541379689315132E-4
21	0.0
22	9.082759378630266E-5
23	7.266207502904212E-4
24	3.633103751452106E-4
25	0.0019982070632986585
26	0.006085448783682278
27	0.006085448783682278
28	0.004087241720383619
29	0.002361517438443869
30	0.004632207283101436
31	0.007811173065622029
32	0.003451448563879501
33	0.0044505520955288305
34	0.0027248278135890793
35	0.0028156554073753824
36	0.007902000659408331
37	0.004178069314169922
38	0.007811173065622029
39	0.0044505520955288305
40	0.0018165518757260529
41	0.0035422761576658036
42	0.002906483001161685
43	0.0010899311254356319
44	0.0010899311254356319
45	0.0010899311254356319
46	0.0012715863130082373
47	0.002452345032230172
48	0.0021798622508712637
49	3.633103751452106E-4
50	0.0012715863130082373
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1100987.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.15868938502808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.89227660296406	38.748578905045164
2	14.981281483227507	15.927705779131276
3	5.48246229695516	8.743215309269008
4	2.49344041684327	5.3019209847618525
5	1.2895417059914218	3.42751734989186
6	0.7613283733255661	2.428273111057348
7	0.4834136124145624	1.7988343846788053
8	0.29978102878725116	1.2748773274260516
9	0.21180247055715612	1.0133227568996475
>10	0.9574293228638431	8.774449961553703
>50	0.07080344198473104	2.6073207556355644
>100	0.07008362327155199	7.455306477646187
>500	0.005324979600883348	1.9146417620366527
>1k	0.0010306412130741962	0.5840351349669225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1131	0.1027260085723083	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1106	0.10045531872765073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.082759378630266E-5
2	0.0	0.0	0.0	0.0	9.082759378630266E-5
3	0.0	0.0	0.0	0.0	9.082759378630266E-5
4	0.0	0.0	0.0	0.0	9.082759378630266E-5
5	0.0	0.0	0.0	0.0	1.816551875726053E-4
6	0.0	0.0	0.0	0.0	1.816551875726053E-4
7	0.0	0.0	0.0	9.082759378630266E-5	1.816551875726053E-4
8	0.0	0.0	0.0	9.082759378630266E-5	1.816551875726053E-4
9	0.0	0.0	0.0	9.082759378630266E-5	1.816551875726053E-4
10	0.0	0.0	0.0	1.816551875726053E-4	1.816551875726053E-4
11	0.0	0.0	0.0	1.816551875726053E-4	1.816551875726053E-4
12	0.0	0.0	0.0	1.816551875726053E-4	9.082759378630266E-4
13	0.0	0.0	0.0	1.816551875726053E-4	9.991035316493293E-4
14	0.0	0.0	0.0	1.816551875726053E-4	9.991035316493293E-4
15	0.0	0.0	0.0	1.816551875726053E-4	0.0011807587192219345
16	0.0	0.0	0.0	1.816551875726053E-4	0.0013624139067945399
17	0.0	0.0	0.0	1.816551875726053E-4	0.0013624139067945399
18	0.0	0.0	0.0	1.816551875726053E-4	0.0016348966881534477
19	0.0	0.0	0.0	1.816551875726053E-4	0.0016348966881534477
20	0.0	0.0	0.0	1.816551875726053E-4	0.002089034657084961
21	0.0	0.0	0.0	2.7248278135890797E-4	0.002361517438443869
22	0.0	0.0	0.0	6.357931565041185E-4	0.002543172626016474
23	0.0	0.0	0.0	0.0017257242819397505	0.002543172626016474
24	0.0	0.0	0.0	0.0032697933763068954	0.002543172626016474
25	0.0	0.0	0.0	0.004178069314169922	0.002543172626016474
26	0.0	0.0	0.0	0.004723034876887738	0.002543172626016474
27	0.0	0.0	0.0	0.005722138408537067	0.002634000219802777
28	0.0	0.0	0.0	0.007811173065622028	0.002634000219802777
29	0.0	0.0	0.0	0.01235255275493716	0.002634000219802777
30	0.0	0.0	0.0	0.019618760257841372	0.002634000219802777
31	0.0	0.0	0.0	0.04078158961004989	0.002634000219802777
32	0.0	0.0	0.0	0.06130862580575429	0.0027248278135890798
33	0.0	0.0	0.0	0.078929179000297	0.0027248278135890798
34	0.0	0.0	0.0	0.0990929048208562	0.0029973105949479876
35	0.0	0.0	0.0	0.12752194167596892	0.0029973105949479876
36	0.0	0.0	0.0	0.16185477212719132	0.0029973105949479876
37	0.0	0.0	0.0	0.2152613972735373	0.0029973105949479876
38	0.0	0.0	0.0	0.2916474036478178	0.0029973105949479876
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGA	50	8.3306964E-5	26.404688	33
TAGACAG	225	0.0	21.509068	5
GTATATG	125	9.968062E-10	21.117992	1
CGTATAT	225	0.0	20.53325	10
CGCAATC	75	5.472241E-5	20.533249	44
ATTAGAC	75	5.4760698E-5	20.531382	3
TGTAGGA	785	0.0	20.456656	2
GTAGGAC	750	0.0	20.238077	3
ATAGCGT	230	0.0	20.085049	6
GTATAGA	100	1.1242737E-6	19.79812	1
TAGCGTA	245	0.0	19.753227	7
GTCCTAC	595	0.0	19.594778	1
TCCTACA	630	0.0	19.204525	2
GGCGTTA	230	0.0	19.130354	42
TATGGCG	785	0.0	19.055515	16
GATATAC	220	0.0	18.998196	1
AATCGTA	70	8.12292E-4	18.857065	13
AGCGTAT	245	0.0	18.855352	8
AATCACG	355	0.0	18.594006	34
GCGAGAA	510	0.0	18.548944	20
>>END_MODULE