##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063950_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1884921
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281919507501907	32.0	32.0	32.0	32.0	32.0
2	30.870518711394272	32.0	32.0	32.0	32.0	32.0
3	30.886395769371767	32.0	32.0	32.0	32.0	32.0
4	30.91519114063666	32.0	32.0	32.0	32.0	32.0
5	30.820444464250755	32.0	32.0	32.0	32.0	32.0
6	34.496777318518916	36.0	36.0	36.0	32.0	36.0
7	34.44276391424362	36.0	36.0	36.0	32.0	36.0
8	34.41013496056333	36.0	36.0	36.0	32.0	36.0
9	34.55998261996126	36.0	36.0	36.0	32.0	36.0
10	34.232387988674326	36.0	36.0	36.0	32.0	36.0
11	34.52477212572835	36.0	36.0	36.0	32.0	36.0
12	34.335843252847205	36.0	36.0	36.0	32.0	36.0
13	34.4186684747	36.0	36.0	36.0	32.0	36.0
14	34.314970229521556	36.0	36.0	36.0	32.0	36.0
15	34.25165882283661	36.0	36.0	36.0	32.0	36.0
16	34.24444472739176	36.0	36.0	36.0	32.0	36.0
17	34.183122263479476	36.0	36.0	36.0	32.0	36.0
18	34.178205877063284	36.0	36.0	36.0	32.0	36.0
19	34.19236509116297	36.0	36.0	36.0	32.0	36.0
20	34.17458609671174	36.0	36.0	36.0	32.0	36.0
21	34.160467202604245	36.0	36.0	36.0	32.0	36.0
22	34.13677177982525	36.0	36.0	36.0	32.0	36.0
23	34.07590026319406	36.0	36.0	36.0	32.0	36.0
24	34.062401554229595	36.0	36.0	36.0	32.0	36.0
25	33.66569580369681	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	8.0
4	15.0
5	22.0
6	87.0
7	26.0
8	114.0
9	135.0
10	166.0
11	52.0
12	123.0
13	95.0
14	164.0
15	282.0
16	479.0
17	676.0
18	864.0
19	1159.0
20	1686.0
21	2504.0
22	3985.0
23	6254.0
24	9464.0
25	14251.0
26	20089.0
27	27314.0
28	36876.0
29	50489.0
30	66149.0
31	89499.0
32	126213.0
33	177804.0
34	383578.0
35	864297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38916339820717	17.051221492752777	11.337140491569233	26.22247461747082
2	16.56237730923908	19.207510531509637	38.015831750511985	26.21428040873929
3	19.484138836970068	22.86646361293807	28.306736690316182	29.342660859775677
4	12.754783169694159	15.295638139723414	35.78413635548519	36.16544233509724
5	14.637247005665547	36.39706779659219	34.00796720584318	14.957717991899088
6	34.738333377550205	35.26161356219988	16.708407396598837	13.291645663651074
7	30.522669938306525	30.000350229612653	20.507058187996886	18.969921644083932
8	27.793537512266496	34.08083125066009	19.090944312086265	19.03468692498714
9	26.984073062424297	14.378830326044149	18.53822072232266	40.0988758892089
10	15.831896087240175	27.112274002009755	32.01743699341388	25.03839291733619
11	37.08104636225622	21.111282154724872	22.694573906765896	19.11309757625301
12	24.506338656247344	23.308417565616242	29.464293298224753	22.72095047991166
13	29.778910553429682	19.70427972598202	25.09577038889998	25.421039331688323
14	23.157603023137536	19.73655849471705	25.272482674189828	31.833355807955595
15	24.902496938348126	27.984161691316483	21.95667556894702	25.15666580138837
16	25.390389832398082	25.950319330229398	24.344589967244097	24.314700870128423
17	23.906890027541426	26.131067023126725	25.52078135252385	24.441261596807998
18	24.38574638530676	25.471534821686458	26.548828091815757	23.59389070119102
19	25.540739639551152	24.95676896816274	25.221756478018143	24.280734914267967
20	25.54546096515517	24.94240490770184	25.184360045385834	24.327774081757155
21	25.930446786482253	24.66930367145398	24.6716929039794	24.728556638084367
22	25.644094254069728	24.926091896656082	25.149037389429868	24.280776459844326
23	24.553519042509077	24.884739284855247	25.473374681881488	25.088366990754185
24	24.81562932388393	25.02354741392584	25.566385655138436	24.594437607051802
25	24.85223751584876	24.902836742365515	25.524681591226212	24.720244150559513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	58.0
2	203.5
3	349.0
4	349.0
5	349.0
6	1024.0
7	1699.0
8	1699.0
9	1699.0
10	2132.5
11	2566.0
12	2566.0
13	2566.0
14	3708.5
15	4851.0
