##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063952_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465340
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21634074010401	32.0	32.0	32.0	32.0	32.0
2	31.006023552671166	32.0	32.0	32.0	32.0	32.0
3	31.03412343662698	32.0	32.0	32.0	32.0	32.0
4	31.0209717625822	32.0	32.0	32.0	32.0	32.0
5	31.062668156616667	32.0	32.0	32.0	32.0	32.0
6	34.698764344350366	36.0	36.0	36.0	32.0	36.0
7	34.57736493746508	36.0	36.0	36.0	32.0	36.0
8	34.52939356169682	36.0	36.0	36.0	32.0	36.0
9	34.543204968410194	36.0	36.0	36.0	32.0	36.0
10	34.40379292560279	36.0	36.0	36.0	32.0	36.0
11	34.60946834572571	36.0	36.0	36.0	32.0	36.0
12	34.47739287402759	36.0	36.0	36.0	32.0	36.0
13	34.501063738341855	36.0	36.0	36.0	32.0	36.0
14	34.5030192977178	36.0	36.0	36.0	32.0	36.0
15	34.472218163063566	36.0	36.0	36.0	32.0	36.0
16	34.45787166372975	36.0	36.0	36.0	32.0	36.0
17	34.43146946318821	36.0	36.0	36.0	32.0	36.0
18	34.437359779945844	36.0	36.0	36.0	32.0	36.0
19	34.333917135857654	36.0	36.0	36.0	32.0	36.0
20	34.27466153780032	36.0	36.0	36.0	32.0	36.0
21	34.2444986461512	36.0	36.0	36.0	32.0	36.0
22	34.228742424893625	36.0	36.0	36.0	32.0	36.0
23	34.2742919155886	36.0	36.0	36.0	32.0	36.0
24	34.215253363132334	36.0	36.0	36.0	32.0	36.0
25	33.93292861133795	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	3.0
6	13.0
7	5.0
8	38.0
9	25.0
10	45.0
11	12.0
12	30.0
13	21.0
14	69.0
15	84.0
16	136.0
17	184.0
18	234.0
19	300.0
20	483.0
21	657.0
22	953.0
23	1449.0
24	2083.0
25	3089.0
26	4346.0
27	5869.0
28	7951.0
29	10875.0
30	14329.0
31	19745.0
32	28071.0
33	40446.0
34	92438.0
35	231353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.965647862101285	19.750490698759332	12.704421985884215	24.579439453255166
2	11.25975689429888	20.834407881163607	49.282846778075786	18.62298844646173
3	19.708037040141146	27.38340070444176	30.069863946893943	22.838698308523146
4	10.065867941761136	17.88728308171708	40.374576908612255	31.672272067909525
5	9.628266929145704	41.87257015260892	36.90445232932236	11.59471058892301
6	27.96270664798274	40.40564472451234	19.757180560689815	11.874468066815107
7	25.444556453779672	32.53016191677826	22.780119465479036	19.24516216396304
8	21.990407828518972	43.6458850616225	20.04445676966105	14.319250340197476
9	27.170503385213422	14.505569638215393	21.064219078886545	37.25970789768464
10	15.489608373505115	29.34704549266157	35.60717802728958	19.556168106543737
11	33.14835519243174	20.522898301440552	29.864975274134594	16.463771231993118
12	26.464636907667398	23.941666111167258	34.87453956266786	14.719157418497486
13	31.332708294806018	23.376369242943586	26.904898094349278	18.38602436790112
14	18.64909036170487	26.399294653993376	28.382005075050536	26.569609909251213
15	19.42343171448108	39.599236230868144	24.04733132356546	16.930000731085318
16	17.064265163146555	27.990590097238528	36.32188060433251	18.623264135282405
17	16.96570647078556	31.263923413698492	32.33629506025236	19.434075055263587
18	16.786853373651258	27.527738289054586	39.659677700329695	16.025730636964454
19	24.015423755422077	24.741880055140143	28.887283143762215	22.35541304567556
20	23.579624272794728	29.16907724237308	31.295258782919145	15.956039701913047
21	21.325880827288387	25.97394777080748	27.892057661579088	24.80811374032504
22	22.458648670154997	32.761909677641356	28.32450497764415	16.454936674559494
23	18.880634573774792	32.240365464939714	31.369504153206208	17.509495808079286
