##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063956_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1118132
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.311722587315273	32.0	32.0	32.0	32.0	32.0
2	31.355949029273823	32.0	32.0	32.0	32.0	32.0
3	31.447246836688333	32.0	32.0	32.0	32.0	32.0
4	31.53314277741805	32.0	32.0	32.0	32.0	32.0
5	31.46746716845596	32.0	32.0	32.0	32.0	32.0
6	35.05527880429144	36.0	36.0	36.0	36.0	36.0
7	35.070744777897424	36.0	36.0	36.0	36.0	36.0
8	35.021247938526045	36.0	36.0	36.0	36.0	36.0
9	35.14772853294602	36.0	36.0	36.0	36.0	36.0
10	34.98554285182787	36.0	36.0	36.0	36.0	36.0
11	35.14394186017393	36.0	36.0	36.0	36.0	36.0
12	35.04178308106735	36.0	36.0	36.0	36.0	36.0
13	35.09209377783661	36.0	36.0	36.0	36.0	36.0
14	35.03544304250303	36.0	36.0	36.0	36.0	36.0
15	34.996743676059715	36.0	36.0	36.0	36.0	36.0
16	35.013143349801275	36.0	36.0	36.0	36.0	36.0
17	34.96817280965038	36.0	36.0	36.0	36.0	36.0
18	34.97691238601524	36.0	36.0	36.0	36.0	36.0
19	34.97194964458579	36.0	36.0	36.0	36.0	36.0
20	34.965950352910035	36.0	36.0	36.0	36.0	36.0
21	34.956013243516864	36.0	36.0	36.0	36.0	36.0
22	34.93856449864595	36.0	36.0	36.0	36.0	36.0
23	34.89466270529776	36.0	36.0	36.0	32.0	36.0
24	34.864122482855336	36.0	36.0	36.0	32.0	36.0
25	34.832325700364535	36.0	36.0	36.0	32.0	36.0
26	34.78357921962702	36.0	36.0	36.0	32.0	36.0
27	34.76288488300129	36.0	36.0	36.0	32.0	36.0
28	34.744689356891676	36.0	36.0	36.0	32.0	36.0
29	34.716097026111406	36.0	36.0	36.0	32.0	36.0
30	34.691320881613265	36.0	36.0	36.0	32.0	36.0
31	34.677907438477746	36.0	36.0	36.0	32.0	36.0
32	34.648884925930034	36.0	36.0	36.0	32.0	36.0
33	34.62957056948554	36.0	36.0	36.0	32.0	36.0
34	34.61375848289826	36.0	36.0	36.0	32.0	36.0
35	34.57510025649923	36.0	36.0	36.0	32.0	36.0
36	34.54262913502163	36.0	36.0	36.0	32.0	36.0
37	34.52507127959847	36.0	36.0	36.0	32.0	36.0
38	34.49199647268838	36.0	36.0	36.0	32.0	36.0
39	34.47562810115443	36.0	36.0	36.0	32.0	36.0
40	34.45313612346306	36.0	36.0	36.0	32.0	36.0
41	34.43968869507357	36.0	36.0	36.0	32.0	36.0
42	34.38594101590868	36.0	36.0	36.0	32.0	36.0
43	34.36809786322188	36.0	36.0	36.0	32.0	36.0
44	34.301950932448044	36.0	36.0	36.0	32.0	36.0
45	34.27564545152093	36.0	36.0	36.0	32.0	36.0
46	34.232486862016295	36.0	36.0	36.0	32.0	36.0
47	34.21747074585112	36.0	36.0	36.0	32.0	36.0
48	34.16252821670429	36.0	36.0	36.0	32.0	36.0
49	34.13459502098142	36.0	36.0	36.0	32.0	36.0
50	33.52705494521219	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	14.0
21	30.0
22	97.0
23	277.0
24	844.0
25	1890.0
26	3993.0
27	7273.0
28	12708.0
29	19657.0
30	29415.0
31	41746.0
32	62086.0
33	100384.0
34	206070.0
35	631644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1103291843912	18.440193339033804	12.165976766401082	26.28350071017391
2	15.494723775163688	20.733117556203265	37.96959027089003	25.802568397743013
3	18.238202858162417	24.672958714139938	28.92675366303233	28.16208476466532
4	12.164127312338794	16.4863361392036	36.340610947544654	35.008925600912946