16	4851.0
17	4851.0
18	8419.5
19	11988.0
20	11988.0
21	11988.0
22	19525.5
23	27063.0
24	27063.0
25	27063.0
26	41019.5
27	54976.0
28	54976.0
29	54976.0
30	71776.0
31	88576.0
32	88576.0
33	88576.0
34	105125.0
35	121674.0
36	121674.0
37	121674.0
38	141345.5
39	161017.0
40	161017.0
41	161017.0
42	182490.0
43	203963.0
44	203963.0
45	203963.0
46	222475.5
47	240988.0
48	240988.0
49	240988.0
50	245914.5
51	250841.0
52	250841.0
53	250841.0
54	236499.0
55	222157.0
56	222157.0
57	222157.0
58	203299.5
59	184442.0
60	184442.0
61	184442.0
62	159844.5
63	135247.0
64	135247.0
65	135247.0
66	110533.5
67	85820.0
68	85820.0
69	85820.0
70	64590.5
71	43361.0
72	43361.0
73	43361.0
74	32731.0
75	22101.0
76	22101.0
77	22101.0
78	16594.0
79	11087.0
80	11087.0
81	11087.0
82	7567.5
83	4048.0
84	4048.0
85	4048.0
86	2990.5
87	1933.0
88	1933.0
89	1933.0
90	1382.0
91	831.0
92	831.0
93	831.0
94	601.5
95	372.0
96	372.0
97	372.0
98	1642.5
99	2913.0
100	2913.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03984251859892272
2	0.005358314751652722
3	0.0018037891243187381
4	0.004403367568189861
5	0.010345261154180998
6	0.014377260373246414
7	0.023502311237447088
8	0.03841009782372842
9	0.05002862188919323
10	0.058888409646876444
11	0.056288831203005325
12	0.06583830303763394
13	0.06626272400806188
14	0.06976419701409237
15	0.06016167255816026
16	0.06849093410280854
17	0.06435282964113616
18	0.07941977409132797
19	0.07618356419181493
20	0.08032166865348733
21	0.07804040593743716
22	0.07963198457654194
23	0.0839292469021248
24	0.07888924787829305
25	0.079207563606114
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1884921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.5107079936282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.22373108184128	36.48071790885214
2	15.247450239388801	15.403190133782886
3	5.392013182287016	8.170632100448803
4	2.443190597585021	4.9362914738958
5	1.2689423879912156	3.204758921028078
6	0.7764669807745225	2.3531938159557617
7	0.4988469117104081	1.7637977483649359
8	0.34930142906757106	1.4114769988311293
9	0.26332398084471914	1.1970612633750621
>10	1.3763812004474785	12.385596061937232
>50	0.0906946755491489	3.170084870857949
>100	0.06184823545308586	6.160518643165029
>500	0.006120643641409898	2.15531129261791
>1k	0.001688453418319972	1.2073687668873074
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2887	0.15316291770318227	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	2618	0.13889176257254282	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2016	0.10695408454784046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1221048521396919E-4
2	0.0	0.0	0.0	0.0	2.1221048521396919E-4
3	0.0	0.0	0.0	0.0	2.1221048521396919E-4
4	0.0	0.0	0.0	0.0	2.1221048521396919E-4
5	0.0	0.0	0.0	0.0	2.1221048521396919E-4
6	0.0	0.0	0.0	0.0	2.1221048521396919E-4
7	0.0	0.0	0.0	0.0	2.1221048521396919E-4
8	0.0	0.0	0.0	0.0	2.652631065174615E-4
9	0.0	0.0	0.0	0.0	2.652631065174615E-4
10	0.0	0.0	0.0	0.0	2.652631065174615E-4
11	0.0	0.0	0.0	1.0610524260698459E-4	2.652631065174615E-4
12	0.0	0.0	0.0	1.591578639104769E-4	8.488419408558767E-4
13	0.0	0.0	0.0	1.591578639104769E-4	0.001061052426069846
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTTA	45	3.5385114E-5	16.889456	7
CGAACTT	40	0.005278479	14.250478	14
CGCCGGT	245	0.0	13.571884	7
CGGTCCA	265	0.0	13.264949	10
GTATCAA	1770	0.0	13.200328	1
GCCGGTC	295	0.0	12.238024	8
CCGGTCC	305	0.0	12.147948	9
GGTATCA	610	0.0	12.144724	1
CGTCGTA	175	0.0	11.943574	10
CCGACCA	225	0.0	11.8226185	9
CGTACAC	65	8.042226E-4	11.689286	3
ACCGAGT	90	7.4578074E-6	11.61181	8
TAGGACA	345	0.0	11.564377	4
CCGTCGT	160	4.5474735E-11	11.281629	9
CGACCAT	195	0.0	11.205801	10
CGCGTAA	145	1.2169039E-9	11.1386	10
TCGCGTA	145	1.2169039E-9	11.138304	9
AGGACGT	730	0.0	11.06084	5
GGTCCAA	335	0.0	11.060072	11
ACGAACG	130	3.2627213E-8	10.962488	15
>>END_MODULE