FastQCFastQC Report
Thu 2 Feb 2017
SRR4063952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465340
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA17700.38036704345209954No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA15020.32277474534748785No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA13480.289680663600808No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC13160.2828039712898096No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11120.2389650578071947No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG10100.2170456010658873No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9560.2054411827910775No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA8470.18201744960673916No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA8040.17277689431383503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.16224695921261872No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA6630.1424764688184983No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6620.1422615721837796No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA6150.1321614303520007No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC6110.1313018438131259No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT5880.12635922121459578No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA5600.12034211544247218No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5430.11668887265225426No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC5250.11282073322731766No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4720.10143121158722654No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG4700.10100141831778915No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4660.10014183177891434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT207.84343E-444.0093525
CGAGATA300.005738481429.33641221
CACGGCA300.00574149729.33326137
GGACCGT300.00574753229.3269586
TAGGACC7500.029.3269584
ATACGGC454.0702616E-529.32695615
TTACTCG407.0212747E-427.5028943
GTATAGT407.030104E-427.4969771
GATCTAC407.030104E-427.4969771
AATGGCG655.929269E-727.0710416
TGACGAG508.336839E-526.39709918
TATGACG508.342937E-526.3942616
GTAAGAC508.342937E-526.394263
GTATAGA450.001397817924.4417571
TAGCACC450.001398693424.439134
AGGACCT14500.023.9672035
GCGGTAT1202.3646862E-1123.83583644
TAGGACA4800.023.8281544
GACGAGA651.8088105E-523.69479819
TCGAGGA852.3438224E-723.29656620
Produced by FastQC (version 0.11.5)