FastQCFastQC Report
Thu 2 Feb 2017
SRR4063955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235614
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAAG250.002348962535.22
GTATCAA3100.034.0645181
GGTATCA900.031.7777791
ACACGCT300.0057357529.3333369
ACCTATG407.015225E-427.5000027
TATACTC407.015225E-427.5000025
ATGAATT508.313479E-526.422
CCATAAT450.001394879124.4444451
TGCAATC551.5883149E-424.030
CTAGAAC500.002574333522.03
GTAGGAC500.002574333522.03
TTATACT500.002574333522.04
TATCAAC4600.021.9999982
ATCAACG4600.021.9999983
TCAACGC4800.021.0833364
CAACGCA4850.020.8659785
AACGCAG4950.020.4444456
TATTATG550.004473090620.02
TAGGACA550.004473090620.04
TAACTCA550.004473090620.043
Produced by FastQC (version 0.11.5)