##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063950_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1884921
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35767440651359	32.0	32.0	32.0	32.0	32.0
2	31.432071688946117	32.0	32.0	32.0	32.0	32.0
3	31.505620659964	32.0	32.0	32.0	32.0	32.0
4	31.58173419469569	32.0	32.0	32.0	32.0	32.0
5	31.543058303239235	32.0	32.0	32.0	32.0	32.0
6	35.1371930176384	36.0	36.0	36.0	36.0	36.0
7	35.15953931225765	36.0	36.0	36.0	36.0	36.0
8	35.10029545004804	36.0	36.0	36.0	36.0	36.0
9	35.200153215970325	36.0	36.0	36.0	36.0	36.0
10	35.07004166222351	36.0	36.0	36.0	36.0	36.0
11	35.205909425381755	36.0	36.0	36.0	36.0	36.0
12	35.11470507252028	36.0	36.0	36.0	36.0	36.0
13	35.15707554852432	36.0	36.0	36.0	36.0	36.0
14	35.10067318471172	36.0	36.0	36.0	36.0	36.0
15	35.07507529493279	36.0	36.0	36.0	36.0	36.0
16	35.08950030266521	36.0	36.0	36.0	36.0	36.0
17	35.057643795151094	36.0	36.0	36.0	36.0	36.0
18	35.05961470003252	36.0	36.0	36.0	36.0	36.0
19	35.05594398916453	36.0	36.0	36.0	36.0	36.0
20	35.04683220145566	36.0	36.0	36.0	36.0	36.0
21	35.04418487565261	36.0	36.0	36.0	36.0	36.0
22	35.02197545679633	36.0	36.0	36.0	36.0	36.0
23	34.97564354155956	36.0	36.0	36.0	36.0	36.0
24	34.94964987922571	36.0	36.0	36.0	36.0	36.0
25	34.92424457046211	36.0	36.0	36.0	36.0	36.0
26	34.87180364588224	36.0	36.0	36.0	32.0	36.0
27	34.85748845707592	36.0	36.0	36.0	32.0	36.0
28	34.843377520861615	36.0	36.0	36.0	32.0	36.0
29	34.81117192709933	36.0	36.0	36.0	32.0	36.0
30	34.797218557170304	36.0	36.0	36.0	32.0	36.0
31	34.78719108121773	36.0	36.0	36.0	32.0	36.0
32	34.75340292776196	36.0	36.0	36.0	32.0	36.0
33	34.73412731886376	36.0	36.0	36.0	32.0	36.0
34	34.73447163037602	36.0	36.0	36.0	32.0	36.0
35	34.70155407043584	36.0	36.0	36.0	32.0	36.0
36	34.66923335248533	36.0	36.0	36.0	32.0	36.0
37	34.6572742305911	36.0	36.0	36.0	32.0	36.0
38	34.61203148566969	36.0	36.0	36.0	32.0	36.0
39	34.5954912699259	36.0	36.0	36.0	32.0	36.0
40	34.588013502953174	36.0	36.0	36.0	32.0	36.0
41	34.57769742074071	36.0	36.0	36.0	32.0	36.0
42	34.532604814737596	36.0	36.0	36.0	32.0	36.0
43	34.52543528349464	36.0	36.0	36.0	32.0	36.0
44	34.46803499987533	36.0	36.0	36.0	32.0	36.0
45	34.44707974498666	36.0	36.0	36.0	32.0	36.0
46	34.4152619658861	36.0	36.0	36.0	32.0	36.0
47	34.40282643145257	36.0	36.0	36.0	32.0	36.0
48	34.35727916448488	36.0	36.0	36.0	32.0	36.0
49	34.34350617346828	36.0	36.0	36.0	32.0	36.0
50	33.77043812446251	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	4.0
19	4.0
20	18.0
21	55.0
22	174.0
23	512.0
24	1450.0
25	3221.0
26	6570.0
27	12020.0
28	19944.0
29	30678.0
30	44639.0
31	63483.0
32	91816.0
33	149180.0
34	313251.0
35	1147898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5639424788551	17.91070671548702	11.866190065117976	26.659160740539907
2	15.742044464628094	19.982980655881384	37.59274958963645	26.682225289854074
3	18.670145518652586	23.474876559289473	28.618721394568343	29.236256527489594
4	12.34629992450612	15.966080276043398	35.796566540454485	35.891053258996
5	14.15847136299081	36.864621912536386	33.86072944171135	15.116177282761454
