##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063953_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084074
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.118990954492038	32.0	32.0	32.0	32.0	32.0
2	30.967719915799105	32.0	32.0	32.0	32.0	32.0
3	31.227013100581694	32.0	32.0	32.0	32.0	32.0
4	31.38561205231377	32.0	32.0	32.0	32.0	32.0
5	31.2569077387706	32.0	32.0	32.0	32.0	32.0
6	34.83739670908074	36.0	36.0	36.0	32.0	36.0
7	34.88082178891847	36.0	36.0	36.0	32.0	36.0
8	34.77655584397375	36.0	36.0	36.0	32.0	36.0
9	34.98489863238118	36.0	36.0	36.0	36.0	36.0
10	34.73139564273288	36.0	36.0	36.0	32.0	36.0
11	34.99036043664916	36.0	36.0	36.0	36.0	36.0
12	34.85098249750478	36.0	36.0	36.0	32.0	36.0
13	34.91894741502886	36.0	36.0	36.0	32.0	36.0
14	34.850112630687576	36.0	36.0	36.0	32.0	36.0
15	34.78522591631199	36.0	36.0	36.0	32.0	36.0
16	34.81272680647262	36.0	36.0	36.0	32.0	36.0
17	34.771149386481	36.0	36.0	36.0	32.0	36.0
18	34.77757699197656	36.0	36.0	36.0	32.0	36.0
19	34.77392779459705	36.0	36.0	36.0	32.0	36.0
20	34.76719209205276	36.0	36.0	36.0	32.0	36.0
21	34.76121648522149	36.0	36.0	36.0	32.0	36.0
22	34.745571796759265	36.0	36.0	36.0	32.0	36.0
23	34.681483920839355	36.0	36.0	36.0	32.0	36.0
24	34.65662860653424	36.0	36.0	36.0	32.0	36.0
25	34.61882583661263	36.0	36.0	36.0	32.0	36.0
26	34.54490099384359	36.0	36.0	36.0	32.0	36.0
27	34.546610286751644	36.0	36.0	36.0	32.0	36.0
28	34.504462794975254	36.0	36.0	36.0	32.0	36.0
29	34.46760276512489	36.0	36.0	36.0	32.0	36.0
30	34.4462970240039	36.0	36.0	36.0	32.0	36.0
31	34.449730368960054	36.0	36.0	36.0	32.0	36.0
32	34.39734187887543	36.0	36.0	36.0	32.0	36.0
33	34.37276791067769	36.0	36.0	36.0	32.0	36.0
34	34.366860564869185	36.0	36.0	36.0	32.0	36.0
35	34.32236083514594	36.0	36.0	36.0	32.0	36.0
36	34.286125301409314	36.0	36.0	36.0	32.0	36.0
37	34.28047993033686	36.0	36.0	36.0	32.0	36.0
38	34.233225776100156	36.0	36.0	36.0	32.0	36.0
39	34.21401491042124	36.0	36.0	36.0	32.0	36.0
40	34.19560196075176	36.0	36.0	36.0	32.0	36.0
41	34.17070329147272	36.0	36.0	36.0	32.0	36.0
42	34.09880875290801	36.0	36.0	36.0	32.0	36.0
43	34.09605801817957	36.0	36.0	36.0	32.0	36.0
44	34.015735088195086	36.0	36.0	36.0	32.0	36.0
45	33.97641028195492	36.0	36.0	36.0	32.0	36.0
46	33.95361663502676	36.0	36.0	36.0	32.0	36.0
47	33.93029627128775	36.0	36.0	36.0	32.0	36.0
48	33.86489759924138	36.0	36.0	36.0	32.0	36.0
49	33.854293157109204	36.0	36.0	36.0	32.0	36.0
50	33.14683683955155	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	5.0
21	33.0
22	93.0
23	322.0
24	878.0
25	1967.0
26	4132.0
27	7907.0
28	13582.0
29	21947.0
30	33615.0
31	50097.0
32	75723.0
33	125084.0
34	244193.0
35	504492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.073420351275985	18.636778349635744	12.094380857405643	26.195420441682632
2	15.659214202759783	20.663314487704216	37.48407622747957	26.19339508205643
3	18.297619157434482	24.55570212255666	28.920549411015894	28.226129308992963
4	12.02362569344897	15.995402527871713	36.51088394334704	35.47008783533228
5	14.107431780487309	37.09838996230885	33.4109110632669	15.383267193936945
6	34.34968461562587	36.248355739552835	16.32259421404812	13.079365430773176
