FastQCFastQC Report
Thu 2 Feb 2017
SRR4063955_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063955_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235614
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3930.16679823779571673No Hit
TATCAACGCAGAGTACTTTTTTTTT2930.12435593810215012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA402.748894E-416.6248134
TCTAAGG350.00216301916.2855323
GTAAATG350.00216301916.2855323
ATTATAC456.732819E-414.7776123
CTGGACA456.732819E-414.7776124
AACAAGG400.005261918514.2498417
TCAGAAG500.001493096213.2998523
GTATCAA2950.012.88668251
GGTATCA1155.1932147E-912.3964291
GTTACCC550.003055433512.0907753
CTACACT657.979921E-411.6921774
CATAGGA600.005860360311.0808572
CTACAGT909.436853E-510.5554374
TACAGTG1105.974769E-610.3635225
TCCTACA750.002638227310.131072
GCTCAGA900.00110196799.5039281
TATCAAC4450.08.5374172
ATCAACG4800.07.91657833
TCAACGC4850.07.83496434
AGTACTT1855.2869946E-67.702616712
Produced by FastQC (version 0.11.5)