##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063955_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235614
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.371510181907695	32.0	32.0	32.0	32.0	32.0
2	31.495403498943187	32.0	32.0	32.0	32.0	32.0
3	31.536839916134017	32.0	32.0	32.0	32.0	32.0
4	31.599654519680495	32.0	32.0	32.0	32.0	32.0
5	31.590873207873894	32.0	32.0	32.0	32.0	32.0
6	35.16716324157308	36.0	36.0	36.0	36.0	36.0
7	35.18575721306883	36.0	36.0	36.0	36.0	36.0
8	35.14479614963457	36.0	36.0	36.0	36.0	36.0
9	35.22015669697047	36.0	36.0	36.0	36.0	36.0
10	35.12003531199335	36.0	36.0	36.0	36.0	36.0
11	35.220182162350284	36.0	36.0	36.0	36.0	36.0
12	35.161153411936475	36.0	36.0	36.0	36.0	36.0
13	35.16978617569414	36.0	36.0	36.0	36.0	36.0
14	35.1302639062195	36.0	36.0	36.0	36.0	36.0
15	35.122938365292384	36.0	36.0	36.0	36.0	36.0
16	35.11849890074444	36.0	36.0	36.0	36.0	36.0
17	35.096484079893386	36.0	36.0	36.0	36.0	36.0
18	35.10425526496728	36.0	36.0	36.0	36.0	36.0
19	35.098873581366135	36.0	36.0	36.0	36.0	36.0
20	35.07823389102515	36.0	36.0	36.0	36.0	36.0
21	35.07888750244043	36.0	36.0	36.0	36.0	36.0
22	35.06072644240155	36.0	36.0	36.0	36.0	36.0
23	35.02368280322901	36.0	36.0	36.0	36.0	36.0
24	35.01423090308725	36.0	36.0	36.0	36.0	36.0
25	34.98183045150118	36.0	36.0	36.0	36.0	36.0
26	34.936298352389926	36.0	36.0	36.0	36.0	36.0
27	34.91678338299082	36.0	36.0	36.0	36.0	36.0
28	34.89545612739481	36.0	36.0	36.0	36.0	36.0
29	34.878916363204226	36.0	36.0	36.0	36.0	36.0
30	34.87695128472841	36.0	36.0	36.0	32.0	36.0
31	34.847063417284204	36.0	36.0	36.0	32.0	36.0
32	34.83116028758902	36.0	36.0	36.0	32.0	36.0
33	34.80392930810563	36.0	36.0	36.0	32.0	36.0
34	34.81325812557828	36.0	36.0	36.0	32.0	36.0
35	34.76540443267378	36.0	36.0	36.0	32.0	36.0
36	34.744365784715676	36.0	36.0	36.0	32.0	36.0
37	34.746509120850206	36.0	36.0	36.0	32.0	36.0
38	34.70704627059513	36.0	36.0	36.0	32.0	36.0
39	34.71924843175702	36.0	36.0	36.0	32.0	36.0
40	34.69023063145654	36.0	36.0	36.0	32.0	36.0
41	34.686423557174024	36.0	36.0	36.0	32.0	36.0
42	34.655050209240535	36.0	36.0	36.0	32.0	36.0
43	34.626728462655024	36.0	36.0	36.0	32.0	36.0
44	34.6071328528865	36.0	36.0	36.0	32.0	36.0
45	34.55380410332153	36.0	36.0	36.0	32.0	36.0
46	34.55081616542311	36.0	36.0	36.0	32.0	36.0
47	34.51585219893555	36.0	36.0	36.0	32.0	36.0
48	34.46471347203477	36.0	36.0	36.0	32.0	36.0
49	34.45060565161663	36.0	36.0	36.0	32.0	36.0
50	33.89804086344615	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	8.0
22	18.0
23	66.0
24	177.0
25	347.0
26	813.0
27	1420.0
28	2492.0
29	3712.0
30	5401.0
31	7767.0
32	10796.0
33	16718.0
34	34811.0
35	151068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87953648287279	18.73254382613863	12.668619211341738	26.719300479646847
2	15.389218761273785	21.16309371723971	37.724573771396315	25.723113750090192
3	17.586890366830353	24.465967497548945	29.83748774473395	28.109654390886753
4	12.710619912229323	17.167061380053816	36.43331890295144	33.68899980476542
5	13.896033342670638	36.95153938220989	34.31544814824247	14.836979126877011
6	32.685663840009504	36.533907153225194	17.212050217728997	13.568378789036306
7	29.43683073514619	31.508023750811713	20.778564849987056	18.27658066405504
8	26.785759759606815	34.10535876475931	19.954247200930336	19.15463427470354
9	27.523640561601276	14.848140162640272	18.981630816766547	38.64658845899191
10	15.53789933407184	28.18883668419556	32.90381179146984	23.369452190262763