24	23.403738249418787	27.649109525847514	32.52870572092813	16.418446503805566
25	20.270206332342607	30.42036397420953	32.93682711870239	16.37260257474547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	187.0
1	187.0
2	432.0
3	677.0
4	677.0
5	677.0
6	1798.0
7	2919.0
8	2919.0
9	2919.0
10	2661.5
11	2404.0
12	2404.0
13	2404.0
14	2037.0
15	1670.0
16	1670.0
17	1670.0
18	3334.0
19	4998.0
20	4998.0
21	4998.0
22	9764.0
23	14530.0
24	14530.0
25	14530.0
26	24795.5
27	35061.0
28	35061.0
29	35061.0
30	48850.5
31	62640.0
32	62640.0
33	62640.0
34	63146.0
35	63652.0
36	63652.0
37	63652.0
38	61652.5
39	59653.0
40	59653.0
41	59653.0
42	58751.0
43	57849.0
44	57849.0
45	57849.0
46	58952.5
47	60056.0
48	60056.0
49	60056.0
50	53320.5
51	46585.0
52	46585.0
53	46585.0
54	34641.0
55	22697.0
56	22697.0
57	22697.0
58	18073.5
59	13450.0
60	13450.0
61	13450.0
62	10554.5
63	7659.0
64	7659.0
65	7659.0
66	6009.5
67	4360.0
68	4360.0
69	4360.0
70	3281.5
71	2203.0
72	2203.0
73	2203.0
74	1607.0
75	1011.0
76	1011.0
77	1011.0
78	787.5
79	564.0
80	564.0
81	564.0
82	369.0
83	174.0
84	174.0
85	174.0
86	127.5
87	81.0
88	81.0
89	81.0
90	54.5
91	28.0
92	28.0
93	28.0
94	29.5
95	31.0
96	31.0
97	31.0
98	116.0
99	201.0
100	201.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04018567069239696
2	0.006017105772123609
3	0.0015042764430309022
4	0.0032234495207805044
5	0.007091588945717111
6	0.012034211544247218
7	0.02170456010658873
8	0.03631753126746035
9	0.04921132935058237
10	0.054583745218549876
11	0.05157519233248807
12	0.06296471397257919
13	0.06489878368504749
14	0.0687669231099841
15	0.05974126445179868
16	0.06403919714617269
17	0.06360940387673529
18	0.07929685821120042
19	0.07370954570851421
20	0.0805862380195126
21	0.07542871878626381
22	0.07757768513345081
23	0.08552886061804273
24	0.07800747840288821
25	0.07779258176816951
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	465340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.47033173872548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12209789147015	29.997458908380814
2	14.304448579188358	11.578115586785865
3	4.805573631536187	5.834494771894237
4	2.1055436638096126	3.40848202258986
5	1.0993431474528776	2.224539093605628
6	0.672254226222847	1.6323810928799296
7	0.45713736880230954	1.2950350675918294
8	0.3350823994326825	1.0848716691879023
9	0.23744713339955648	0.8648607833180508
>10	1.5056583957234033	11.680188499074815
>50	0.1856876249607111	5.224643910283529
>100	0.1415269577064433	11.133805747981729
>500	0.016493743191352415	4.514502317136653
>1k	0.011705237103540423	9.526620529289179
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	3950	0.8488417071388662	No Hit
GTCCTACAGTGGACATTTCTAAATT	3561	0.7652469162332918	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3443	0.7398891133364851	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3155	0.6779988825374995	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3009	0.6466239738685692	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2608	0.5604504233463704	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2407	0.5172561997679116	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2294	0.4929728800446985	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1929	0.41453560837237285	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1651	0.3547943439205742	No Hit
GATATACACTGTTCTACAAATCCCG	1633	0.3509262044956376	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1576	0.3386770963166717	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1576	0.3386770963166717	No Hit