6	34.87297835711425	35.56696190544401	16.46779828268118	13.092261454760559
7	30.470805376782767	30.111214328042745	20.519608534902563	18.898371760271925
8	27.721813127347577	34.38326295015173	18.95385480550899	18.941069116991702
9	27.03167136101914	14.216062590423096	18.313120825682574	40.4391452228752
10	15.614139053014286	27.474299793040185	32.04566591349069	24.865895240454833
11	37.227661000116186	21.03679676760989	22.63856151000493	19.096980722268995
12	24.50751121004862	23.4111758826388	29.34825742897827	22.733055478334315
13	29.82084025231974	19.82147688749065	25.046288682218247	25.311394177971362
14	23.10016833632462	19.709886430012567	25.233418765919136	31.95652646774367
15	24.87959202946454	28.052324810249345	21.86071829893434	25.20736486135177
16	25.417527009275787	26.003603358890725	24.26776267064493	24.311106961188557
17	23.827417700794886	26.254309862323144	25.44976686025568	24.46850557662629
18	24.428647673050335	25.436439009949524	26.51250932403282	23.62240399296732
19	25.565382085181334	24.993474524117733	25.147114996922948	24.294028393777985
20	25.564536836811136	24.967266306896676	25.07538829481333	24.392808561478862
21	25.97217602223117	24.641669332560888	24.63853922790398	24.74761541730396
22	25.59174925195764	25.062814336947987	25.122286356980666	24.223150054113702
23	24.576915465855876	24.836503869952203	25.474041955385594	25.112538708806326
24	24.884517093910535	25.02908625393984	25.530064814731308	24.55633183741832
25	24.87330812228631	24.979892704500216	25.4472944811575	24.699504692055974
26	24.299844389231716	25.729839969737338	25.829212274203893	24.141103366827053
27	24.723009950578703	25.26868684725026	25.348111087825597	24.66019211434544
28	24.464640516113974	25.222735966427123	25.482490695570746	24.830132821888157
29	24.39704256407318	25.002387424955273	25.428357090864335	25.172212920107214
30	24.32529221111673	25.38781538931683	25.57520400795181	24.711688391614626
31	25.087742103210708	24.904432070501407	25.064131983223554	24.94369384306433
32	24.750923541923207	25.349004145128852	24.99088793190805	24.909184381039896
33	24.384332762988606	25.138885572931173	25.451911981218416	25.024869682861805
34	24.802186270130207	25.259516121673087	25.665807537347717	24.27249007084899
35	24.945805281000812	25.033397528742036	25.82639651540955	24.194400674847603
36	24.225912274567627	25.3972506946482	25.552707576055823	24.824129454728354
37	24.98178899211555	25.083547806747386	25.143340394906964	24.791322806230102
38	24.554601062940804	25.27076233664461	25.293523722510585	24.881112877904
39	25.33343519825726	24.80310093805923	24.9945221076065	24.868941756077014
40	25.57451933279572	24.841422265608422	25.340552183693394	24.243506217902468
41	24.632108150150064	25.001153931244797	26.02340437835999	24.343333540245144
42	25.22588911259838	25.390143165232892	25.44850216326195	23.935465558906778
43	24.527517092572854	24.767213667904056	25.83184208250592	24.873427157017172
44	24.06494791487059	25.445873642828907	25.459826674695407	25.02935176760509