11	35.50213484767459	22.226607926523894	23.10601237617459	19.165244849626934
12	24.31444784455866	24.88402592386668	29.106628242074926	21.694897989499733
13	29.22304466665535	20.877544437464984	24.89771318948101	25.001697706398655
14	23.172222363696555	20.527218246793485	26.177986027994944	30.12257336151502
15	24.344995310026356	27.99765713533863	22.84844806437785	24.808899490257165
16	24.412386360742573	27.219944485472002	24.21969832013378	24.147970833651648
17	23.272810613970307	27.2615379391717	25.84269186041577	23.62295958644223
18	23.72407295013433	26.584696090623183	26.571963346674423	23.119267612568066
19	24.7756924461195	25.80194725270994	26.565908647194142	22.85645165397642
20	25.09273642483044	25.829959170507692	25.61732324904293	23.459981155618937
21	25.96577452952711	25.14748699143514	25.24892408770277	23.63781439133498
22	25.17762102421758	25.746347840111365	25.76884225894896	23.307188876722094
23	24.50893842418892	25.83060285554216	25.88662716669779	23.773831553571124
24	24.26648784235165	26.093212174200914	25.69085746541999	23.949442518027446
25	24.632440324606975	25.74615462972395	25.401514379817325	24.219890665851754
26	23.99904924002869	26.053369892317946	25.943862717051285	24.003718150602076
27	24.422977343531148	25.775234926105444	25.501048360398293	24.30073936996511
28	23.791414480106617	26.204341145811227	26.07870767297947	23.925536701102686
29	24.059674886464922	26.1487203429396	25.72726115190357	24.064343618691904
30	24.116006740037434	26.434274024116434	25.402046628495757	24.047672607350375
31	24.370636049152147	25.42498779685477	25.995033850463717	24.209342303529365
32	24.484105088918128	25.951784729001314	25.588896905903823	23.97521327617673
33	24.19790919470465	25.96953349490456	25.52556631282284	24.306990997567944
34	24.356776028182164	25.99040787742456	25.72471457068885	23.928101523704427
35	24.11261125518342	25.90457838689003	26.00644293251049	23.976367425416054
36	24.525787241989637	25.973573966154646	25.52026519412057	23.980373597735156
37	24.538007724629686	25.360977887186454	26.052374686982727	24.04863970120114
38	24.390368134876674	25.836930638855993	25.875556574261545	23.89714465200579
39	24.64994673333135	25.881234428518678	25.366394037528593	24.102424800621375
40	24.58288590746455	25.801864970056066	25.69448276154783	23.92076636093155
41	24.088945103265623	25.383903635730835	26.013344312114295	24.513806948889247
42	24.138296924141265	25.55123106502723	26.280405247677297	24.03006676315421
43	23.93988396127515	25.24075701049611	26.448680239886933	24.370678788341802
44	23.99346377488222	25.50952845804507	25.693731165909767	24.80327660116294
45	23.79737702134884	25.374559653664953	26.052799117185177	24.775264207801026
46	23.822216185932803	25.41296708147293	26.14764952549106	24.617167207103204
47	23.58293967599136	25.423261222947996	26.432127532245946	24.5616715688147
48	23.624734199457574	25.663281114049123	26.384391088625648	24.327593597867654
49	24.303412799803066	25.077139207089594	26.188707753816644	24.430740239290703
50	23.55737398774256	25.409571668675618	26.16165560327997	24.871398740301853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.5
2	4.0
3	13.5
4	23.0
5	25.5
6	28.0
7	31.0
8	34.0
9	45.5
10	57.0
11	85.5
12	114.0
13	191.5
14	269.0
15	368.0
16	467.0
17	479.5
18	492.0
19	463.5
20	435.0
21	517.5
22	600.0
23	758.5
24	917.0
25	1174.0
26	1431.0
27	1766.5
28	2102.0
29	2573.5
30	3045.0
31	3750.5
32	4456.0
33	5228.5
34	6001.0
