FastQCFastQC Report
Thu 2 Feb 2017
SRR4063954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666879
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45680.27404508665596006No Hit
TATCAACGCAGAGTACTTTTTTTTT32160.19293542002748848No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21450.12868360570863271No Hit
GCGCAAGACGGACCAGAGCGAAAGC18730.11236568461178045No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17080.1024669457111164No Hit
GTCCTAAAGTGTGTATTTCTCATTT16980.1018670221413792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAC500.001499626313.2999998
CTATACC500.00150030513.2992014
ACGATCA1457.2759576E-1212.4490239
ACGAACG1457.2759576E-1212.44902315
CGTCAAT2700.012.31592419
ATTGGAC701.0925209E-412.2124523
ACGCGCG550.003066535812.09199713
CGCCAGT2350.011.72410818
GTCCTAA4950.011.7053141
GAACCGA658.027993E-411.6916066
CGAACGA1552.5465852E-1111.6458616
CGCAAGA3350.011.6240742
CGGTCCA4100.011.58571410
AAGACGG3550.011.5063515
CCGACCA2150.011.4890629
CAAGACG3400.011.4551954
CGGTCGG2000.011.40034210
GCTATAC752.076879E-411.3982893
TAACGAA1604.5474735E-1111.28226613
TTAGGAC5500.011.2255883
Produced by FastQC (version 0.11.5)