7	30.062920117003188	30.536504460492974	20.7606867803183	18.639888642185536
8	27.72273848464219	34.0090252141459	19.093530515444517	19.174705785767394
9	27.127507467285085	13.835832267896118	18.360019998856153	40.67664026596265
10	15.363959474679978	26.965499237590436	31.876652925152875	25.793888362576713
11	37.4856328995991	20.696004147318355	22.827131727169917	18.99123122591262
12	24.739708008054823	23.4793662038741	29.229081859029527	22.55184392904155
13	29.51368454066619	19.38158974973941	25.589675943435385	25.51504976615901
14	23.101098264509616	20.103332429336003	25.198648800727625	31.596920505426752
15	24.69403324468208	28.268219401248867	22.07388588618507	24.963861467883984
16	25.308675570790367	25.94959536633806	24.338717071915298	24.403011990956276
17	23.39231454679293	26.53914769655946	25.51753847062101	24.550999286026602
18	24.416666436054467	25.06468672254862	26.91558025258493	23.603066588811988
19	25.623920160358203	24.898507757318928	25.360746001422413	24.116826080900456
20	25.642281597505683	24.77065489615475	25.16140637323408	24.42565713310549
21	26.46156996662589	24.342434188072033	24.518344688646714	24.677651156655358
22	25.514056710203093	24.94942683749157	25.039273185477363	24.497243266827972
23	23.943955596922336	24.634317557277257	25.83663954969656	25.585087296103847
24	24.686274292756544	25.25247493699657	25.537420161834866	24.523830608412016
25	24.632535399658686	24.930953369309535	25.75185646418523	24.68465476684655
26	24.120533943413804	25.780389479801848	26.164887777788024	23.93418879899632
27	24.7677367397074	25.297530366501324	25.286644827939302	24.648088065851972
28	23.888031999291528	25.12942517877833	25.95588289558165	25.026659926348493
29	24.195076412738562	24.997716888658886	25.734216320879078	25.072990377723475
30	23.9161017207136	25.451580080569947	26.032930914082396	24.599387284634055
31	24.972578135017976	24.85800132658173	25.10071596667494	25.06870457172536
32	24.45048153204679	25.391682963728275	24.894468838788235	25.2633666654367
33	23.92434011156734	25.08595408340706	25.88548619061281	25.10421961441279
34	24.8425804809021	25.171533311317045	25.879898085505598	24.105988122275253
35	25.239198698198216	25.171581017604584	25.738259614994334	23.850960669202866
36	24.026468830028268	25.757846921379496	25.420851814404532	24.794832434187704
37	25.025091417628197	25.398326254847625	25.01752709319617	24.55905523432801
38	24.33732939108936	25.417510389903917	25.45247397334834	24.79268624565838
39	25.194000110699065	24.82777070533754	24.986162616925885	24.992066567037508
40	25.484829448332675	25.24037374970135	25.45595600166414	23.81884080030183
41	24.30133197789354	25.324184729716144	26.18892333862533	24.18555995376499
42	25.432149785481762	25.952602123320084	25.22099852683023	23.394249564367925
43	24.351671544946438	25.208360814975595	25.69569681299046	24.744270827087504
44	24.14902920144347	25.741474610167742	25.1168758809508	24.992620307437992
45	24.30739343041925	25.941993825047945	25.149969051442866	24.60064369308994
46	24.32097603813825	25.213365361199052	25.200912130000663	25.26474647066203
