##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	859774
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.042571652550553	32.0	32.0	32.0	32.0	32.0
2	30.802612081779632	32.0	32.0	32.0	32.0	32.0
3	31.140624164024498	32.0	32.0	32.0	32.0	32.0
4	31.33824935389998	32.0	32.0	32.0	32.0	32.0
5	31.180313663823284	32.0	32.0	32.0	32.0	32.0
6	34.78590769202139	36.0	36.0	36.0	32.0	36.0
7	34.81704610746545	36.0	36.0	36.0	32.0	36.0
8	34.72373670290099	36.0	36.0	36.0	32.0	36.0
9	34.94665691216529	36.0	36.0	36.0	32.0	36.0
10	34.66757659571004	36.0	36.0	36.0	32.0	36.0
11	34.95646065128743	36.0	36.0	36.0	36.0	36.0
12	34.79793876065106	36.0	36.0	36.0	32.0	36.0
13	34.883674081793586	36.0	36.0	36.0	32.0	36.0
14	34.818753532905156	36.0	36.0	36.0	32.0	36.0
15	34.73811955234748	36.0	36.0	36.0	32.0	36.0
16	34.77815216556909	36.0	36.0	36.0	32.0	36.0
17	34.73475471461105	36.0	36.0	36.0	32.0	36.0
18	34.74331045135117	36.0	36.0	36.0	32.0	36.0
19	34.74205430729471	36.0	36.0	36.0	32.0	36.0
20	34.729639416870015	36.0	36.0	36.0	32.0	36.0
21	34.73314266307192	36.0	36.0	36.0	32.0	36.0
22	34.71079725602309	36.0	36.0	36.0	32.0	36.0
23	34.66280324829548	36.0	36.0	36.0	32.0	36.0
24	34.62293230546632	36.0	36.0	36.0	32.0	36.0
25	34.583230011607704	36.0	36.0	36.0	32.0	36.0
26	34.52396560026239	36.0	36.0	36.0	32.0	36.0
27	34.51921784096751	36.0	36.0	36.0	32.0	36.0
28	34.492924884911616	36.0	36.0	36.0	32.0	36.0
29	34.45789707527792	36.0	36.0	36.0	32.0	36.0
30	34.434692140027494	36.0	36.0	36.0	32.0	36.0
31	34.42705524940275	36.0	36.0	36.0	32.0	36.0
32	34.37958928741739	36.0	36.0	36.0	32.0	36.0
33	34.358811734246444	36.0	36.0	36.0	32.0	36.0
34	34.33294679764683	36.0	36.0	36.0	32.0	36.0
35	34.306617785604125	36.0	36.0	36.0	32.0	36.0
36	34.258406278859326	36.0	36.0	36.0	32.0	36.0
37	34.256488332980524	36.0	36.0	36.0	32.0	36.0
38	34.2084291918574	36.0	36.0	36.0	32.0	36.0
39	34.202891690141826	36.0	36.0	36.0	32.0	36.0
40	34.19076641070793	36.0	36.0	36.0	32.0	36.0
41	34.169318914040204	36.0	36.0	36.0	32.0	36.0
42	34.08696704017567	36.0	36.0	36.0	32.0	36.0
43	34.07225619755889	36.0	36.0	36.0	32.0	36.0
44	34.00971766999235	36.0	36.0	36.0	32.0	36.0
45	33.973587245020205	36.0	36.0	36.0	32.0	36.0
46	33.950816144707794	36.0	36.0	36.0	32.0	36.0
47	33.92487676994187	36.0	36.0	36.0	32.0	36.0
48	33.870254276123724	36.0	36.0	36.0	32.0	36.0
49	33.86243129008321	36.0	36.0	36.0	32.0	36.0
50	33.13196723790205	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	3.0
20	3.0
21	18.0
22	70.0
23	229.0
24	593.0
25	1363.0
26	3108.0
27	5912.0
28	10567.0
29	17160.0
30	26736.0
31	41169.0
32	62340.0
33	106109.0
34	203724.0
35	380667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44383986116556	19.151732697163872	12.34074923609649	26.06367820557408
2	15.623051800020471	20.95297801267319	37.452196406472446	25.9717737808339
3	18.092354717297145	24.886597842218634	29.372554584238614	27.64849285624561
4	12.088641898917622	16.415825554157255	37.022985110040544	34.472547436884575
5	14.054507347279635	37.18395764468337	33.708625755140304	15.05290925289669
6	33.7425111046753	36.99964990759189	16.43259325426595	12.825245733466856
7	29.397260210241292	31.1344609164734	21.19266225775611	18.2756166155292
8	27.51176471956584	34.053134893588314	19.356016813720814	19.07908357312503
9	26.91391970150052	13.947150797832435	18.807770012107934	40.33115948855911
10	14.999494050146028	27.37863309796003	32.49989241296208	25.12198043893185
11	37.142784033943805	21.253492196786596	22.815065354383826	18.788658414885774
12	24.08833494422365	23.93050258614774	29.705050053909577	22.276112415719034
13	29.182853128503837	19.87957272393146	25.71565484802948	25.221919299535227
