FastQCFastQC Report
Thu 2 Feb 2017
SRR4063954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666879
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17380.10426671642032805No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16760.10054719028795732No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16680.10006725143216755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31300.021.2237531
GTAGGAC11950.020.6156333
ACCGTAT654.937076E-420.3053518
TGTAGGA13050.019.3835772
GTCCTAC12050.019.3498861
GATATAC5500.019.1972121
CTGTAGG13500.018.9009591
TAGGACG11000.018.59734
TATCGCT600.007418178518.331227
TCCTACA13350.017.7946362
AGGACGT11250.017.7935075
ATAATAC2350.017.7841173
CGATTAA1002.479986E-517.60325233
CCTACAG13000.017.5969163
CGTCTTA1408.385905E-817.2842415
TTAGGAC8200.016.8994793
AACGCAG38700.016.8817776
TACACCG1053.7794576E-516.7599745
GGACGTG11700.016.5451016
GTCCTAG3200.016.4976041
Produced by FastQC (version 0.11.5)