##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063956_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1118132
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257056411944205	32.0	32.0	32.0	32.0	32.0
2	30.9578189337216	32.0	32.0	32.0	32.0	32.0
3	30.97852131948643	32.0	32.0	32.0	32.0	32.0
4	30.99424754858997	32.0	32.0	32.0	32.0	32.0
5	30.94554310224553	32.0	32.0	32.0	32.0	32.0
6	34.570038242354215	36.0	36.0	36.0	32.0	36.0
7	34.51206744820826	36.0	36.0	36.0	32.0	36.0
8	34.48295728947924	36.0	36.0	36.0	32.0	36.0
9	34.57537303287984	36.0	36.0	36.0	32.0	36.0
10	34.36320398664916	36.0	36.0	36.0	32.0	36.0
11	34.54781456929951	36.0	36.0	36.0	32.0	36.0
12	34.406038821892224	36.0	36.0	36.0	32.0	36.0
13	34.47303359531791	36.0	36.0	36.0	32.0	36.0
14	34.395204680663824	36.0	36.0	36.0	32.0	36.0
15	34.345460106677926	36.0	36.0	36.0	32.0	36.0
16	34.34570068650213	36.0	36.0	36.0	32.0	36.0
17	34.27714527443987	36.0	36.0	36.0	32.0	36.0
18	34.28359889530038	36.0	36.0	36.0	32.0	36.0
19	34.2844726740671	36.0	36.0	36.0	32.0	36.0
20	34.245790300250775	36.0	36.0	36.0	32.0	36.0
21	34.21681339949129	36.0	36.0	36.0	32.0	36.0
22	34.219527748065524	36.0	36.0	36.0	32.0	36.0
23	34.1790763523448	36.0	36.0	36.0	32.0	36.0
24	34.15242207539003	36.0	36.0	36.0	32.0	36.0
25	33.76585680402672	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	10.0
5	9.0
6	49.0
7	23.0
8	64.0
9	96.0
10	112.0
11	26.0
12	56.0
13	66.0
14	96.0
15	209.0
16	304.0
17	453.0
18	520.0
19	748.0
20	1079.0
21	1513.0
22	2174.0
23	3391.0
24	4978.0
25	7515.0
26	10508.0
27	14414.0
28	19978.0
29	27693.0
30	37214.0
31	51294.0
32	73417.0
33	105119.0
34	232525.0
35	522477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.957350143955566	17.571078619959092	11.572452863275316	25.899118372810026
2	16.19105967372639	19.942580137378364	38.55743775042931	25.30892243846594
3	18.92409897380768	24.012399451218748	28.68392668368342	28.379574891290154
4	12.40297112499385	15.749556164726611	36.32929223366529	35.51818047661424
5	14.338191887859697	36.84603605634206	33.87097639772886	14.944795658069388
6	33.532084119347076	35.875500790262464	17.264931577671575	13.327483512718889
7	29.778318887667314	30.514005691195823	20.972351809293947	18.73532361184291
8	27.46450267963389	33.72088861848992	19.587542162853744	19.22706653902245
9	27.36164512754803	14.230045482243314	18.7991184515815	39.60919093862716
10	15.964756226990517	26.855926849304772	31.843872877579255	25.33544404612546
11	36.752101569756725	20.99797941472826	23.19547775477003	19.054441260744987
12	24.86649752325722	23.608213747152977	29.434980467963435	22.090308261626372
13	29.643163018468748	19.787435307467955	25.352402584211042	25.216999089852255
14	23.18347957141681	20.38553513830865	25.554075871969182	30.876909418305353
15	24.582681914709877	28.253506026759545	22.486180379494787	24.67763167903579
16	24.872847946459657	25.977412890886846	24.931199619103193	24.218539543550303
17	23.412433484099722	26.24847637645181	25.856048226153167	24.483041913295306
18	23.95915378997265	25.31514485385728	27.348057397356403	23.377643958813675
19	25.471986882640085	24.860780203669925	25.641771488000515	24.025461425689475
20	25.519290755384898	24.887647740647754	25.368741860558785	24.224319643408563
21	26.435076953920728	24.384583439244174	24.662666518689832	24.517673088145266
22	25.624233050585772	25.06357121184049	25.215193362995425	24.09700237457832
23	24.18296324408705	24.849446119254427	25.88604807699193	25.081542559666598
24	24.739226711204434	25.154351651459738	25.838081582164847	24.26834005517098
25	24.47301645550926	25.05014522199498	25.903218155210762	24.573620167285
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	112.0
1	112.0
2	283.5
3	455.0
4	455.0
5	455.0
6	1140.0
7	1825.0
8	1825.0
9	1825.0
10	2004.0
11	2183.0
12	2183.0
13	2183.0
14	2543.0
15	2903.0
16	2903.0
17	2903.0
18	5193.0
19	7483.0
20	7483.0
21	7483.0
22	12575.0
23	17667.0
24	17667.0