14	22.92782272509456	20.03891729435246	25.759154752655355	31.274105227897632
15	24.590665415138606	28.045743583301636	22.671849574701454	24.691741426858304
16	25.064930080219032	26.295606620832757	24.404637979182827	24.23482531976538
17	23.22342848236862	26.745400535489562	25.783752474487482	24.247418507654338
18	24.26436807010239	25.531333343413536	26.84517156312553	23.359127023358546
19	25.240586479702802	25.45610823309381	25.722340987282706	23.580964299920677
20	25.314474004063882	25.00567012767383	25.538138732613064	24.141717135649227
21	25.995901248467625	24.81791726663053	25.11683302821439	24.069348456687457
22	25.01378269173062	25.159053425667675	25.555669280531863	24.271494602069847
23	23.748288772603612	24.957342895658456	26.083932432542454	25.210435899195478
24	24.321504588436444	25.6162694674157	25.812484734289402	24.24974120985845
25	24.38912900869549	25.369988694466798	25.815114986112338	24.42576731072537
26	24.01091960574035	25.95779179238326	26.169833135210958	23.861455466665426
27	24.482071550953037	25.583007550106256	25.540784648078507	24.394136250862196
28	23.724941697829006	25.510238500950866	26.21602917144037	24.54879062977976
29	23.97112652384237	25.393864037378265	26.01706773240159	24.617941706377778
30	23.89508064009603	25.681073247214876	26.27310856927511	24.150737543413985
31	24.51573730180794	25.41336661591306	25.397082035898194	24.67381404638081
32	24.258123048113102	25.64097494024391	25.236320071649153	24.864581939993833
33	23.843221822487575	25.45243487252497	25.89396524751174	24.810378057475717
34	24.49529108941754	25.41439227312961	26.146230370815804	23.944086266637047
35	25.061994621704862	25.379876987782524	25.870134643478586	23.687993747034028
36	23.97833253074831	26.031321752845688	25.515689955590293	24.474655760815704
37	24.798660060018147	25.78372531230372	25.361853583641565	24.05576104403657
38	24.08523854112748	25.505609482322424	25.863173995731092	24.545977980819004
39	24.645501230030185	25.404688654062006	25.378750428911236	24.571059686996573
40	24.891015083419404	25.58516874633615	25.828262103490307	23.695554066754134
41	23.970312334617812	25.77106627383975	26.243179711335024	24.01544168020741
42	25.1301233714951	26.1541288800027	25.425321688236615	23.290426060265585
43	24.324641821146685	25.450529562694967	25.77596694914663	24.448861667011723
44	24.16433365939218	25.833281962587364	25.369316893143807	24.633067484876648
45	24.294136872603264	26.06299643041164	25.336284534226294	24.306582162758808
46	24.0399970689597	25.659629456024508	25.442941833970522	24.857431641045267
47	24.414164500351845	25.541229768945804	25.773156306156984	24.271449424545366
48	24.98345455031852	26.06492795023687	24.8988953804174	24.05272211902721
49	24.27759581654586	26.211722232043993	25.13212866726838	24.37855328414177
50	24.42481375724762	26.205649218100298	25.02474513384471	24.34479189080737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	13.5
2	18.0
3	38.0
4	58.0
5	80.0
6	102.0
7	109.5
8	117.0
9	164.5
10	212.0
11	317.5
12	423.0
13	737.0
14	1051.0
15	1457.0
16	1863.0
17	2099.0
18	2335.0
19	2491.5
20	2648.0
21	3208.5
22	3769.0
23	4491.0
24	5213.0
25	6074.5
26	6936.0
27	8679.5
28	10423.0
29	12129.0
30	13835.0
31	15653.0
32	17471.0
33	20157.0
34	22843.0
35	26002.0
36	29161.0
37	33251.5
38	37342.0
39	39944.5
40	42547.0
41	45580.5
42	48614.0
43	49259.5
44	49905.0
45	54046.5
46	58188.0
47	61179.5
48	64171.0
49	64929.5
50	65688.0
51	63116.0
52	60544.0
53	59150.5
54	57757.0
55	57402.5
56	57048.0
57	54425.0
58	51802.0
59	46573.5
60	41345.0
61	36247.5
62	31150.0
63	26960.5
64	22771.0
65	19421.0
66	16071.0
67	13677.5
68	11284.0
69	10552.0
70	9820.0
71	7476.5
72	5133.0
73	4443.0
74	3753.0
75	2904.5
76	2056.0
77	1820.0
78	1584.0
79	1297.5
