##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063949_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	859774
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.102851447008167	32.0	32.0	32.0	32.0	32.0
2	30.669908603888928	32.0	32.0	32.0	32.0	32.0
3	30.687467869463372	32.0	32.0	32.0	32.0	32.0
4	30.69580261789726	32.0	32.0	32.0	32.0	32.0
5	30.628352334450682	32.0	32.0	32.0	32.0	32.0
6	34.041534170607626	36.0	36.0	36.0	32.0	36.0
7	34.00499549881713	36.0	36.0	36.0	32.0	36.0
8	33.956024490156715	36.0	36.0	36.0	32.0	36.0
9	34.17316411056859	36.0	36.0	36.0	32.0	36.0
10	33.782499819720066	36.0	36.0	36.0	32.0	36.0
11	34.161876260505665	36.0	36.0	36.0	32.0	36.0
12	33.92156659773382	36.0	36.0	36.0	32.0	36.0
13	34.07270050036405	36.0	36.0	36.0	32.0	36.0
14	33.918683281885706	36.0	36.0	36.0	32.0	36.0
15	33.82630900678551	36.0	36.0	36.0	32.0	36.0
16	33.837425881685185	36.0	36.0	36.0	32.0	36.0
17	33.75393301030271	36.0	36.0	36.0	32.0	36.0
18	33.765024297082725	36.0	36.0	36.0	32.0	36.0
19	33.74264864952883	36.0	36.0	36.0	32.0	36.0
20	33.72204207152112	36.0	36.0	36.0	32.0	36.0
21	33.69191089751493	36.0	36.0	36.0	27.0	36.0
22	33.66229381209481	36.0	36.0	36.0	27.0	36.0
23	33.611831713915514	36.0	36.0	36.0	27.0	36.0
24	33.58135858958284	36.0	36.0	36.0	27.0	36.0
25	32.86289536552629	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	8.0
5	6.0
6	30.0
7	17.0
8	44.0
9	68.0
10	70.0
11	25.0
12	59.0
13	45.0
14	134.0
15	316.0
16	512.0
17	666.0
18	866.0
19	1055.0
20	1364.0
21	1681.0
22	2359.0
23	3212.0
24	4436.0
25	6135.0
26	9124.0
27	12796.0
28	18197.0
29	25811.0
30	36805.0
31	54925.0
32	84064.0
33	123095.0
34	230736.0
35	241110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12076642671231	17.760966364115518	11.468011062644495	25.650256146527685
2	16.48364430269468	19.95554423418488	38.24498593164606	25.315825531474378
3	18.738114429475168	24.153689222935213	29.232309909381794	27.875886438207825
4	12.206512155915817	15.433860698392316	36.97696761601683	35.382659529675045
5	14.019178604828616	37.08933730987731	34.403309030508034	14.488175054786042
6	33.53627895290297	36.2380070538531	17.085469807089215	13.140244186154721
7	29.31485973208625	30.9609646291029	21.435310404197278	18.28886523461357
8	27.455714244160678	33.61656825387591	19.846185171180082	19.081532330783325
9	26.941663689710825	14.260180898067093	19.104958347830237	39.693197064391846
10	15.061689316727685	27.16901778877373	32.69301040607188	25.076282488426706
11	36.72898447000774	21.23890818743054	23.163370398170617	18.868736944391106
12	24.25228135797972	23.873810441002412	29.84729652823926	22.026611672778607
13	28.997676973641333	19.890505851791847	25.938521270355576	25.17329590421124
14	22.73212914038314	20.275947952794397	26.10891273480599	30.88301017201648
15	24.512931280279957	28.004022004648117	22.87224199992319	24.61080471514874
16	24.80052046236424	26.255999515848966	24.78527436001285	24.15820566177395
17	23.059716517911696	26.43408209319133	26.302570983994052	24.20363040490292
18	23.972692829248704	25.565960319631248	27.163068972139936	23.298277878980116
19	24.86998687065265	25.44858603447152	26.055236374810274	23.626190720065555
20	24.929433031510406	24.95294565441333	25.912772824995084	24.20484848908118
21	25.858663454207914	24.80378977018117	25.317084810075013	24.020461965535905
22	24.90013129591865	25.08077958115671	25.886713039267722	24.13237608365692
23	23.672971466020083	24.881472177827234	26.352113479404153	25.093442876748522
24	24.055534798291774	25.62757744108332	26.17754793472985	24.139339825895057
25	23.981138861697012	25.440886673704867	26.163834896365007	24.41413956823311
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	68.0
2	221.5
3	375.0
4	375.0
5	375.0
6	1067.5
7	1760.0
8	1760.0
9	1760.0
10	1928.0
11	2096.0
12	2096.0
13	2096.0
14	2496.5
15	2897.0
16	2897.0
17	2897.0
18	4778.5
19	6660.0
20	6660.0
21	6660.0
22	10896.0
23	15132.0
24	15132.0
25	15132.0
26	22085.0
27	29038.0
28	29038.0
29	29038.0
30	36387.5
31	43737.0
32	43737.0
33	43737.0
34	52644.0
35	61551.0
36	61551.0
37	61551.0