5	13.965703512644303	37.31527225765831	33.621701194492246	15.097323035205147
6	33.73093134883122	36.194059551161715	16.97064118605065	13.104367913956413
7	29.71082994747485	30.67735354801897	21.00818240438732	18.60363410011886
8	27.501046388082983	34.00153112512655	19.34995152629564	19.147470960494825
9	27.40734447107645	14.154294708975781	18.691106500196756	39.747254319751015
10	15.851311395357573	27.086489667496032	31.762544695480454	25.299654241665937
11	36.92497844619419	21.056547885222855	23.100313737555137	18.918159931027823
12	24.989983293564624	23.720812927275134	29.21613906050449	22.073064718655758
13	29.680054519697208	19.885558719574146	25.325096947755533	25.109289812973113
14	23.28946238713278	20.274870140091295	25.46184336677017	30.973824106005754
15	24.700110904407556	28.243327847738982	22.325772753291357	24.730788494562105
16	24.999776411602987	26.018802889835314	24.714119875577527	24.267300822984172
17	23.45286603012882	26.327392472445116	25.73801662057789	24.481724876848173
18	24.171382882818428	25.333772162042273	27.07793740967045	23.416907545468852
19	25.613344399409016	24.798771522503603	25.59116454944497	23.996719528642416
20	25.69408856465445	24.838188353496626	25.294221287320422	24.173501794528498
21	26.559118243642075	24.369752408481286	24.623031985490083	24.44809736238655
22	25.707519326877325	24.94929042367091	25.2251075901593	24.118082659292465
23	24.193603202512783	24.81258753517058	25.917833653214945	25.075975609101693
24	24.908798374424375	25.070229052692582	25.76183206379955	24.259140509083494
25	24.639814686456102	25.054981419455242	25.81734273614732	24.48786115794134
26	23.884926189443366	25.9063650145564	26.34730538922156	23.861403406778674
27	24.70330224666542	25.34530887065686	25.419813122229012	24.531575760448707
28	23.98484909465738	25.41345246559966	25.90974747000452	24.691950969738436
29	24.300824075084385	25.04243798866293	25.72913741322348	24.927600523029206
30	24.08795356366661	25.33516979849565	26.055237056050945	24.521639581786797
31	24.92784344240668	25.008877023044484	25.21960010911807	24.84367942543077
32	24.482870880496847	25.51999112777436	25.069582576093648	24.92755541563515
33	24.037324632719244	25.268003599020467	25.768503562350524	24.926168205909764
34	24.759302569973304	25.338496831692908	25.946400382790525	23.955800215543263
35	25.081790683821993	25.351445043278854	25.796218220585317	23.77054605231384
36	24.038452938261713	25.60503093801485	25.56961187504584	24.7869042486776
37	24.84822412386772	25.550847154448274	25.12449735979737	24.476431361886636
38	24.396269212055298	25.59281891296259	25.508296602650333	24.50261527233178
39	25.24383339638791	24.945375058470823	25.063255816449466	24.7475357286918
40	25.709709068696064	25.165928956201554	25.45123464022417	23.673127334878217
41	24.427258343939247	25.407325190258	26.130793042044136	24.034623423758617
42	25.333150882916915	26.031113162187687	25.28601785867605	23.34971809621934
43	24.435703566171014	25.29419971773999	25.655878896502877	24.614217819586127