25	17667.0
26	26942.5
27	36218.0
28	36218.0
29	36218.0
30	46363.0
31	56508.0
32	56508.0
33	56508.0
34	67017.0
35	77526.0
36	77526.0
37	77526.0
38	86939.0
39	96352.0
40	96352.0
41	96352.0
42	108243.0
43	120134.0
44	120134.0
45	120134.0
46	133013.5
47	145893.0
48	145893.0
49	145893.0
50	147213.5
51	148534.0
52	148534.0
53	148534.0
54	136507.0
55	124480.0
56	124480.0
57	124480.0
58	113999.5
59	103519.0
60	103519.0
61	103519.0
62	90258.5
63	76998.0
64	76998.0
65	76998.0
66	62832.5
67	48667.0
68	48667.0
69	48667.0
70	36619.5
71	24572.0
72	24572.0
73	24572.0
74	18507.0
75	12442.0
76	12442.0
77	12442.0
78	10121.5
79	7801.0
80	7801.0
81	7801.0
82	5312.5
83	2824.0
84	2824.0
85	2824.0
86	2222.0
87	1620.0
88	1620.0
89	1620.0
90	1119.5
91	619.0
92	619.0
93	619.0
94	437.0
95	255.0
96	255.0
97	255.0
98	398.5
99	542.0
100	542.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038099258405984264
2	0.004650613702138925
3	0.0016098278199711661
4	0.0033090905188296197
5	0.00930122740427785
6	0.013325796954205766
7	0.023700242905131057
8	0.03953021646818086
9	0.04945748802466972
10	0.05884815030783486
11	0.05670171321453996
12	0.06591350573993053
13	0.0653768964666068
14	0.06940146601653471
15	0.06251498034221362
16	0.06877542186432371
17	0.06528746158771952
18	0.07762947487416512
19	0.07458868899199736
20	0.07932873757302358
21	0.07512529826532109
22	0.07879212829969985
23	0.08263782809185319
24	0.07852382366303799
25	0.07843438878415071
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1118132.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.341324214107246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.22829827402626	43.84598941714879
2	12.58209174415375	13.926192370097096
3	3.610466618470692	5.9942401109099395
4	1.504520366100868	3.3304859746846183
5	0.8260011289606314	2.2855998139514453
6	0.4865332800075569	1.6155237593910736
7	0.31792091306326775	1.2315915026995512
8	0.2245389972915399	0.9941028358257326
9	0.16148351716969941	0.8043040511030419
>10	0.873663169660937	9.034436155658419
>50	0.0916892316907095	3.5898622115361998
>100	0.08354592978061764	9.2776084674738
>500	0.007949028974571774	3.0941818165195594
>1k	0.0012978006489096775	0.975881513000656
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2525	0.2258230691903997	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1861	0.16643830960924114	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1221	0.10919998712137745	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1120	0.10016706435376145	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.943487888728702E-5
2	0.0	0.0	0.0	0.0	8.943487888728702E-5
3	0.0	0.0	0.0	0.0	8.943487888728702E-5
4	0.0	0.0	0.0	0.0	8.943487888728702E-5
5	0.0	0.0	0.0	0.0	8.943487888728702E-5
6	0.0	0.0	0.0	0.0	8.943487888728702E-5
7	0.0	0.0	0.0	0.0	8.943487888728702E-5
8	0.0	0.0	0.0	8.943487888728702E-5	8.943487888728702E-5
9	0.0	0.0	0.0	8.943487888728702E-5	8.943487888728702E-5
10	0.0	0.0	0.0	8.943487888728702E-5	8.943487888728702E-5
11	0.0	0.0	0.0	8.943487888728702E-5	8.943487888728702E-5
12	0.0	0.0	0.0	8.943487888728702E-5	3.5773951554914806E-4
13	0.0	0.0	0.0	8.943487888728702E-5	4.4717439443643506E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGCG	25	0.0060355547	18.99754	4
GGACCGA	55	1.1272139E-5	15.546223	6
GCTCGTA	70	4.4280023E-7	14.929977	9
ATCCCGA	40	0.005276143	14.250705	16
TCCAACG	125	7.2759576E-12	13.679452	18
GGCGAGG	260	0.0	13.153318	19
AAGCTCG	80	1.9958552E-6	13.06373	7
CGACCCG	95	7.383278E-8	12.999479	5
TCGCGTA	90	5.394231E-7	12.66786	9
CCATTAA	90	5.39716E-7	12.667293	1
ACAATAC	90	5.411839E-7	12.664459	3
CGGTCGG	100	1.439239E-7	12.351163	10
TGATACT	85	3.9462266E-6	12.292525	4
GCGTTAT	95	1.0382737E-6	12.000593	1
CGCATCG	160	3.6379788E-12	11.874524	13
CCAACGA	80	2.8658462E-5	11.874524	19
TTACCCT	80	2.868472E-5	11.873462	4
ATAAGGG	65	8.0320647E-4	11.690269	3
AACAATA	90	7.46968E-6	11.609088	2
GTATCAA	1755	0.0	11.530485	1
>>END_MODULE