##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063951_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	877818
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278288893597534	32.0	32.0	32.0	32.0	32.0
2	31.359763641210364	32.0	32.0	32.0	32.0	32.0
3	31.451897773798212	32.0	32.0	32.0	32.0	32.0
4	31.54600725890788	32.0	32.0	32.0	32.0	32.0
5	31.48084227026559	32.0	32.0	32.0	32.0	32.0
6	35.05899970153266	36.0	36.0	36.0	36.0	36.0
7	35.085291028436416	36.0	36.0	36.0	36.0	36.0
8	35.0272436883272	36.0	36.0	36.0	36.0	36.0
9	35.13422600128956	36.0	36.0	36.0	36.0	36.0
10	34.98889063564429	36.0	36.0	36.0	36.0	36.0
11	35.14401960315236	36.0	36.0	36.0	36.0	36.0
12	35.04820361396098	36.0	36.0	36.0	36.0	36.0
13	35.09683784110146	36.0	36.0	36.0	36.0	36.0
14	35.03584114246917	36.0	36.0	36.0	36.0	36.0
15	35.00301087469156	36.0	36.0	36.0	36.0	36.0
16	35.02041197605882	36.0	36.0	36.0	36.0	36.0
17	34.98660428471506	36.0	36.0	36.0	36.0	36.0
18	34.9789546352433	36.0	36.0	36.0	36.0	36.0
19	34.993022471628514	36.0	36.0	36.0	36.0	36.0
20	34.97475216958413	36.0	36.0	36.0	36.0	36.0
21	34.97150434372501	36.0	36.0	36.0	36.0	36.0
22	34.955636589817026	36.0	36.0	36.0	36.0	36.0
23	34.90702058968944	36.0	36.0	36.0	32.0	36.0
24	34.88517209717732	36.0	36.0	36.0	32.0	36.0
25	34.8582713045301	36.0	36.0	36.0	32.0	36.0
26	34.79342414942505	36.0	36.0	36.0	32.0	36.0
27	34.79801052154319	36.0	36.0	36.0	32.0	36.0
28	34.77344278654573	36.0	36.0	36.0	32.0	36.0
29	34.744305767254716	36.0	36.0	36.0	32.0	36.0
30	34.726051413846605	36.0	36.0	36.0	32.0	36.0
31	34.71571897591528	36.0	36.0	36.0	32.0	36.0
32	34.678383218389236	36.0	36.0	36.0	32.0	36.0
33	34.650062997113295	36.0	36.0	36.0	32.0	36.0
34	34.63883060042059	36.0	36.0	36.0	32.0	36.0
35	34.60053792471788	36.0	36.0	36.0	32.0	36.0
36	34.57209467110494	36.0	36.0	36.0	32.0	36.0
37	34.569635163553265	36.0	36.0	36.0	32.0	36.0
38	34.52600652982737	36.0	36.0	36.0	32.0	36.0
39	34.522926164649164	36.0	36.0	36.0	32.0	36.0
40	34.499393951821446	36.0	36.0	36.0	32.0	36.0
41	34.478756416478134	36.0	36.0	36.0	32.0	36.0
42	34.40813357666396	36.0	36.0	36.0	32.0	36.0
43	34.39646601003853	36.0	36.0	36.0	32.0	36.0
44	34.33953735284535	36.0	36.0	36.0	32.0	36.0
45	34.30012257666168	36.0	36.0	36.0	32.0	36.0
46	34.27603443994085	36.0	36.0	36.0	32.0	36.0
47	34.23880804449214	36.0	36.0	36.0	32.0	36.0
48	34.18517847663183	36.0	36.0	36.0	32.0	36.0
49	34.17339471279924	36.0	36.0	36.0	32.0	36.0
50	33.55770900118248	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	9.0
21	34.0
22	92.0
23	281.0
24	672.0
25	1610.0
26	3207.0
27	6151.0
28	10035.0
29	15626.0
30	22646.0
31	32118.0
32	46841.0
33	74957.0
34	156396.0
35	507141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55606090297178	18.20674836281109	11.916730088273763	26.32046064594337
2	15.761653379873186	20.883011663063016	37.49063013349069	25.864704823573103
3	18.22874243720502	24.822485780065776	28.979502561467825	27.969269221261378
