FastQCFastQC Report
Thu 2 Feb 2017
SRR4063953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084074
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12670.1168739403398661No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11840.10921763643441315No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11340.10460540516606799No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11180.10312949116019754No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG11140.10276051265872994No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11020.1016535771543271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA3850.021.71756636
ATACGAA3750.021.70794140
CGTCGTA2750.020.79738410
CTAGCGG4150.020.14576129
ACTGTTC4150.020.1411178
CCCTTAC1101.267017E-719.9974841
ACTATAC1101.2676355E-719.9965633
TCTAGCG4200.019.9059328
GATATAC3000.019.797511
TAGCGGC4250.019.6726530
CGAATGC4050.019.55580143
CAATACG4300.019.44479638
GCAATAC4350.019.22129437
GCGCAAT4400.019.00286935
ACCGTCG2900.018.9622578
AATACGA4450.018.78848639
GTCGTAG3050.018.7517411
TACGAAT4350.018.71460541
GTCCTAC7200.018.6365451
GTATACC600.00741897418.3301814
Produced by FastQC (version 0.11.5)