FastQCFastQC Report
Thu 2 Feb 2017
SRR4063952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465340
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT39500.8488417071388662No Hit
GTCCTACAGTGGACATTTCTAAATT35610.7652469162332918No Hit
CTGTAGGACGTGGAATATGGCAAGA34430.7398891133364851No Hit
CTTTAGGACGTGAAATATGGCGAGG31550.6779988825374995No Hit
GTATCAACGCAGAGTACTTTTTTTT30090.6466239738685692No Hit
GTCCTACAGTGTGCATTTCTCATTT26080.5604504233463704No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24070.5172561997679116No Hit
TATCAACGCAGAGTACTTTTTTTTT22940.4929728800446985No Hit
CTGTAGGACCTGGAATATGGCGAGA19290.41453560837237285No Hit
CTGAAGGACCTGGAATATGGCGAGA16510.3547943439205742No Hit
GATATACACTGTTCTACAAATCCCG16330.3509262044956376No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15760.3386770963166717No Hit
ATTTAGAAATGTCCACTGTAGGACG15760.3386770963166717No Hit
GTACATGGGGTGGTATCAACGCAAA15650.336313233334766No Hit
GTCCTTCAGTGTGCATTTCTCATTT14320.30773198091717885No Hit
TTGTAGAACAGTGTATATCAATGAG14100.3030042549533674No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13780.296127562642369No Hit
TTTCTAAATTTTCCACCTTTTTCAG12160.2613143078179396No Hit
GTATCAACGCAGAGTACATGGGGTG11590.24906519963897367No Hit
GGTATCAACGCAGAGTACTTTTTTT11080.23810547126831993No Hit
GAATATGGCAAGAAAACTGAAAATC11010.23660119482528902No Hit
GTGTATATCAATGAGTTACAATGAA10820.23251815876563373No Hit
GGAATATGGCGAGAAAACTGAAAAT9380.20157304336614087No Hit
GATATACACTGTTCTACAATGCCGG8890.19104310826492457No Hit
GTGTATATCAATGAGTTACAATGAG8850.19018352172604977No Hit
GTTCTACAGTGTGGTTTTTATCATT8560.18395151931920747No Hit
GAAATATGGCGAGGAAAACTGAAAA7970.17127261787080414No Hit
GCCATATTCCACGTCCTACAGTGGA7870.16912365152361714No Hit
GTACATGGGTGGTATCAACGCAAAA7810.16783427171530493No Hit
GTTCTACAAATCCCGTTTCCAACGA7650.16439592555980576No Hit
ATTCCAGGTCCTTCAGTGTGCATTT7480.16074268276958784No Hit
CTGTAGGACATGGAATATGGCAAGA7360.1581639231529634No Hit
CCATATTCCAGGTCCTTCAGTGTGC7240.15558516353633903No Hit
CTGTAGAACATATTAGATGAGTGAG6840.146989298147591No Hit
TATCAACGCAGAGTACATGGGGTGG6760.1452701250698414No Hit
ACAGTGGACATTTCTAAATTTTCCA6700.1439807452615292No Hit
TTCCAGGTCCTTCAGTGTGCATTTC6690.1437658486268105No Hit
GACCTGGAATATGGCGAGAAAACTG6600.1418317789143422No Hit
CCATATTTCACGTCCTAAAGTGTGT6580.1414019856449048No Hit
ACCTGGAATATGGCGAGAAAACTGA6480.1392530192977178No Hit
GTCCACTGTAGGACGTGGAATATGG6290.1351699832380625No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6250.1343103966991877No Hit
ATACACACTTTAGGACGTGAAATAT6170.13259122362143808No Hit
CCACTGTAGGACGTGGAATATGGCA6080.1306571539089698No Hit
GTGTATTTCTCATTTTCCGTGATTT5920.12721880775347058No Hit
GGTATCAACGCAGAGTACATGGGGT5890.1265741178493145No Hit
CCTAAAGTGTGTATTTCTCATTTTC5640.12120170198134698No Hit
GTAGAACATATTAGATGAGTGAGTT5580.11991232217303477No Hit
CACTTTAGGACGTGAAATATGGCGA5400.11604418274809816No Hit
TCCTAAAGTGTGTATTTCTCATTTT5240.11260583659259897No Hit
GAAATACACACTTTAGGACGTGAAA5190.11153135341900546No Hit
GTACATGGGAGTGGTATCAACGCAA5190.11153135341900546No Hit
ATGTTCTACAGTGTGGTTTTTATCA5110.10981218034125585No Hit
GTAGGACGTGGAATATGGCAAGAAA4900.10529935101216314No Hit
TTGATATACACTGTTCTACAAATCC4870.10465466110800704No Hit
AGTGTGTATTTCTCATTTTCCGTGA4680.10057162504835174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCAA250.006022705719.0017217
CGGCAAG250.0060264518.99967618
TTTAACC402.7562538E-416.6247184
CCAACGA1950.016.56382219
CTAGGTC350.002167369716.2854394
CGGTAGG350.002172047316.2801841
TCCAACG2750.015.8906418
GTCCTAG1200.015.0365591
TCTAGGA1151.8189894E-1214.8629172
ACTGTGC456.745087E-414.7791178
CACAGCC456.745087E-414.7791179
AGTGTCC456.745087E-414.7791178
AGTACCT602.5584413E-514.251295
TTAAACC400.005276114.2482253
CTCTAGG954.8912625E-913.9952451
GGCCTAA551.9540006E-413.81943417
GTAGTAC551.9575712E-413.8164613
GTGTAGG1051.366061E-913.566821
CCAACGT852.6858288E-713.41153719
GTTTAGG852.6964153E-713.407211
Produced by FastQC (version 0.11.5)