38	69148.5
39	76746.0
40	76746.0
41	76746.0
42	86208.5
43	95671.0
44	95671.0
45	95671.0
46	104762.0
47	113853.0
48	113853.0
49	113853.0
50	113759.5
51	113666.0
52	113666.0
53	113666.0
54	103850.0
55	94034.0
56	94034.0
57	94034.0
58	84479.0
59	74924.0
60	74924.0
61	74924.0
62	65411.0
63	55898.0
64	55898.0
65	55898.0
66	45459.0
67	35020.0
68	35020.0
69	35020.0
70	26147.5
71	17275.0
72	17275.0
73	17275.0
74	13217.5
75	9160.0
76	9160.0
77	9160.0
78	7548.0
79	5936.0
80	5936.0
81	5936.0
82	4041.5
83	2147.0
84	2147.0
85	2147.0
86	1630.5
87	1114.0
88	1114.0
89	1114.0
90	773.0
91	432.0
92	432.0
93	432.0
94	281.0
95	130.0
96	130.0
97	130.0
98	292.0
99	454.0
100	454.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03547443863154736
2	0.00500131429887389
3	0.0015120252531479201
4	0.003256669776010905
5	0.007676435900597134
6	0.011863582755468298
7	0.019656328290922963
8	0.03477658082240217
9	0.04594230576872527
10	0.05443290911332513
11	0.05454921874818266
12	0.061295177569919534
13	0.06397029917164279
14	0.06443553771107291
15	0.05827112706362369
16	0.0625745835533524
17	0.06176041610934966
18	0.0766480493711138
19	0.07327506996024537
20	0.07688066864082888
21	0.07199566397681251
22	0.07513602411796588
23	0.0806025769562699
24	0.0740892374042481
25	0.07595019156196861
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	859774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.67138322616735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4989775962407	48.03485341459939
2	12.35837121591073	14.7488220975169
3	3.421376575111105	6.124748183234599
4	1.3131655127430648	3.134336102011119
5	0.6292424409948465	1.8773883419386244
6	0.38060124554185765	1.3626601667450884
7	0.23799209980281721	0.9940922454493921
8	0.16793398287340797	0.8016682438988602
9	0.13771459703259475	0.7395858445832254
>10	0.7032485281162697	7.8416577967252685
>50	0.07373387859047875	3.1308343785941504
>100	0.07138400347233734	8.308169245050632
>500	0.00567160573518543	2.3470881494085343
>1k	5.867178346743548E-4	0.5540957902442184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2006	0.23331712752420986	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1497	0.1741155233817259	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1245	0.14480549539762777	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	934	0.10863319895693518	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	911	0.10595807735521195	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	910	0.10584176772035442	No Hit
GAACTACGACGGTATCTGATCGTCT	886	0.10305033648377365	No Hit
GAATAGGACCGCGGTTCTATTTTGT	877	0.10200354977005585	No Hit
GTATCTGATCGTCTTCGAACCTCCG	863	0.1003752148820504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1630963485753232E-4
2	0.0	0.0	0.0	0.0	1.1630963485753232E-4
3	0.0	0.0	0.0	0.0	1.1630963485753232E-4
4	0.0	0.0	0.0	0.0	1.1630963485753232E-4
5	0.0	0.0	0.0	0.0	1.1630963485753232E-4
6	0.0	0.0	0.0	0.0	2.3261926971506464E-4
7	0.0	0.0	0.0	0.0	2.3261926971506464E-4
8	0.0	0.0	0.0	0.0	2.3261926971506464E-4
9	0.0	0.0	0.0	1.1630963485753232E-4	2.3261926971506464E-4
10	0.0	0.0	0.0	3.48928904572597E-4	4.652385394301293E-4
11	0.0	0.0	0.0	4.652385394301293E-4	4.652385394301293E-4
12	0.0	0.0	0.0	4.652385394301293E-4	0.0011630963485753233
13	0.0	0.0	0.0	4.652385394301293E-4	0.0011630963485753233
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTATA	25	0.006050337	18.98889	2
TCTAGTA	35	0.00217763	16.276192	2
CGCCAGT	150	0.0	15.836972	18
CGTTATT	55	1.13340375E-5	15.536365	2
CGCATCG	150	0.0	15.201723	13
ATCGCCA	165	0.0	14.973137	16
GCATCGC	165	0.0	14.396409	14
TCCAACG	40	0.005268524	14.253274	18
TCGCCAG	170	0.0	13.974612	17
CGGTCCA	90	3.6314304E-8	13.72218	10
TTGCGCG	70	7.236311E-6	13.574547	18
CGTCGTA	130	1.4551915E-11	13.153806	10
GTCTTAA	65	5.4649845E-5	13.149215	1
CCGCATC	175	0.0	13.030047	12
CATCGCC	190	0.0	13.002231	15
TCTTATA	95	7.428207E-8	12.992398	2
CCGTCGT	125	1.0186341E-10	12.91996	9
TTTGCGC	75	1.4723946E-5	12.670315	17
CGAACGA	60	4.0807985E-4	12.669578	16
GTTATTC	75	1.4812333E-5	12.662944	3
>>END_MODULE