FastQCFastQC Report
Thu 2 Feb 2017
SRR4063950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884921
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28870.15316291770318227No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG26180.13889176257254282No Hit
TATCAACGCAGAGTACTTTTTTTTT20160.10695408454784046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA453.5385114E-516.8894567
CGAACTT400.00527847914.25047814
CGCCGGT2450.013.5718847
CGGTCCA2650.013.26494910
GTATCAA17700.013.2003281
GCCGGTC2950.012.2380248
CCGGTCC3050.012.1479489
GGTATCA6100.012.1447241
CGTCGTA1750.011.94357410
CCGACCA2250.011.82261859
CGTACAC658.042226E-411.6892863
ACCGAGT907.4578074E-611.611818
TAGGACA3450.011.5643774
CCGTCGT1604.5474735E-1111.2816299
CGACCAT1950.011.20580110
CGCGTAA1451.2169039E-911.138610
TCGCGTA1451.2169039E-911.1383049
AGGACGT7300.011.060845
GGTCCAA3350.011.06007211
ACGAACG1303.2627213E-810.96248815
Produced by FastQC (version 0.11.5)