4	12.141468960536239	16.301101139416144	36.673433445201624	34.88399645484599
5	13.909944886069777	37.36264237005849	33.69263332490334	15.034779418968396
6	33.36602814943923	36.46884594134299	16.98114067314867	13.183985236069104
7	29.504521438384725	30.941721404664747	21.106197412219846	18.447559744730686
8	27.66635870574391	33.522021104626596	19.624591458128513	19.187028731500984
9	27.600391884348554	13.99243572071405	18.498991809161435	39.908180585775966
10	15.394316088542977	27.219071075664353	32.31196692682138	25.074645908971295
11	37.034897894552174	21.243583521869	22.857129837847936	18.864388745730892
12	24.296494261908506	23.94926966637731	29.512837513015228	22.241398558698954
13	29.406129067903674	19.81916504237216	25.55726307419428	25.21744281552989
14	23.325013414018702	19.62023802336033	26.007931074158165	31.046817488462803
15	24.963601440904462	27.60913301936016	22.57690406046148	24.850361479273896
16	25.315926476535488	25.965266029858224	24.19120201864858	24.52760547495771
17	23.606146148746095	26.079779635414173	25.661469689616755	24.652604526222976
18	24.26886727970326	25.486434514750243	26.646472609293976	23.59822559625252
19	25.27870241895245	25.067952582425967	25.970759314573183	23.6825856840484
20	25.5700537244877	24.47665331528847	25.6584553412348	24.294837618989032
21	26.372095354617926	24.45882859544917	25.063509748034328	24.10556630189857
22	25.390172883611147	24.62349740720151	25.677135071586754	24.30919463760059
23	24.309250023353464	24.463952500187965	25.919044353359595	25.30775312309897
24	24.36908544302716	25.119245215097063	25.9190738802	24.592595461675778
25	24.710813095376427	24.588804359961085	26.064301223732805	24.63608132092968
26	24.190909753741273	25.418102112611873	26.204749275441568	24.186238858205282
27	24.728885911637143	25.13537191532605	25.613858803076443	24.521883369960364
28	24.26542962341876	25.14240275600598	26.12258940614633	24.46957821442893
29	24.163963896227287	25.171707091454458	25.857851932609027	24.80647707970923
30	24.22267765943752	25.46261117421157	25.901668852081766	24.413042314269145
31	24.711478211335802	25.122529193961835	25.371802905155228	24.79418968954714
32	24.50873953198533	25.35814252335118	25.34709208207402	24.786025862589472
33	24.384811684021056	24.880721820957415	25.684453963407687	25.050012531613845
34	24.797873758391784	25.17005717748945	25.76040137025472	24.271667693864043
35	25.214115320381225	24.931248419340214	25.778709908202114	24.075926352076447
36	24.366594323017242	25.525345910874904	25.50335799853034	24.604701767577513
37	25.10498098608076	25.28384170029324	25.305601188455395	24.3055761251706
38	24.57450529369075	25.183795256698144	25.69237236867739	24.549327080933715
39	24.86602687664903	25.142403404922376	25.2570096652039	24.734560053224698
40	25.177090049920142	25.41381769465096	25.49800524491856	23.911087010510343
41	24.15583646239656	25.48280230396267	26.069843425321714	24.291517808319053
