FastQCFastQC Report
Thu 2 Feb 2017
SRR4063949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859774
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9010.10479498100663663No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8810.10246878830948598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1451.2732926E-1121.24042310
GTATCAA15500.021.1474361
TCCCCGC1850.020.215311
GTCGTAG1653.6379788E-1219.99910211
ATACCGT1653.6379788E-1219.9991026
TACCGTC1653.6379788E-1219.9991027
GCCGGTC3150.018.8562978
CGGTCCA2800.018.85629510
CCGTCGT1658.185452E-1118.6658299
ACGAAAA2600.018.61563123
GTCCTAA1303.585592E-818.6145481
GTATTAC1801.4551915E-1118.332511
GGGTATA600.00741232618.332511
CGGTGCG600.00741232618.3325114
TACACGG600.00741232618.332515
CGCCGGT2900.018.2060787
ACCGTCG1701.2732926E-1018.1168338
AACGCAG18300.017.6713376
CCTTATA1002.4826197E-517.5992092
TATATGG750.001290847917.5992092
Produced by FastQC (version 0.11.5)