FastQCFastQC Report
Thu 2 Feb 2017
SRR4063953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084074
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27240.25127435949944377No Hit
TATCAACGCAGAGTACTTTTTTTTT18250.16834644129459797No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16280.15017425009731808No Hit
GAATAGGACCGCGGTTCTATTTTGT12430.11466006933106042No Hit
GCGCAAGACGGACCAGAGCGAAAGC12250.11299966607445618No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12140.11198497519542025No Hit
GTATCTGATCGTCTTCGAACCTCCG11920.10995559343734837No Hit
GAACTACGACGGTATCTGATCGTCT11900.10977110418661457No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11620.10718825467634129No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11560.10663478692413987No Hit
GTCCTATTCCATTATTCCTAGCTGC11110.10248377878262922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCT402.7586642E-416.6261717
AAGACCG508.721975E-515.1975625
TAAGACT508.72541E-515.1968624
AATCGTA400.005275269514.25100313
GATAACG1051.36788E-913.57238511
GCTATAC707.2766543E-613.5679993
TTAAGAC1051.373337E-913.5679993
GTCTTAC852.7093301E-713.406521
CCAACGA1001.0028089E-813.3027819
CCGATAA957.362905E-813.0021179
CCGTCAA1501.8189894E-1212.66931418
CGATAAC1051.988883E-812.6675610
ACGAAAT604.0878146E-412.66755912
AAGGCGT604.094189E-412.6652196
TAAGGCT905.4134216E-712.6640514
TTGCACC604.0973793E-412.6640514
CGACCAT1900.012.50088110
CGCCACT701.0886554E-412.21627417
TACTCCG550.003071099512.088975
AAAGGCG550.003071099512.088975