FastQCFastQC Report
Thu 2 Feb 2017
SRR4063956_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063956_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118132
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25250.2258230691903997No Hit
TATCAACGCAGAGTACTTTTTTTTT18610.16643830960924114No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12210.10919998712137745No Hit
GTATCTGATCGTCTTCGAACCTCCG11200.10016706435376145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCG250.006035554718.997544
GGACCGA551.1272139E-515.5462236
GCTCGTA704.4280023E-714.9299779
ATCCCGA400.00527614314.25070516
TCCAACG1257.2759576E-1213.67945218
GGCGAGG2600.013.15331819
AAGCTCG801.9958552E-613.063737
CGACCCG957.383278E-812.9994795
TCGCGTA905.394231E-712.667869
CCATTAA905.39716E-712.6672931
ACAATAC905.411839E-712.6644593
CGGTCGG1001.439239E-712.35116310
TGATACT853.9462266E-612.2925254
GCGTTAT951.0382737E-612.0005931
CGCATCG1603.6379788E-1211.87452413
CCAACGA802.8658462E-511.87452419
TTACCCT802.868472E-511.8734624
ATAAGGG658.0320647E-411.6902693
AACAATA907.46968E-611.6090882
GTATCAA17550.011.5304851
Produced by FastQC (version 0.11.5)