##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063953_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084074
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.157414530742365	32.0	32.0	32.0	32.0	32.0
2	30.79550473491662	32.0	32.0	32.0	32.0	32.0
3	30.82909100301271	32.0	32.0	32.0	32.0	32.0
4	30.816251473607892	32.0	32.0	32.0	32.0	32.0
5	30.784207535647937	32.0	32.0	32.0	32.0	32.0
6	34.23761016314384	36.0	36.0	36.0	32.0	36.0
7	34.2039215035136	36.0	36.0	36.0	32.0	36.0
8	34.16324070128054	36.0	36.0	36.0	32.0	36.0
9	34.32708468241098	36.0	36.0	36.0	32.0	36.0
10	34.00964971025963	36.0	36.0	36.0	32.0	36.0
11	34.30682684023415	36.0	36.0	36.0	32.0	36.0
12	34.103696795606204	36.0	36.0	36.0	32.0	36.0
13	34.22489793132203	36.0	36.0	36.0	32.0	36.0
14	34.09962788518127	36.0	36.0	36.0	32.0	36.0
15	34.02929781546278	36.0	36.0	36.0	32.0	36.0
16	34.05563550089754	36.0	36.0	36.0	32.0	36.0
17	33.950366856875085	36.0	36.0	36.0	32.0	36.0
18	33.963968326885436	36.0	36.0	36.0	32.0	36.0
19	33.96240570293172	36.0	36.0	36.0	32.0	36.0
20	33.90674437353908	36.0	36.0	36.0	32.0	36.0
21	33.895183354641844	36.0	36.0	36.0	32.0	36.0
22	33.86746568961159	36.0	36.0	36.0	32.0	36.0
23	33.83794648704793	36.0	36.0	36.0	32.0	36.0
24	33.79541894741503	36.0	36.0	36.0	32.0	36.0
25	33.15654835371017	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	15.0
5	12.0
6	43.0
7	11.0
8	56.0
9	77.0
10	108.0
11	37.0
12	53.0
13	59.0
14	151.0
15	376.0
16	567.0
17	818.0
18	969.0
19	1269.0
20	1541.0
21	2046.0
22	2811.0
23	3781.0
24	5265.0
25	7557.0
26	10547.0
27	14614.0
28	20646.0
29	29214.0
30	41084.0
31	60521.0
32	92537.0
33	139013.0
34	282722.0
35	365551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.277403792472455	17.500482156517695	11.387014348424294	25.835099702585556
2	16.392902740160732	19.704360789060022	38.323874748160556	25.57886172261869
3	18.876308516565626	23.859464306140296	28.675930674912276	28.588296502381805
4	12.248555862432866	15.126960494004823	36.3956798562412	36.228803787321105
5	14.197283718733336	36.79433791336019	33.98073030732983	15.02764806057665
6	34.086673653600606	35.792967798599165	16.854348259293975	13.266010288506248
7	29.9987913311688	30.426899985883487	20.991256065257044	18.58305261769067
8	27.650670672739757	33.591679523995836	19.50875159640044	19.248898206863966
9	26.962197162456864	14.209680649498912	18.74517208823277	40.082950099811455
10	15.451800056485391	26.790872994630195	32.00515752224805	25.752169426636367
11	37.17508075680664	20.855191508998615	23.03313336409783	18.936594370096905
12	24.766658790170133	23.48937411389414	29.491725986531193	22.252241109404537
13	29.388075709662708	19.496271928610064	25.706339844305255	25.409312517421974
14	23.003568534131837	20.099450043291323	25.48381975302716	31.413161669549677
15	24.554497299801834	28.201033929921298	22.392769689848713	24.851699080428155
16	25.153409909061708	25.87984551935992	24.735086378455414	24.231658193122954
17	23.34440035480926	26.230410033607193	25.95248841839433	24.472701193189213
18	24.16340645199407	24.996445919823643	27.386541759980503	23.453605868201784
19	25.32412685371944	24.829340115111766	25.731033559649035	24.11549947151976
20	25.359292492891694	24.734130940511797	25.47228684317418	24.434289723422324
21	26.230962522316936	24.33658463634702	24.74064350320976	24.691809338126284
22	25.39676824967782	24.88996096937739	25.355226669325326	24.358044111619456
23	23.77416564423408	24.607898257110175	26.19938497102569	25.418551127630053
24	24.56161891055318	25.116616985784407	25.90914168236792	24.412622421294497
25	24.408175322637224	24.938703543009066	25.95212599006702	24.70099514428669
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	88.0
2	252.5
3	417.0
4	417.0
5	417.0
6	1381.0
7	2345.0
8	2345.0
9	2345.0
10	2395.5
11	2446.0
12	2446.0
13	2446.0
14	2669.5
15	2893.0
16	2893.0
17	2893.0
18	5065.0
19	7237.0
20	7237.0
21	7237.0
22	12034.0
23	16831.0
24	16831.0
25	16831.0
26	25725.0
27	34619.0
28	34619.0
29	34619.0
30	44287.5
31	53956.0
32	53956.0
33	53956.0
34	64449.5
35	74943.0
36	74943.0
37	74943.0
38	83706.0
39	92469.0
40	92469.0
41	92469.0
42	103685.0
43	114901.0
44	114901.0
45	114901.0
46	127594.0
47	140287.0
48	140287.0
49	140287.0
50	141570.0
51	142853.0
52	142853.0
53	142853.0