44	24.05857684454545	25.756604160014884	25.321499193736635	24.863319801703035
45	24.252741876773623	25.854357444319533	25.35280170929622	24.540098969610625
46	24.21803800496728	25.274725274725274	25.29118151320479	25.216055207102656
47	24.961363303008298	25.137196049039982	25.521595409388002	24.379845238563718
48	25.107369068764502	25.837085213745386	24.591138943892616	24.464406773597496
49	24.623320964434274	25.77882476677515	24.965858082310866	24.63199618647971
50	24.480061961481567	26.324686033803353	24.624501616114276	24.570750388600803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	16.5
2	14.0
3	42.5
4	71.0
5	101.0
6	131.0
7	125.0
8	119.0
9	185.5
10	252.0
11	376.0
12	500.0
13	976.0
14	1452.0
15	1975.5
16	2499.0
17	2789.0
18	3079.0
19	3183.5
20	3288.0
21	3633.0
22	3978.0
23	4578.0
24	5178.0
25	6234.5
26	7291.0
27	9737.5
28	12184.0
29	14579.0
30	16974.0
31	19272.0
32	21570.0
33	25848.5
34	30127.0
35	35244.0
36	40361.0
37	45129.0
38	49897.0
39	51485.5
40	53074.0
41	56067.5
42	59061.0
43	59538.5
44	60016.0
45	65475.0
46	70934.0
47	75487.5
48	80041.0
49	82395.0
50	84749.0
51	82034.0
52	79319.0
53	78246.5
54	77174.0
55	77348.5
56	77523.0
57	75678.0
58	73833.0
59	66604.0
60	59375.0
61	51832.0
62	44289.0
63	38201.5
64	32114.0
65	26901.5
66	21689.0
67	18513.5
68	15338.0
69	13646.0
70	11954.0
71	9219.0
72	6484.0
73	5497.5
74	4511.0
75	3562.5
76	2614.0
77	2262.5
78	1911.0
79	1589.5
80	1268.0
81	1015.0
82	762.0
83	664.5
84	567.0
85	408.0
86	249.0
87	180.5
88	112.0
89	93.0
90	74.0
91	56.5
92	39.0
93	32.5
94	26.0
95	20.5
96	15.0
97	15.5
98	16.0
99	16.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008585748373179554
2	2.6830463666186106E-4
3	8.943487888728702E-5
4	0.0
5	0.0
6	8.943487888728702E-5
7	8.943487888728702E-5
8	0.0
9	0.0010732185466474442
10	8.943487888728701E-4
11	0.0
12	0.0
13	3.5773951554914806E-4
14	3.5773951554914806E-4
15	0.004650613702138925
16	5.366092733237221E-4
17	0.0
18	3.5773951554914806E-4
19	0.0
20	9.83783667760157E-4
21	0.0
22	0.0
23	8.943487888728701E-4
24	4.4717439443643506E-4
25	0.0024147417299567493
26	0.00599213688544823
27	0.006707615916546525
28	0.004203439307702489
29	0.00268304636661861
30	0.0037562649132660544
31	0.0068864856743211
32	0.0032196556399423323
33	0.004471743944364351
34	0.0024147417299567493
35	0.0030407858821677584
36	0.00804913909985583
37	0.0032196556399423323
38	0.007512529826532109
39	0.004740048581026212
40	0.0029513510032804714
41	0.004024569549927915
42	0.0017886975777457403
43	0.0012520883044220181
44	9.83783667760157E-4
45	9.83783667760157E-4
46	0.001341523183309305
47	0.0021464370932948885
48	0.0023253068510694624
49	4.4717439443643506E-4
50	0.0016098278199711661
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1118132.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.33922643266424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10651059767038	47.5345837108578
2	12.304698288204804	14.603025558188017
3	3.5044374142035837	6.238518157215805