47	24.838568033887796	25.15894130149218	25.583645742623943	24.418844921996076
48	25.382475981384705	25.756626739418202	24.474311727725993	24.386585551471097
49	24.606666740462778	25.63040325883616	24.842629798130744	24.92030020257032
50	24.58088190527848	26.073600956402863	24.525719008691386	24.819798129627273
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	12.5
2	11.0
3	48.5
4	86.0
5	105.0
6	124.0
7	132.0
8	140.0
9	205.0
10	270.0
11	391.5
12	513.0
13	894.5
14	1276.0
15	1787.5
16	2299.0
17	2546.5
18	2794.0
19	2923.5
20	3053.0
21	3602.5
22	4152.0
23	4881.5
24	5611.0
25	6519.5
26	7428.0
27	9608.5
28	11789.0
29	13926.0
30	16063.0
31	18324.5
32	20586.0
33	24422.0
34	28258.0
35	32775.0
36	37292.0
37	42220.0
38	47148.0
39	49212.5
40	51277.0
41	54411.0
42	57545.0
43	57708.5
44	57872.0
45	63154.0
46	68436.0
47	73022.0
48	77608.0
49	79454.5
50	81301.0
51	78703.0
52	76105.0
53	74495.5
54	72886.0
55	73589.0
56	74292.0
57	72469.0
58	70646.0
59	63924.5
60	57203.0
61	50727.0
62	44251.0
63	38284.5
64	32318.0
65	27419.0
66	22520.0
67	19257.0
68	15994.0
69	14551.0
70	13108.0
71	10258.5
72	7409.0
73	6320.5
74	5232.0
75	4119.5
76	3007.0
77	2664.0
78	2321.0
79	1900.5
80	1480.0
81	1248.0
82	1016.0
83	823.0
84	630.0
85	463.5
86	297.0
87	233.5
88	170.0
89	127.5
90	85.0
91	73.5
92	62.0
93	46.0
94	30.0
95	28.5
96	27.0
97	22.0
98	17.0
99	19.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0076563039054529484
2	2.76733876100709E-4
3	3.6897850146761196E-4
4	0.0
5	0.0
6	0.0
7	9.224462536690299E-5
8	0.0
9	0.001106935504402836
10	0.001014690879035933
11	0.0
12	2.76733876100709E-4
13	3.6897850146761196E-4
14	0.0
15	0.004704475893712053
16	6.45712377568321E-4
17	0.0
18	2.76733876100709E-4
19	9.224462536690299E-5
20	8.30201628302127E-4
21	0.0
22	9.224462536690299E-5
23	4.61223126834515E-4
24	5.53467752201418E-4
25	0.002213871008805672
26	0.005903656023481791
27	0.006180389899582501
28	0.00461223126834515
29	0.002490604884906381
30	0.004335497392244441
31	0.007840793156186755
32	0.003136317262474702
33	0.005995900648848695
34	0.002582849510273284
35	0.0035052957639423138
36	0.007564059280086046
37	0.0035052957639423138
38	0.008209771657654366
39	0.004981209769812762
40	0.003044072637107799
41	0.004335497392244441
42	0.001568158631237351
43	0.001568158631237351
44	0.001660403256604254
45	0.001568158631237351
46	0.001660403256604254
47	0.002398360259539478
48	0.0017526478819711569
49	5.53467752201418E-4
50	0.001106935504402836
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1084074.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.708447736031346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.5125143972823	47.267647435893196
2	11.92128840686661	13.997606747613695
3	3.419909549559188	6.023327431567503
4	1.4093036597670492	3.30952121014526
5	0.7268670608874387	2.1336618427576455
6	0.4391581065308424	1.5469374447072266
7	0.284757743171506	1.1702379557690217
8	0.20300082316255147	0.9534290573607995
9	0.15482146802212468	0.8180395257413299
>10	0.7779039060109981	8.303098868650922
>50	0.07106809097859661	2.873679346703739
>100	0.07214493591623977	8.44879009479189
>500	0.005841054744621376	2.226658149778269