54	131666.5
55	120480.0
56	120480.0
57	120480.0
58	110411.0
59	100342.0
60	100342.0
61	100342.0
62	88423.5
63	76505.0
64	76505.0
65	76505.0
66	62530.5
67	48556.0
68	48556.0
69	48556.0
70	36264.0
71	23972.0
72	23972.0
73	23972.0
74	18520.0
75	13068.0
76	13068.0
77	13068.0
78	10854.5
79	8641.0
80	8641.0
81	8641.0
82	5903.0
83	3165.0
84	3165.0
85	3165.0
86	2450.5
87	1736.0
88	1736.0
89	1736.0
90	1194.5
91	653.0
92	653.0
93	653.0
94	408.0
95	163.0
96	163.0
97	163.0
98	335.5
99	508.0
100	508.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0369900947721281
2	0.00461223126834515
3	0.002029381758071866
4	0.003320806513208508
5	0.008486505533755075
6	0.013467715303567837
7	0.02186197621195601
8	0.035606425391624556
9	0.0489818960698255
10	0.05608473222307702
11	0.05294841496060232
12	0.06291083450022784
13	0.06198838824655881
14	0.06604715176270255
15	0.058575337107983405
16	0.0647557270075659
17	0.06226512212265952
18	0.07490263579792523
19	0.07185856316081744
20	0.0769320175559971
21	0.07527161429939284
22	0.07582508205159426
23	0.08052955794530632
24	0.07628630517842878
25	0.07508712504865904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1084074.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.73452027588937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70887530322601	42.293836558736466
2	12.779139168670946	13.73361825534722
3	3.754775010980877	6.05283101876864
4	1.556262318451591	3.3450003642175847
5	0.8145543096432536	2.188484253366924
6	0.5222972488347319	1.6839235264530688
7	0.33208687455265606	1.2491170225804502
8	0.2333100727905408	1.0029443869546033
9	0.16596234661612946	0.802611637135073
>10	0.9427424954400571	9.264752401090991
>50	0.08779229598096568	3.34613374090214
>100	0.0906940325262175	9.962411384697262
>500	0.008931987445807421	3.2390573137505743
>1k	0.0025765348401367563	1.8352781359991002
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2724	0.25127435949944377	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1825	0.16834644129459797	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1628	0.15017425009731808	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1243	0.11466006933106042	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1225	0.11299966607445618	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1214	0.11198497519542025	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1192	0.10995559343734837	No Hit
GAACTACGACGGTATCTGATCGTCT	1190	0.10977110418661457	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1162	0.10718825467634129	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1156	0.10663478692413987	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1111	0.10248377878262922	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.2244625366903E-5
2	0.0	0.0	0.0	0.0	9.2244625366903E-5
3	9.2244625366903E-5	0.0	0.0	0.0	9.2244625366903E-5
4	9.2244625366903E-5	0.0	0.0	0.0	9.2244625366903E-5
5	9.2244625366903E-5	0.0	0.0	0.0	9.2244625366903E-5
6	9.2244625366903E-5	0.0	0.0	0.0	9.2244625366903E-5
7	9.2244625366903E-5	0.0	0.0	0.0	9.2244625366903E-5
8	9.2244625366903E-5	0.0	0.0	9.2244625366903E-5	9.2244625366903E-5
9	9.2244625366903E-5	0.0	0.0	1.84489250733806E-4	9.2244625366903E-5
10	9.2244625366903E-5	0.0	0.0	1.84489250733806E-4	9.2244625366903E-5
11	9.2244625366903E-5	0.0	0.0	1.84489250733806E-4	9.2244625366903E-5
12	9.2244625366903E-5	0.0	0.0	1.84489250733806E-4	2.76733876100709E-4
13	9.2244625366903E-5	0.0	0.0	1.84489250733806E-4	3.68978501467612E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCT	40	2.7586642E-4	16.626171	7
AAGACCG	50	8.721975E-5	15.197562	5
TAAGACT	50	8.72541E-5	15.196862	4
AATCGTA	40	0.0052752695	14.251003	13
GATAACG	105	1.36788E-9	13.572385	11
GCTATAC	70	7.2766543E-6	13.567999	3
TTAAGAC	105	1.373337E-9	13.567999	3
GTCTTAC	85	2.7093301E-7	13.40652	1
CCAACGA	100	1.0028089E-8	13.30278	19
CCGATAA	95	7.362905E-8	13.002117	9
CCGTCAA	150	1.8189894E-12	12.669314	18
CGATAAC	105	1.988883E-8	12.66756	10
ACGAAAT	60	4.0878146E-4	12.667559	12
AAGGCGT	60	4.094189E-4	12.665219	6
TAAGGCT	90	5.4134216E-7	12.664051	4
TTGCACC	60	4.0973793E-4	12.664051	4
CGACCAT	190	0.0	12.500881	10
CGCCACT	70	1.0886554E-4	12.216274	17
TACTCCG	55	0.0030710995	12.08897	5
AAAGGCG	55	0.0030710995	12.08897	5
>>END_MODULE