35	7020.0
36	8039.0
37	8983.0
38	9927.0
39	11034.0
40	12141.0
41	13111.0
42	14081.0
43	15326.5
44	16572.0
45	17525.0
46	18478.0
47	18919.0
48	19360.0
49	19394.5
50	19429.0
51	19135.5
52	18842.0
53	18123.5
54	17405.0
55	16514.5
56	15624.0
57	14396.0
58	13168.0
59	11770.0
60	10372.0
61	8969.5
62	7567.0
63	6435.0
64	5303.0
65	4293.0
66	3283.0
67	2766.0
68	2249.0
69	1836.5
70	1424.0
71	1042.5
72	661.0
73	543.5
74	426.0
75	381.0
76	336.0
77	270.0
78	204.0
79	157.0
80	110.0
81	86.0
82	62.0
83	51.5
84	41.0
85	33.0
86	25.0
87	13.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010186151926455984
2	4.24422996935666E-4
3	4.24422996935666E-4
4	0.0
5	0.0
6	0.0
7	4.24422996935666E-4
8	0.0
9	8.48845993871332E-4
10	0.001273268990806998
11	0.0
12	4.24422996935666E-4
13	8.48845993871332E-4
14	0.0
15	0.00212211498467833
16	0.0
17	0.0
18	4.24422996935666E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	8.48845993871332E-4
24	4.24422996935666E-4
25	0.002546537981613996
26	0.005517498960163658
27	0.003395383975485328
28	0.003395383975485328
29	0.001697691987742664
30	0.0029709609785496614
31	0.008064036941777654
32	0.001697691987742664
33	0.004668652966292325
34	0.001697691987742664
35	0.0029709609785496614
36	0.005517498960163658
37	0.001697691987742664
38	0.008912882935648984
39	0.0029709609785496614
40	0.0029709609785496614
41	0.003395383975485328
42	0.00212211498467833
43	0.001273268990806998
44	0.001697691987742664
45	0.001697691987742664
46	8.48845993871332E-4
47	0.001273268990806998
48	0.00212211498467833
49	4.24422996935666E-4
50	8.48845993871332E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	235614.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.967906046810526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.36404202176515	25.661447161250717
2	17.35586210022134	15.26201828367863
3	8.466331639299192	11.167406222335385
4	4.9960884831118335	8.786701961080528
5	3.0538897314988103	6.713656839592956
6	2.064160482660262	5.445408850028729
7	1.466558892514253	4.513706651872681
8	1.0365676015040972	3.6460565531279903
9	0.7133260998855955	2.8227109446456873
>10	2.475191676515471	15.735242690225038
>50	0.006983612145986498	0.17940773472165505
>100	9.97658877998071E-4	0.06623610744002424
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	4.2442299693566595E-4	0.0
27	0.0	0.0	0.0	0.0025465379816139956	0.0
28	0.0	0.0	0.0	0.007639613944841988	0.0
29	0.0	0.0	0.0	0.0186746118651693	0.0
30	0.0	0.0	0.0	0.04074460770582393	0.0
31	0.0	0.0	0.0	0.08912882935648986	0.0
32	0.0	0.0	0.0	0.14557708794893343	0.0
33	0.0	0.0	0.0	0.20160092354444134	0.0
34	0.0	0.0	0.0	0.25847360513382056	0.0
35	0.0	0.0	0.0	0.31874167069868514	0.0
36	0.0	0.0	0.0	0.40956819204291767	0.0
37	0.0	0.0	0.0	0.5619360479428217	0.0
38	0.0	0.0	0.0	0.7885779283064673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAAAG	25	0.0023489625	35.2	2
GTATCAA	310	0.0	34.064518	1
GGTATCA	90	0.0	31.777779	1
ACACGCT	30	0.00573575	29.333336	9
ACCTATG	40	7.015225E-4	27.500002	7
TATACTC	40	7.015225E-4	27.500002	5
ATGAATT	50	8.313479E-5	26.4	22
CCATAAT	45	0.0013948791	24.444445	1
TGCAATC	55	1.5883149E-4	24.0	30
CTAGAAC	50	0.0025743335	22.0	3
GTAGGAC	50	0.0025743335	22.0	3
TTATACT	50	0.0025743335	22.0	4
TATCAAC	460	0.0	21.999998	2
ATCAACG	460	0.0	21.999998	3
TCAACGC	480	0.0	21.083336	4
CAACGCA	485	0.0	20.865978	5
AACGCAG	495	0.0	20.444445	6
TATTATG	55	0.0044730906	20.0	2
TAGGACA	55	0.0044730906	20.0	4
TAACTCA	55	0.0044730906	20.0	43
>>END_MODULE