42	25.264893080141764	25.578629470942932	25.41549566134697	23.740981787568337
43	24.58091394082727	24.934979716405618	25.762158479750013	24.721947863017103
44	24.45190240701565	25.51056095346482	25.242393276897367	24.795143362622166
45	24.613982524692133	25.631628712363725	25.225048700743898	24.529340062200248
46	24.31844668157496	25.380976087107587	25.23504490146512	25.065532329852324
47	24.56023631779868	25.46795913880253	25.604436540856074	24.367368002542715
48	25.2404864867328	25.574843586951072	24.79744908886266	24.387220837453462
49	24.609343405288346	25.80207491994345	24.986643001901307	24.601938672866897
50	24.569979505745568	25.91332718923408	24.86127360570148	24.65541969931887
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	41.0
2	32.0
3	96.0
4	160.0
5	176.0
6	192.0
7	171.5
8	151.0
9	227.5
10	304.0
11	457.0
12	610.0
13	1083.5
14	1557.0
15	2076.0
16	2595.0
17	2802.0
18	3009.0
19	3060.0
20	3111.0
21	3497.5
22	3884.0
23	4441.0
24	4998.0
25	5595.0
26	6192.0
27	7746.0
28	9300.0
29	10997.5
30	12695.0
31	14201.0
32	15707.0
33	18207.0
34	20707.0
35	23951.0
36	27195.0
37	30732.5
38	34270.0
39	37190.0
40	40110.0
41	43491.0
42	46872.0
43	48609.0
44	50346.0
45	54463.5
46	58581.0
47	61310.0
48	64039.0
49	65908.0
50	67777.0
51	66316.5
52	64856.0
53	63762.0
54	62668.0
55	61775.0
56	60882.0
57	58232.0
58	55582.0
59	50335.5
60	45089.0
61	39765.5
62	34442.0
63	29929.0
64	25416.0
65	21392.0
66	17368.0
67	14996.0
68	12624.0
69	11228.0
70	9832.0
71	7644.0
72	5456.0
73	4501.0
74	3546.0
75	2786.5
76	2027.0
77	1743.5
78	1460.0
79	1205.5
80	951.0
81	722.0
82	493.0
83	416.5
84	340.0
85	248.0
86	156.0
87	117.0
88	78.0
89	57.5
90	37.0
91	30.0
92	23.0
93	18.5
94	14.0
95	13.0
96	12.0
97	12.0
98	12.0
99	11.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010708370072156187
2	4.5567532221941223E-4
3	1.1391883055485306E-4
4	0.0
5	0.0
6	3.417564916645592E-4
7	0.0
8	1.1391883055485306E-4
9	9.113506444388245E-4
10	7.974318138839713E-4
11	0.0
12	0.0
13	1.1391883055485306E-4
14	5.695941527742652E-4
15	0.00410107789997471
16	3.417564916645592E-4
17	0.0
18	2.2783766110970612E-4
19	0.0
20	6.835129833291184E-4
21	0.0
22	2.2783766110970612E-4
23	4.5567532221941223E-4
24	5.695941527742652E-4
25	0.0013670259666582368
26	0.0047845908833038285
27	0.005809860358297505
28	0.0029618895944261794
29	0.001708782458322796
30	0.0033036460860907386
31	0.0077464804777300075
32	0.0028479707638713265
33	0.004328915561084416
34	0.0028479707638713265
35	0.002278376611097061
36	0.007176886324955743
37	0.004556753222194122
38	0.00854391229161398
39	0.003417564916645592
40	0.001822701288877649
41	0.0029618895944261794
42	0.0012531071361033836
43	0.001708782458322796
44	0.0010252694749936775
45	9.113506444388245E-4
46	7.974318138839713E-4
47	0.0019366201194325019
48	0.0015948636277679426
49	1.1391883055485306E-4
50	0.0012531071361033836
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	877818.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.082645639819965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6909362923259	49.28815867889431
