##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063954_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1666879
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289492518653123	32.0	32.0	32.0	32.0	32.0
2	30.944902419431763	32.0	32.0	32.0	32.0	32.0
3	30.958915434173687	32.0	32.0	32.0	32.0	32.0
4	30.97721070335639	32.0	32.0	32.0	32.0	32.0
5	30.90695245425733	32.0	32.0	32.0	32.0	32.0
6	34.57464159066135	36.0	36.0	36.0	32.0	36.0
7	34.507535939921254	36.0	36.0	36.0	32.0	36.0
8	34.4801068343893	36.0	36.0	36.0	32.0	36.0
9	34.59171901499749	36.0	36.0	36.0	32.0	36.0
10	34.32494500200674	36.0	36.0	36.0	32.0	36.0
11	34.57929639763894	36.0	36.0	36.0	32.0	36.0
12	34.40111729765628	36.0	36.0	36.0	32.0	36.0
13	34.482330751062314	36.0	36.0	36.0	32.0	36.0
14	34.3780796326548	36.0	36.0	36.0	32.0	36.0
15	34.3303995071028	36.0	36.0	36.0	32.0	36.0
16	34.32325381746365	36.0	36.0	36.0	32.0	36.0
17	34.26111853349883	36.0	36.0	36.0	32.0	36.0
18	34.25466875520059	36.0	36.0	36.0	32.0	36.0
19	34.26278932064055	36.0	36.0	36.0	32.0	36.0
20	34.24222394066996	36.0	36.0	36.0	32.0	36.0
21	34.21741350151991	36.0	36.0	36.0	32.0	36.0
22	34.20307832782103	36.0	36.0	36.0	32.0	36.0
23	34.15508084270064	36.0	36.0	36.0	32.0	36.0
24	34.13712453033484	36.0	36.0	36.0	32.0	36.0
25	33.75022182173991	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	14.0
5	18.0
6	72.0
7	22.0
8	84.0
9	101.0
10	140.0
11	42.0
12	114.0
13	77.0
14	166.0
15	260.0
16	494.0
17	584.0
18	784.0
19	987.0
20	1508.0
21	2191.0
22	3469.0
23	5205.0
24	7977.0
25	11799.0
26	16866.0
27	22710.0
28	30880.0
29	41760.0
30	55275.0
31	75021.0
32	107261.0
33	153342.0
34	339005.0
35	788646.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.75178069432074	17.103127828285132	11.130569846189301	26.014521631204822
2	16.500689646069677	19.647923532379767	38.16121579292499	25.690171028625564
3	19.073293381704044	23.611376915365458	28.79840945400573	28.516920248924766
4	12.419344484767619	15.195424076274994	36.08399512602209	36.301236312935295
5	14.358551299396725	36.7405736364809	33.82319324907679	15.077681815045581
6	34.12561365024546	35.70553428221371	16.846146738458696	13.322705329082133
7	29.979616071680383	30.493084645807496	20.770064848134634	18.757234434377494
8	27.774750466899356	33.74194028489262	19.346496934547673	19.136812313660347
9	27.372241525533088	13.836262441532376	18.591099056295178	40.20039697663936
10	15.772511643513699	26.79853945108764	31.915262712123845	25.513686193274825
11	37.33013205282113	20.750300120048017	22.93529411764706	18.984273709483794
12	24.801978563560453	23.474372019173337	29.36828235350616	22.355367063760053
13	29.753451268041232	19.482278278126852	25.467857899662448	25.296412554169468
14	23.136418701427182	20.127230312438844	25.250240425309673	31.486110560824304
15	24.746566461774876	28.243793219362274	22.22843834037649	24.78120197848636
16	25.107319196194076	25.74616181171492	24.888088484684765	24.25843050740624
17	23.464750889316555	26.238650183746064	25.76046620236919	24.536132724568187
18	24.16546742289568	25.035166810455845	27.310878206663773	23.4884875599847
19	25.443375616187197	24.633199394604148	25.565967675799833	24.357457313408823
20	25.488839938353546	24.69142250936134	25.410310951971837	24.409426600313274
21	26.44868784658915	24.212206661835857	24.65706548069667	24.682040010878328
22	25.72806849018996	24.79191181767969	25.09425806296679	24.385761629163564
23	24.134069894012832	24.61696503137948	25.989640839611933	25.259324234995756
24	24.859214289659544	25.008765336336765	25.739710335652344	24.392310038351347
25	24.500124878723547	24.826730771078086	25.957483597659962	24.7156607525384
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	120.0
1	120.0
2	366.5
3	613.0
4	613.0
5	613.0
6	1831.0
7	3049.0
8	3049.0
9	3049.0
10	3208.5
11	3368.0
12	3368.0
13	3368.0
14	3816.5
15	4265.0
16	4265.0
17	4265.0
18	7330.0
19	10395.0
20	10395.0
21	10395.0
22	17614.0
23	24833.0
24	24833.0
25	24833.0
26	38666.5
27	52500.0
28	52500.0
29	52500.0
30	68169.5
31	83839.0
