FastQCFastQC Report
Thu 2 Feb 2017
SRR4063951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877818
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA500.00258160721.9974652
GTATCAA18650.021.1116451
TAATACC1057.7494406E-820.9499684
AGGCGTA654.9307273E-420.30766512
TAACGGC550.00447972820.00225336
GTCGAAC550.004481205320.00111423
TCTAGAT2100.019.902472
CCGATCG1901.8189894E-1218.52312718
TTAACGG600.00740571218.33539835
TCGAATT600.007413016618.33226642
CGACGGT1951.8189894E-1218.050237
CTAGATA2003.6379788E-1217.5979733
ACTAGAC750.001291485617.5979733
TACGACG2003.6379788E-1217.5979735
CTAGGAC1751.9099389E-1017.5979733
CGTCGTA1408.364441E-817.28569210
TCGCACG2055.456968E-1217.17168822
CGATCGC2055.456968E-1217.16777419
ACGGTAT2201.8189894E-1216.9999779
TAGAACA4150.016.96194