2	11.937590888939106	14.583592681244214
3	3.221082374638425	5.902566998001262
4	1.3287560843974324	3.2465574818001235
5	0.6727631374317742	2.0547076161639275
6	0.44233813724957033	1.6211510214356133
7	0.2794626576193237	1.1949222939446438
8	0.20634241207781473	1.0083152349931892
9	0.17017600618082787	0.935532061374899
>10	0.9241069869541254	10.243033919775417
>50	0.07373080924444497	3.140027957341604
>100	0.05008788207490518	5.807092062394037
>500	0.0026263308663884684	0.9743419926367307
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.1391883055485306E-4
11	0.0	0.0	0.0	0.0	1.1391883055485306E-4
12	0.0	0.0	0.0	0.0	2.2783766110970612E-4
13	0.0	0.0	0.0	0.0	2.2783766110970612E-4
14	0.0	0.0	0.0	0.0	2.2783766110970612E-4
15	0.0	0.0	0.0	0.0	3.417564916645592E-4
16	0.0	0.0	0.0	1.1391883055485306E-4	3.417564916645592E-4
17	0.0	0.0	0.0	1.1391883055485306E-4	3.417564916645592E-4
18	0.0	0.0	0.0	1.1391883055485306E-4	3.417564916645592E-4
19	0.0	0.0	0.0	1.1391883055485306E-4	3.417564916645592E-4
20	0.0	0.0	0.0	1.1391883055485306E-4	3.417564916645592E-4
21	0.0	0.0	0.0	1.1391883055485306E-4	3.417564916645592E-4
22	0.0	0.0	0.0	2.2783766110970612E-4	3.417564916645592E-4
23	0.0	0.0	0.0	6.835129833291184E-4	3.417564916645592E-4
24	0.0	0.0	0.0	0.0015948636277679428	3.417564916645592E-4
25	0.0	0.0	0.0	0.001822701288877649	3.417564916645592E-4
26	0.0	0.0	0.0	0.0019366201194325019	4.5567532221941223E-4
27	0.0	0.0	0.0	0.0027340519333164735	4.5567532221941223E-4
28	0.0	0.0	0.0	0.004328915561084416	4.5567532221941223E-4
29	0.0	0.0	0.0	0.00706296749440089	4.5567532221941223E-4
30	0.0	0.0	0.0	0.013898097327692073	4.5567532221941223E-4
31	0.0	0.0	0.0	0.02756835699427444	4.5567532221941223E-4
32	0.0	0.0	0.0	0.043516993271953866	4.5567532221941223E-4
33	0.0	0.0	0.0	0.05741509059964594	4.5567532221941223E-4
34	0.0	0.0	0.0	0.07461683401342875	4.5567532221941223E-4
35	0.0	0.0	0.0	0.09956505790494156	4.5567532221941223E-4
36	0.0	0.0	0.0	0.12519679477978352	4.5567532221941223E-4
37	0.0	0.0	0.0	0.16837203156007283	4.5567532221941223E-4
38	0.0	0.0	0.0	0.2269263104652673	4.5567532221941223E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTATA	50	0.002581607	21.997465	2
GTATCAA	1865	0.0	21.111645	1
TAATACC	105	7.7494406E-8	20.949968	4
AGGCGTA	65	4.9307273E-4	20.307665	12
TAACGGC	55	0.004479728	20.002253	36
GTCGAAC	55	0.0044812053	20.001114	23
TCTAGAT	210	0.0	19.90247	2
CCGATCG	190	1.8189894E-12	18.523127	18
TTAACGG	60	0.007405712	18.335398	35
TCGAATT	60	0.0074130166	18.332266	42
CGACGGT	195	1.8189894E-12	18.05023	7
CTAGATA	200	3.6379788E-12	17.597973	3
ACTAGAC	75	0.0012914856	17.597973	3
TACGACG	200	3.6379788E-12	17.597973	5
CTAGGAC	175	1.9099389E-10	17.597973	3
CGTCGTA	140	8.364441E-8	17.285692	10
TCGCACG	205	5.456968E-12	17.171688	22
CGATCGC	205	5.456968E-12	17.167774	19
ACGGTAT	220	1.8189894E-12	16.999977	9
TAGAACA	415	0.0	16.9619	4
>>END_MODULE