FastQCFastQC Report
Thu 2 Feb 2017
SRR4063951_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063951_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877818
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26110.29744206657872135No Hit
TATCAACGCAGAGTACTTTTTTTTT18070.20585132681261945No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13300.15151204463795456No Hit
GGTATCAACGCAGAGTACTTTTTTT9460.107767213704891No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8950.10195735334659348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG456.768947E-414.7746745
CGCATCG653.3654578E-614.61648113
TAAGACC653.3754277E-614.6123154
TCCAACG602.559798E-514.25350618
ACCCGGT707.248298E-613.57244812
CTAGCAT707.269673E-613.568584
CGAGCCG1650.013.24492715
CGCCGGT751.4774379E-512.6661727
CGCGGTT1001.4392936E-712.35022210
AGGCCCG1850.012.32454710
GCATCGC853.929468E-612.29644314
CCCGTTT701.08909255E-412.21520312
ACCTCCG1103.788955E-812.09457519
ATCGCCA1103.7949576E-812.09319516
ATCCCGT550.003066199112.09112710
ATTATAC550.003075376412.0863043
GCAACTT951.0356798E-612.00226914
TCCCGGA951.0356798E-612.00226917
CAACCCG802.8624987E-511.87521410
GTGTTAT802.8641694E-511.87453651
Produced by FastQC (version 0.11.5)