FastQCFastQC Report
Thu 2 Feb 2017
SRR4063956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118132
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20850.021.4171371
GTCCTAC7950.021.3056641
TCCTACA8600.021.2291052
GTATTAC1351.1277734E-1021.1826881
TATAGGA1351.1277734E-1021.181742
GTCCTAA3950.021.1620621
GATATAC3750.020.5309141
GATCGGT654.9357675E-420.30536
CGGATCG550.00448298220.00032626
GTCTACG550.004486464419.9976431
TATAAGG1452.9649527E-1019.720932
CCTACAG9300.019.6320933
TAGGACG7450.019.4876354
GTATTAG2600.019.4592461
ATCGATT808.986678E-519.25031329
TGTAGGA8600.018.9271532
GTAGGAC8050.018.581663
TCTAGAT2850.018.5233022
TAACGGC1203.156456E-718.33609236
TGTACTA600.00741680618.3311715