FastQCFastQC Report
Thu 2 Feb 2017
SRR4063949_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063949_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859774
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20060.23331712752420986No Hit
TATCAACGCAGAGTACTTTTTTTTT14970.1741155233817259No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12450.14480549539762777No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9340.10863319895693518No Hit
GCGCAAGACGGACCAGAGCGAAAGC9110.10595807735521195No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9100.10584176772035442No Hit
GAACTACGACGGTATCTGATCGTCT8860.10305033648377365No Hit
GAATAGGACCGCGGTTCTATTTTGT8770.10200354977005585No Hit
GTATCTGATCGTCTTCGAACCTCCG8630.1003752148820504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTATA250.00605033718.988892
TCTAGTA350.0021776316.2761922
CGCCAGT1500.015.83697218
CGTTATT551.13340375E-515.5363652
CGCATCG1500.015.20172313
ATCGCCA1650.014.97313716
GCATCGC1650.014.39640914
TCCAACG400.00526852414.25327418
TCGCCAG1700.013.97461217
CGGTCCA903.6314304E-813.7221810
TTGCGCG707.236311E-613.57454718
CGTCGTA1301.4551915E-1113.15380610
GTCTTAA655.4649845E-513.1492151
CCGCATC1750.013.03004712
CATCGCC1900.013.00223115
TCTTATA957.428207E-812.9923982
CCGTCGT1251.0186341E-1012.919969
TTTGCGC751.4723946E-512.67031517
CGAACGA604.0807985E-412.66957816
GTTATTC751.4812333E-512.6629443
Produced by FastQC (version 0.11.5)