GTACATGGGGTGGTATCAACGCAAA	1565	0.336313233334766	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1432	0.30773198091717885	No Hit
TTGTAGAACAGTGTATATCAATGAG	1410	0.3030042549533674	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1378	0.296127562642369	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1216	0.2613143078179396	No Hit
GTATCAACGCAGAGTACATGGGGTG	1159	0.24906519963897367	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1108	0.23810547126831993	No Hit
GAATATGGCAAGAAAACTGAAAATC	1101	0.23660119482528902	No Hit
GTGTATATCAATGAGTTACAATGAA	1082	0.23251815876563373	No Hit
GGAATATGGCGAGAAAACTGAAAAT	938	0.20157304336614087	No Hit
GATATACACTGTTCTACAATGCCGG	889	0.19104310826492457	No Hit
GTGTATATCAATGAGTTACAATGAG	885	0.19018352172604977	No Hit
GTTCTACAGTGTGGTTTTTATCATT	856	0.18395151931920747	No Hit
GAAATATGGCGAGGAAAACTGAAAA	797	0.17127261787080414	No Hit
GCCATATTCCACGTCCTACAGTGGA	787	0.16912365152361714	No Hit
GTACATGGGTGGTATCAACGCAAAA	781	0.16783427171530493	No Hit
GTTCTACAAATCCCGTTTCCAACGA	765	0.16439592555980576	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	748	0.16074268276958784	No Hit
CTGTAGGACATGGAATATGGCAAGA	736	0.1581639231529634	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	724	0.15558516353633903	No Hit
CTGTAGAACATATTAGATGAGTGAG	684	0.146989298147591	No Hit
TATCAACGCAGAGTACATGGGGTGG	676	0.1452701250698414	No Hit
ACAGTGGACATTTCTAAATTTTCCA	670	0.1439807452615292	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	669	0.1437658486268105	No Hit
GACCTGGAATATGGCGAGAAAACTG	660	0.1418317789143422	No Hit
CCATATTTCACGTCCTAAAGTGTGT	658	0.1414019856449048	No Hit
ACCTGGAATATGGCGAGAAAACTGA	648	0.1392530192977178	No Hit
GTCCACTGTAGGACGTGGAATATGG	629	0.1351699832380625	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	625	0.1343103966991877	No Hit
ATACACACTTTAGGACGTGAAATAT	617	0.13259122362143808	No Hit
CCACTGTAGGACGTGGAATATGGCA	608	0.1306571539089698	No Hit
GTGTATTTCTCATTTTCCGTGATTT	592	0.12721880775347058	No Hit
GGTATCAACGCAGAGTACATGGGGT	589	0.1265741178493145	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	564	0.12120170198134698	No Hit
GTAGAACATATTAGATGAGTGAGTT	558	0.11991232217303477	No Hit
CACTTTAGGACGTGAAATATGGCGA	540	0.11604418274809816	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	524	0.11260583659259897	No Hit
GAAATACACACTTTAGGACGTGAAA	519	0.11153135341900546	No Hit
GTACATGGGAGTGGTATCAACGCAA	519	0.11153135341900546	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	511	0.10981218034125585	No Hit
GTAGGACGTGGAATATGGCAAGAAA	490	0.10529935101216314	No Hit
TTGATATACACTGTTCTACAAATCC	487	0.10465466110800704	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	468	0.10057162504835174	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGCAA	25	0.0060227057	19.00172	17
CGGCAAG	25	0.00602645	18.999676	18
TTTAACC	40	2.7562538E-4	16.624718	4
CCAACGA	195	0.0	16.563822	19
CTAGGTC	35	0.0021673697	16.285439	4
CGGTAGG	35	0.0021720473	16.280184	1
TCCAACG	275	0.0	15.89064	18
GTCCTAG	120	0.0	15.036559	1
TCTAGGA	115	1.8189894E-12	14.862917	2
ACTGTGC	45	6.745087E-4	14.779117	8
CACAGCC	45	6.745087E-4	14.779117	9
AGTGTCC	45	6.745087E-4	14.779117	8
AGTACCT	60	2.5584413E-5	14.25129	5
TTAAACC	40	0.0052761	14.248225	3
CTCTAGG	95	4.8912625E-9	13.995245	1
GGCCTAA	55	1.9540006E-4	13.819434	17
GTAGTAC	55	1.9575712E-4	13.816461	3
GTGTAGG	105	1.366061E-9	13.56682	1
CCAACGT	85	2.6858288E-7	13.411537	19
GTTTAGG	85	2.6964153E-7	13.40721	1
>>END_MODULE