4	1.3825366961727823	3.2815463226265695
5	0.7416910997548755	2.2005688055723156
6	0.433788856342233	1.5444417102274934
7	0.2822437931262717	1.1723689844673713
8	0.1959673492476005	0.9302840728329889
9	0.14243063884617654	0.7606551536498063
>10	0.7591481586434594	8.280094165656438
>50	0.0738991830700854	3.0594047390253247
>100	0.06642962842922379	7.834535688420444
>500	0.005763298998970474	2.2772014441786887
>1k	4.549972893924059E-4	0.2827714870809564
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.943487888728702E-5
2	0.0	0.0	0.0	0.0	8.943487888728702E-5
3	0.0	0.0	0.0	0.0	8.943487888728702E-5
4	0.0	0.0	0.0	0.0	8.943487888728702E-5
5	0.0	0.0	0.0	0.0	8.943487888728702E-5
6	0.0	0.0	0.0	0.0	1.7886975777457403E-4
7	0.0	0.0	0.0	0.0	2.68304636661861E-4
8	0.0	0.0	0.0	0.0	2.68304636661861E-4
9	0.0	0.0	0.0	0.0	2.68304636661861E-4
10	0.0	0.0	0.0	0.0	2.68304636661861E-4
11	0.0	0.0	0.0	0.0	2.68304636661861E-4
12	0.0	0.0	0.0	0.0	7.154790310982961E-4
13	0.0	0.0	0.0	0.0	9.83783667760157E-4
14	0.0	0.0	0.0	0.0	9.83783667760157E-4
15	0.0	0.0	0.0	0.0	0.0011626534255347312
16	0.0	0.0	0.0	0.0	0.0011626534255347312
17	0.0	0.0	0.0	0.0	0.0011626534255347312
18	0.0	0.0	0.0	0.0	0.0011626534255347312
19	0.0	0.0	0.0	0.0	0.0011626534255347312
20	0.0	0.0	0.0	1.7886975777457403E-4	0.0011626534255347312
21	0.0	0.0	0.0	1.7886975777457403E-4	0.0011626534255347312
22	0.0	0.0	0.0	1.7886975777457403E-4	0.0014309580621965922
23	0.0	0.0	0.0	2.68304636661861E-4	0.0014309580621965922
24	0.0	0.0	0.0	3.5773951554914806E-4	0.0014309580621965922
25	0.0	0.0	0.0	5.36609273323722E-4	0.0014309580621965922
26	0.0	0.0	0.0	5.36609273323722E-4	0.0015203929410838792
27	0.0	0.0	0.0	7.154790310982961E-4	0.0015203929410838792
28	0.0	0.0	0.0	0.0011626534255347312	0.0015203929410838792
29	0.0	0.0	0.0	0.0025936114877313232	0.0015203929410838792
30	0.0	0.0	0.0	0.00599213688544823	0.0015203929410838792
31	0.0	0.0	0.0	0.012610317923107468	0.0016098278199711661
32	0.0	0.0	0.0	0.02092776165962516	0.0016992626988584533
33	0.0	0.0	0.0	0.030139554185015724	0.0016992626988584533
34	0.0	0.0	0.0	0.04069286989371559	0.0017886975777457403
35	0.0	0.0	0.0	0.053213752937935774	0.0017886975777457403
36	0.0	0.0	0.0	0.068238812591	0.0017886975777457403
37	0.0	0.0	0.0	0.09480097162052423	0.0017886975777457403
38	0.0	0.0	0.0	0.13263192538984664	0.0017886975777457403
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2085	0.0	21.417137	1
GTCCTAC	795	0.0	21.305664	1
TCCTACA	860	0.0	21.229105	2
GTATTAC	135	1.1277734E-10	21.182688	1
TATAGGA	135	1.1277734E-10	21.18174	2
GTCCTAA	395	0.0	21.162062	1
GATATAC	375	0.0	20.530914	1
GATCGGT	65	4.9357675E-4	20.3053	6
CGGATCG	55	0.004482982	20.000326	26
GTCTACG	55	0.0044864644	19.997643	1
TATAAGG	145	2.9649527E-10	19.72093	2
CCTACAG	930	0.0	19.632093	3
TAGGACG	745	0.0	19.487635	4
GTATTAG	260	0.0	19.459246	1
ATCGATT	80	8.986678E-5	19.250313	29
TGTAGGA	860	0.0	18.927153	2
GTAGGAC	805	0.0	18.58166	3
TCTAGAT	285	0.0	18.523302	2
TAACGGC	120	3.156456E-7	18.336092	36
TGTACTA	60	0.007416806	18.331171	5
>>END_MODULE