>1k	0.0014207971000430373	0.9273648885195154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1267	0.1168739403398661	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1184	0.10921763643441315	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1134	0.10460540516606799	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1118	0.10312949116019754	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1114	0.10276051265872994	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1102	0.1016535771543271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	9.2244625366903E-5
6	0.0	0.0	0.0	0.0	2.76733876100709E-4
7	0.0	0.0	0.0	9.2244625366903E-5	2.76733876100709E-4
8	0.0	0.0	0.0	9.2244625366903E-5	2.76733876100709E-4
9	0.0	0.0	0.0	9.2244625366903E-5	2.76733876100709E-4
10	0.0	0.0	0.0	1.84489250733806E-4	2.76733876100709E-4
11	0.0	0.0	0.0	1.84489250733806E-4	2.76733876100709E-4
12	0.0	0.0	0.0	1.84489250733806E-4	3.68978501467612E-4
13	0.0	0.0	0.0	1.84489250733806E-4	3.68978501467612E-4
14	0.0	0.0	0.0	1.84489250733806E-4	3.68978501467612E-4
15	0.0	0.0	0.0	1.84489250733806E-4	3.68978501467612E-4
16	0.0	0.0	0.0	2.76733876100709E-4	3.68978501467612E-4
17	0.0	0.0	0.0	2.76733876100709E-4	3.68978501467612E-4
18	0.0	0.0	0.0	2.76733876100709E-4	3.68978501467612E-4
19	0.0	0.0	0.0	2.76733876100709E-4	3.68978501467612E-4
20	0.0	0.0	0.0	2.76733876100709E-4	3.68978501467612E-4
21	0.0	0.0	0.0	4.61223126834515E-4	3.68978501467612E-4
22	0.0	0.0	0.0	7.37957002935224E-4	4.61223126834515E-4
23	0.0	0.0	0.0	8.302016283021269E-4	4.61223126834515E-4
24	0.0	0.0	0.0	9.2244625366903E-4	4.61223126834515E-4
25	0.0	0.0	0.0	9.2244625366903E-4	4.61223126834515E-4
26	0.0	0.0	0.0	0.001199180129769739	4.61223126834515E-4
27	0.0	0.0	0.0	0.0015681586312373509	5.53467752201418E-4
28	0.0	0.0	0.0	0.003044072637107799	5.53467752201418E-4
29	0.0	0.0	0.0	0.005995900648848695	5.53467752201418E-4
30	0.0	0.0	0.0	0.010331398041093136	5.53467752201418E-4
31	0.0	0.0	0.0	0.019186882076315824	5.53467752201418E-4
32	0.0	0.0	0.0	0.02951828011740896	5.53467752201418E-4
33	9.2244625366903E-5	0.0	0.0	0.04012641203460281	5.53467752201418E-4
34	9.2244625366903E-5	0.0	0.0	0.053132904211336124	6.45712377568321E-4
35	9.2244625366903E-5	0.0	0.0	0.06853775664760893	6.45712377568321E-4
36	9.2244625366903E-5	0.0	0.0	0.09104544523713326	6.45712377568321E-4
37	9.2244625366903E-5	0.0	0.0	0.12397677649311763	6.45712377568321E-4
38	9.2244625366903E-5	0.0	0.0	0.16557910253359087	6.45712377568321E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	385	0.0	21.717566	36
ATACGAA	375	0.0	21.707941	40
CGTCGTA	275	0.0	20.797384	10
CTAGCGG	415	0.0	20.145761	29
ACTGTTC	415	0.0	20.141117	8
CCCTTAC	110	1.267017E-7	19.997484	1
ACTATAC	110	1.2676355E-7	19.996563	3
TCTAGCG	420	0.0	19.90593	28
GATATAC	300	0.0	19.79751	1
TAGCGGC	425	0.0	19.67265	30
CGAATGC	405	0.0	19.555801	43
CAATACG	430	0.0	19.444796	38
GCAATAC	435	0.0	19.221294	37
GCGCAAT	440	0.0	19.002869	35
ACCGTCG	290	0.0	18.962257	8
AATACGA	445	0.0	18.788486	39
GTCGTAG	305	0.0	18.75174	11
TACGAAT	435	0.0	18.714605	41
GTCCTAC	720	0.0	18.636545	1
GTATACC	60	0.007418974	18.330181	4
>>END_MODULE