32	83839.0
33	83839.0
34	98519.0
35	113199.0
36	113199.0
37	113199.0
38	126327.0
39	139455.0
40	139455.0
41	139455.0
42	157424.0
43	175393.0
44	175393.0
45	175393.0
46	194331.0
47	213269.0
48	213269.0
49	213269.0
50	216518.5
51	219768.0
52	219768.0
53	219768.0
54	203355.0
55	186942.0
56	186942.0
57	186942.0
58	171887.5
59	156833.0
60	156833.0
61	156833.0
62	138084.0
63	119335.0
64	119335.0
65	119335.0
66	98062.0
67	76789.0
68	76789.0
69	76789.0
70	57967.0
71	39145.0
72	39145.0
73	39145.0
74	30126.5
75	21108.0
76	21108.0
77	21108.0
78	16992.0
79	12876.0
80	12876.0
81	12876.0
82	8847.5
83	4819.0
84	4819.0
85	4819.0
86	3677.5
87	2536.0
88	2536.0
89	2536.0
90	1826.5
91	1117.0
92	1117.0
93	1117.0
94	764.0
95	411.0
96	411.0
97	411.0
98	656.5
99	902.0
100	902.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040494840957262045
2	0.004919373271845167
3	0.0017997707092116465
4	0.004079480274213065
5	0.008638899404215902
6	0.01277837203540269
7	0.02219717208027697
8	0.034015666404100114
9	0.04715399258134514
10	0.05579289198556104
11	0.052733281779901234
12	0.061432173541090866
13	0.06251203596661785
14	0.064851737888593
15	0.05753267033779896
16	0.06323194425030251
17	0.059572410474905496
18	0.07475047678925704
19	0.07151088951267608
20	0.07511043093109937
21	0.07091096594293887
22	0.074330530290441
23	0.07799006406583801
24	0.07361062200675633
25	0.07577034685781031
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1666879.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.591867703054575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18169292065473	38.54174129636493
2	13.936935948357569	14.101912393705026
3	4.383951043426834	6.6537681361715535
4	1.8529121203740426	3.7496913943739956
5	0.9690976581419946	2.45142302560299
6	0.5857362666454095	1.7780095026603389
7	0.3685688646388465	1.3052611067491484
8	0.2744846750677986	1.110935389403679
9	0.2045315225062082	0.9312868554964571
>10	1.0535114660661051	9.55965269769448
>50	0.08716050040927334	3.08941873385603
>100	0.08463348244720202	9.070575229979502
>500	0.01202224579897007	4.040018123226793
>1k	0.004761285464938641	3.616306114715104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4568	0.27404508665596006	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3216	0.19293542002748848	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2145	0.12868360570863271	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1873	0.11236568461178045	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1708	0.1024669457111164	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1698	0.1018670221413792	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.999235697372155E-5
2	0.0	0.0	0.0	0.0	5.999235697372155E-5
3	0.0	0.0	0.0	0.0	5.999235697372155E-5
4	0.0	0.0	0.0	0.0	5.999235697372155E-5
5	0.0	0.0	0.0	0.0	5.999235697372155E-5
6	0.0	0.0	0.0	5.999235697372155E-5	1.7997707092116464E-4
7	0.0	0.0	0.0	5.999235697372155E-5	1.7997707092116464E-4
8	0.0	0.0	0.0	5.999235697372155E-5	1.7997707092116464E-4
9	0.0	0.0	0.0	5.999235697372155E-5	1.7997707092116464E-4
10	0.0	0.0	0.0	5.999235697372155E-5	1.7997707092116464E-4
11	0.0	0.0	0.0	5.999235697372155E-5	1.7997707092116464E-4
12	0.0	0.0	0.0	5.999235697372155E-5	5.399312127634939E-4
13	0.0	0.0	0.0	5.999235697372155E-5	7.799006406583801E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAAC	50	0.0014996263	13.299999	8
CTATACC	50	0.001500305	13.299201	4
ACGATCA	145	7.2759576E-12	12.449023	9
ACGAACG	145	7.2759576E-12	12.449023	15
CGTCAAT	270	0.0	12.315924	19
ATTGGAC	70	1.0925209E-4	12.212452	3
ACGCGCG	55	0.0030665358	12.091997	13
CGCCAGT	235	0.0	11.724108	18
GTCCTAA	495	0.0	11.705314	1
GAACCGA	65	8.027993E-4	11.691606	6
CGAACGA	155	2.5465852E-11	11.64586	16
CGCAAGA	335	0.0	11.624074	2
CGGTCCA	410	0.0	11.585714	10
AAGACGG	355	0.0	11.506351	5
CCGACCA	215	0.0	11.489062	9
CAAGACG	340	0.0	11.455195	4
CGGTCGG	200	0.0	11.400342	10
GCTATAC	75	2.076879E-4	11.398289	3
TAACGAA	160	4.5474735E-11	11.282266	13
TTAGGAC	550	0.0	11.225588	3
>>END_MODULE