##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4063951_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 877818 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24379313251722 32.0 32.0 32.0 32.0 32.0 2 30.86716836519643 32.0 32.0 32.0 32.0 32.0 3 30.892277214639027 32.0 32.0 32.0 32.0 32.0 4 30.92745193194945 32.0 32.0 32.0 32.0 32.0 5 30.843551852434103 32.0 32.0 32.0 32.0 32.0 6 34.481245542925755 36.0 36.0 36.0 32.0 36.0 7 34.430872914431006 36.0 36.0 36.0 32.0 36.0 8 34.38152213784634 36.0 36.0 36.0 32.0 36.0 9 34.51210729331137 36.0 36.0 36.0 32.0 36.0 10 34.24056125529438 36.0 36.0 36.0 32.0 36.0 11 34.49382787776054 36.0 36.0 36.0 32.0 36.0 12 34.326116575417686 36.0 36.0 36.0 32.0 36.0 13 34.40339797087779 36.0 36.0 36.0 32.0 36.0 14 34.31379739308148 36.0 36.0 36.0 32.0 36.0 15 34.25887826405929 36.0 36.0 36.0 32.0 36.0 16 34.25461086466671 36.0 36.0 36.0 32.0 36.0 17 34.18759925178112 36.0 36.0 36.0 32.0 36.0 18 34.1826255556391 36.0 36.0 36.0 32.0 36.0 19 34.19306849483606 36.0 36.0 36.0 32.0 36.0 20 34.16057998355012 36.0 36.0 36.0 32.0 36.0 21 34.15391003602113 36.0 36.0 36.0 32.0 36.0 22 34.13296378064701 36.0 36.0 36.0 32.0 36.0 23 34.088806563547344 36.0 36.0 36.0 32.0 36.0 24 34.076278909751224 36.0 36.0 36.0 32.0 36.0 25 33.66311125996505 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 7.0 5 7.0 6 36.0 7 11.0 8 47.0 9 59.0 10 92.0 11 22.0 12 53.0 13 59.0 14 95.0 15 171.0 16 278.0 17 367.0 18 489.0 19 590.0 20 933.0 21 1326.0 22 1915.0 23 3038.0 24 4544.0 25 6576.0 26 9219.0 27 12406.0 28 17036.0 29 22861.0 30 30363.0 31 41939.0 32 58431.0 33 83653.0 34 180610.0 35 400585.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.44205049889167 17.218629087863057 11.355469573591508 25.983850839653766 2 16.411532366568576 20.00198228927069 38.02475127396263 25.561734070198106 3 18.858196134900275 24.19936568983509 28.847897708372255 28.094540466892383 4 12.450144624733278 15.573674311252475 36.59546884274011 35.38071222127414 5 14.257964479271221 36.90405256220229 33.96571425967341 14.872268698853079 6 33.368234563611196 35.96543297083905 17.34583549329794 13.320496972251814 7 29.637989715448594 30.710199207636045 21.118197138419852 18.53361393849551 8 27.64552570909559 33.21093891561281 19.823907025827044 19.31962834946455 9 27.328306222189802 14.1875319133416 18.743047450579912 39.74111441388868 10 15.401152419111236 26.945861379141327 32.308998786027345 25.343987415720093 11 36.650427892090036 21.329064290957504 23.01384665186408 19.00666116508838 12 24.345930812469575 23.806625104447022 29.595811403733762 22.25163267934964 13 29.354547848359836 19.744750992008083 25.655420678680574 25.24528048095151 14 23.276513618606074 19.648913213234096 26.008214596355074 31.06635857180476 15 24.815825079705593 27.501137022949056 22.794374956542356 24.888662940802995 16 25.09615625762353 25.940416179330565 24.430757234803664 24.53267032824224 17 23.597485767960187 25.93366056955518 25.842124102019277 24.626729560465364 18 24.01825484502421 25.536603746051075 26.75511351134429 23.690027897580425 19 25.07501447847837 24.997605920899627 26.0331021336945 23.89427746692751 20 25.447201187520097 24.596692364346122 25.57477751099619 24.381328937137592 21 26.213733913408305 24.46609844857307 25.09052270592205 24.22964493209657 22 25.324098617642864 24.679948696023942 25.586318939717827 24.409633746615363 23 24.21782451403703 24.516431357481075 25.916884911518196 25.3488592169637 24 24.225408957619816 25.122789895885113 25.93432051697168 24.71748062952339 25 24.456833404588856 24.80718255664814 26.041670229442783 24.694313809320224 