45	24.327442999507664	25.33412984058537	25.4742436717994	24.864183488107567
46	24.486525028887442	24.887766048314447	25.478459888100286	25.14724903469783
47	24.76891089560656	24.92329746365682	25.742182919964623	24.565608720771998
48	24.723244687454574	25.25776204083235	25.132077513770124	24.886915757942955
49	24.6650641170176	25.164344455155224	25.31358211227786	24.857009315549313
50	24.346288459120448	25.677665180639092	25.11492707005339	24.861119290187073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	11.0
2	15.0
3	75.5
4	136.0
5	179.5
6	223.0
7	209.5
8	196.0
9	251.5
10	307.0
11	473.0
12	639.0
13	1176.5
14	1714.0
15	2326.5
16	2939.0
17	3009.5
18	3080.0
19	3298.5
20	3517.0
21	4256.5
22	4996.0
23	6150.0
24	7304.0
25	9600.0
26	11896.0
27	15856.5
28	19817.0
29	23637.0
30	27457.0
31	31898.5
32	36340.0
33	42403.0
34	48466.0
35	56223.0
36	63980.0
37	71643.5
38	79307.0
39	83259.0
40	87211.0
41	92229.5
42	97248.0
43	99853.5
44	102459.0
45	110166.0
46	117873.0
47	125058.5
48	132244.0
49	136819.5
50	141395.0
51	140549.0
52	139703.0
53	138782.0
54	137861.0
55	136210.5
56	134560.0
57	129912.0
58	125264.0
59	115041.0
60	104818.0
61	91885.0
62	78952.0
63	68794.0
64	58636.0
65	49031.5
66	39427.0
67	33485.5
68	27544.0
69	23283.0
70	19022.0
71	14561.5
72	10101.0
73	8534.0
74	6967.0
75	5581.5
76	4196.0
77	3557.0
78	2918.0
79	2405.0
80	1892.0
81	1482.5
82	1073.0
83	857.5
84	642.0
85	459.0
86	276.0
87	202.5
88	129.0
89	98.0
90	67.0
91	52.0
92	37.0
93	34.5
94	32.0
95	25.0
96	18.0
97	14.5
98	11.0
99	10.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00997389280505655
2	3.7136834912444605E-4
3	4.244209704279383E-4
4	0.0
5	0.0
6	3.183157278209538E-4
7	1.0610524260698458E-4
8	5.305262130349229E-5
9	0.0016976838817117533
10	7.427366982488921E-4
11	0.0
12	2.652631065174615E-4
13	5.835788343384153E-4
14	2.1221048521396916E-4
15	0.004191157082975892
16	6.896840769453998E-4
17	0.0
18	3.7136834912444605E-4
19	5.305262130349229E-5
20	6.896840769453998E-4
21	0.0
22	5.305262130349229E-5
23	7.427366982488921E-4
24	5.30526213034923E-4
25	0.0017507365030152457
26	0.0047747359173143065
27	0.005623577858170184
28	0.0035014730060304914
29	0.0023873679586571533
30	0.0036075782486374762
31	0.0072151564972749525
32	0.0030770520356025533
33	0.005093051645135261
34	0.0035545256273339836
35	0.002705683686478107
36	0.008170103680737813
37	0.0038197887338514455
38	0.007957893195523844
39	0.0038197887338514455
40	0.0021751574734431844
41	0.0031831572782095377
42	0.0019098943669257227
43	0.0020159996095327074
44	0.0013793681538907996
45	0.0013263155325873074
46	0.0010079998047663537
47	0.002228210094746676
48	0.002281262716050169
49	4.244209704279383E-4
50	0.0015915786391047688
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1884921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.17611694224275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9629119057118	40.07023364792641
2	14.45825375135899	15.665840920284888
3	5.076844268202506	8.251311263150814
4	2.231539785029786	4.835846414201636
5	1.1996244477292195	3.2495497183475783
