FastQCFastQC Report
Thu 2 Feb 2017
SRR4063950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884921
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21350.028.3362451
ACGTAAC651.8145807E-523.6914771
GGTATCA10050.022.984271
ATTCGAC500.002579107222.00273327
TAAGCGT550.00448199220.00195330
TCAACGC30900.019.4346684
ATCAACG31450.019.0248493
CAACGCA31500.018.994655
GATTTCG4750.018.98780341
TATCAAC32200.018.7183512
AACGCAG32450.018.4385666
ATTTCGT4900.018.40654442
TAGGACG8500.018.3743654
CGGATTA600.00741282818.33366620
GTCCTAC10700.018.2984241
TTAGGAC5350.018.0913853
TGTAGGA11900.017.1913072
GTAGGAC11250.016.8158513
TCTAGCG3150.016.76354228
GATATAC6200.016.6768381
Produced by FastQC (version 0.11.5)