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 143.0 1 143.0 2 283.0 3 423.0 4 423.0 5 423.0 6 1129.0 7 1835.0 8 1835.0 9 1835.0 10 1918.0 11 2001.0 12 2001.0 13 2001.0 14 2266.5 15 2532.0 16 2532.0 17 2532.0 18 4557.0 19 6582.0 20 6582.0 21 6582.0 22 10322.0 23 14062.0 24 14062.0 25 14062.0 26 20533.0 27 27004.0 28 27004.0 29 27004.0 30 34396.0 31 41788.0 32 41788.0 33 41788.0 34 50253.0 35 58718.0 36 58718.0 37 58718.0 38 67147.0 39 75576.0 40 75576.0 41 75576.0 42 85211.0 43 94846.0 44 94846.0 45 94846.0 46 104734.0 47 114622.0 48 114622.0 49 114622.0 50 117369.0 51 120116.0 52 120116.0 53 120116.0 54 109935.0 55 99754.0 56 99754.0 57 99754.0 58 90451.0 59 81148.0 60 81148.0 61 81148.0 62 70600.0 63 60052.0 64 60052.0 65 60052.0 66 48994.5 67 37937.0 68 37937.0 69 37937.0 70 28410.5 71 18884.0 72 18884.0 73 18884.0 74 14400.5 75 9917.0 76 9917.0 77 9917.0 78 7708.5 79 5500.0 80 5500.0 81 5500.0 82 3822.5 83 2145.0 84 2145.0 85 2145.0 86 1653.5 87 1162.0 88 1162.0 89 1162.0 90 812.5 91 463.0 92 463.0 93 463.0 94 310.0 95 157.0 96 157.0 97 157.0 98 304.0 99 451.0 100 451.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04249172379696019 2 0.005126347374968388 3 0.0015948636277679426 4 0.0033036460860907386 5 0.008771749952723685 6 0.011733639547149864 7 0.021758496635976933 8 0.03725145759143695 9 0.050352123105245054 10 0.06071873668573668 11 0.056275902294097416 12 0.06595900289125993 13 0.06345278861905315 14 0.06823737950235698 15 0.060035223702407554 16 0.06835129833291184 17 0.06516157107737594 18 0.07757872360785494 19 0.0745029151828739 20 0.07928750606617774 21 0.07860399308284861 22 0.07894574957451317 23 0.08486952876336552 24 0.08088236969394567 25 0.07894574957451317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 877818.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.67310284497324 #Duplication Level Percentage of deduplicated Percentage of total 1 79.74742649180334 45.99281529683718 2 12.226556167591813 14.102868625867293 3 3.4553862183021673 5.978485342117321 4 1.4027688494987944 3.236081284994751 5 0.7536051067852783 2.17313724140622 6 0.46955522846376296 1.6248424189551307 7 0.33121098996913473 1.3371375841512716 8 0.24234734558459006 1.118153870888507 9 0.17241919408316328 0.894955492152511 >10 1.0484526393347904 11.056388836774994 >50 0.08139079708270845 3.3076743372765214 >100 0.06451387822652913 7.112634282706508 >500 0.003771580554789403 1.4067739155906807 >1k 5.955127191772741E-4 0.6580514702811876 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2611 0.29744206657872135 No Hit TATCAACGCAGAGTACTTTTTTTTT 1807 0.20585132681261945 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1330 0.15151204463795456 No Hit GGTATCAACGCAGAGTACTTTTTTT 946 0.107767213704891 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 895 0.10195735334659348 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCG 45 6.768947E-4 14.774674 5 CGCATCG 65 3.3654578E-6 14.616481 13 TAAGACC 65 3.3754277E-6 14.612315 4 TCCAACG 60 2.559798E-5 14.253506 18 ACCCGGT 70 7.248298E-6 13.572448 12 CTAGCAT 70 7.269673E-6 13.56858 4 CGAGCCG 165 0.0 13.244927 15 CGCCGGT 75 1.4774379E-5 12.666172 7 CGCGGTT 100 1.4392936E-7 12.350222 10 AGGCCCG 185 0.0 12.324547 10 GCATCGC 85 3.929468E-6 12.296443 14 CCCGTTT 70 1.08909255E-4 12.215203 12 ACCTCCG 110 3.788955E-8 12.094575 19 ATCGCCA 110 3.7949576E-8 12.093195 16 ATCCCGT 55 0.0030661991 12.091127 10 ATTATAC 55 0.0030753764 12.086304 3 GCAACTT 95 1.0356798E-6 12.002269 14 TCCCGGA 95 1.0356798E-6 12.002269 17 CAACCCG 80 2.8624987E-5 11.875214 10 GTGTTAT 80 2.8641694E-5 11.8745365 1 >>END_MODULE