6	0.6979207338846484	2.268638117721035
7	0.47129627145122205	1.787310134160907
8	0.31103525840656676	1.3480546026075881
9	0.25855794831845197	1.260689908000213
>10	1.2073470130442396	11.492180963200564
>50	0.07117232506324875	2.660053078054027
>100	0.04886676948473429	5.136686953071943
>500	0.0037430180416177126	1.4232107432456005
>1k	8.865042730147214E-4	0.5503935360268138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.591578639104769E-4
2	0.0	0.0	0.0	0.0	1.591578639104769E-4
3	0.0	0.0	0.0	0.0	1.591578639104769E-4
4	0.0	0.0	0.0	0.0	1.591578639104769E-4
5	0.0	0.0	0.0	0.0	1.591578639104769E-4
6	0.0	0.0	0.0	0.0	3.7136834912444605E-4
7	0.0	0.0	0.0	0.0	3.7136834912444605E-4
8	0.0	0.0	0.0	0.0	3.7136834912444605E-4
9	0.0	0.0	0.0	0.0	4.2442097042793837E-4
10	0.0	0.0	0.0	0.0	4.2442097042793837E-4
11	0.0	0.0	0.0	1.0610524260698459E-4	4.2442097042793837E-4
12	0.0	0.0	0.0	1.591578639104769E-4	9.018945621593691E-4
13	0.0	0.0	0.0	1.591578639104769E-4	0.0010079998047663537
14	0.0	0.0	0.0	1.591578639104769E-4	0.001061052426069846
15	0.0	0.0	0.0	2.652631065174615E-4	0.0012732629112838152
16	0.0	0.0	0.0	3.7136834912444605E-4	0.0013793681538907996
17	0.0	0.0	0.0	3.7136834912444605E-4	0.001432420775194292
18	0.0	0.0	0.0	4.2442097042793837E-4	0.001432420775194292
19	0.0	0.0	0.0	4.2442097042793837E-4	0.001432420775194292
20	0.0	0.0	0.0	4.2442097042793837E-4	0.0015385260178012766
21	0.0	0.0	0.0	5.30526213034923E-4	0.0015915786391047688
22	0.0	0.0	0.0	5.30526213034923E-4	0.0015915786391047688
23	0.0	0.0	0.0	5.835788343384153E-4	0.001644631260408261
24	0.0	0.0	0.0	9.018945621593691E-4	0.0016976838817117535
25	0.0	0.0	0.0	9.549471834628613E-4	0.0016976838817117535
26	0.0	0.0	0.0	0.0013263155325873074	0.0019098943669257225
27	0.0	0.0	0.0	0.0018568417456222303	0.0019629469882292147
28	0.0	0.0	0.0	0.004350314946886368	0.0019629469882292147
29	0.0	0.0	0.0	0.011512418822857829	0.0019629469882292147
30	0.0	0.0	0.0	0.02238820619007375	0.0019629469882292147
31	0.0	0.0	0.0	0.046580201504466234	0.0020690522308361996
32	0.0	0.0	0.0	0.07331872264142635	0.0020690522308361996
33	0.0	0.0	0.0	0.10148966455358076	0.0020690522308361996
34	0.0	0.0	0.0	0.13167660607526788	0.002334315337353661
35	0.0	0.0	0.0	0.16568333633080642	0.002334315337353661
36	0.0	0.0	0.0	0.21693216850997998	0.002334315337353661
37	0.0	0.0	0.0	0.2920016276544216	0.002334315337353661
38	0.0	0.0	0.0	0.39412792366364424	0.0025465258225676303
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2135	0.0	28.336245	1
ACGTAAC	65	1.8145807E-5	23.691477	1
GGTATCA	1005	0.0	22.98427	1
ATTCGAC	50	0.0025791072	22.002733	27
TAAGCGT	55	0.004481992	20.001953	30
TCAACGC	3090	0.0	19.434668	4
ATCAACG	3145	0.0	19.024849	3
CAACGCA	3150	0.0	18.99465	5
GATTTCG	475	0.0	18.987803	41
TATCAAC	3220	0.0	18.718351	2
AACGCAG	3245	0.0	18.438566	6
ATTTCGT	490	0.0	18.406544	42
TAGGACG	850	0.0	18.374365	4
CGGATTA	60	0.007412828	18.333666	20
GTCCTAC	1070	0.0	18.298424	1
TTAGGAC	535	0.0	18.091385	3
TGTAGGA	1190	0.0	17.191307	2
GTAGGAC	1125	0.0	16.815851	3
TCTAGCG	315	0.0	16.763542	28
GATATAC	620	0.0	16.676838	1
>>END_MODULE