80	1011.0
81	859.5
82	708.0
83	594.5
84	481.0
85	348.0
86	215.0
87	158.5
88	102.0
89	76.0
90	50.0
91	56.0
92	62.0
93	43.5
94	25.0
95	26.5
96	28.0
97	24.5
98	21.0
99	16.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005233933568588955
2	6.97857809145194E-4
3	2.3261926971506464E-4
4	0.0
5	0.0
6	1.1630963485753232E-4
7	0.0
8	0.0
9	8.141674440027263E-4
10	5.815481742876617E-4
11	0.0
12	1.1630963485753232E-4
13	2.3261926971506464E-4
14	2.3261926971506464E-4
15	0.002907740871438308
16	3.48928904572597E-4
17	0.0
18	3.48928904572597E-4
19	0.0
20	5.815481742876617E-4
21	0.0
22	0.0
23	0.0012794059834328555
24	4.652385394301293E-4
25	0.0020935734274355818
26	0.0041871468548711635
27	0.005931791377734149
28	0.0033729794108684377
29	0.002675121601723243
30	0.003256669776010905
31	0.007560126265739602
32	0.0033729794108684377
33	0.004303456489728697
34	0.002442502332008179
35	0.002558811966865711
36	0.0060481010125916805
37	0.003954527585156099
38	0.008025364805169731
39	0.004536075759443761
40	0.002558811966865711
41	0.002675121601723243
42	0.0015120252531479201
43	9.304770788602586E-4
44	0.0012794059834328555
45	0.0012794059834328555
46	0.0012794059834328555
47	0.002209883062293114
48	0.002442502332008179
49	6.97857809145194E-4
50	0.0010467867137177909
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	859774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.05559713526698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.04226277653488	52.55266132479427
2	11.598142851842189	14.85851932069759
3	3.1116717631205653	5.9795997862691035
4	1.1633436637595178	2.9807469222258063
5	0.5707035665288307	1.8278378870615404
6	0.3493063467848522	1.3425015975865409
7	0.21618689129031626	0.9693586290092777
8	0.14924904365372038	0.7648189290485269
9	0.10539128327988416	0.6075811425010552
>10	0.5744206680995722	6.927061312567671
>50	0.06130464803873337	2.774273184416644
>100	0.05436766080626013	6.841697336615329
>500	0.0036488362607584542	1.5733426272066215
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	901	0.10479498100663663	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	881	0.10246878830948598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.1630963485753232E-4	0.0
6	0.0	0.0	0.0	1.1630963485753232E-4	0.0
7	0.0	0.0	0.0	1.1630963485753232E-4	0.0
8	0.0	0.0	0.0	1.1630963485753232E-4	0.0
9	0.0	0.0	0.0	1.1630963485753232E-4	0.0
10	0.0	0.0	0.0	2.3261926971506464E-4	0.0
11	0.0	0.0	0.0	2.3261926971506464E-4	0.0
12	0.0	0.0	0.0	2.3261926971506464E-4	0.0
13	0.0	0.0	0.0	2.3261926971506464E-4	0.0
14	0.0	0.0	0.0	2.3261926971506464E-4	0.0
15	0.0	0.0	0.0	3.48928904572597E-4	0.0
16	0.0	0.0	0.0	3.48928904572597E-4	0.0
17	0.0	0.0	0.0	3.48928904572597E-4	0.0
18	0.0	0.0	0.0	4.652385394301293E-4	0.0
19	0.0	0.0	0.0	4.652385394301293E-4	1.1630963485753232E-4
20	0.0	0.0	0.0	4.652385394301293E-4	1.1630963485753232E-4
21	0.0	0.0	0.0	6.97857809145194E-4	2.3261926971506464E-4
22	0.0	0.0	0.0	8.141674440027263E-4	2.3261926971506464E-4
23	0.0	0.0	0.0	0.0010467867137177909	2.3261926971506464E-4
24	0.0	0.0	0.0	0.0015120252531479203	2.3261926971506464E-4
25	0.0	0.0	0.0	0.0016283348880054525	2.3261926971506464E-4
26	0.0	0.0	0.0	0.0018609541577205172	2.3261926971506464E-4
27	0.0	0.0	0.0	0.0023261926971506466	2.3261926971506464E-4
28	0.0	0.0	0.0	0.0030240505062958407	2.3261926971506464E-4
29	0.0	0.0	0.0	0.005699172108019084	2.3261926971506464E-4
30	0.0	0.0	0.0	0.009421080423460118	2.3261926971506464E-4
31	0.0	1.1630963485753232E-4	0.0	0.015818110340624397	2.3261926971506464E-4
32	0.0	1.1630963485753232E-4	0.0	0.023610855876079063	2.3261926971506464E-4
33	0.0	2.3261926971506464E-4	0.0	0.030938362872103598	2.3261926971506464E-4
34	0.0	2.3261926971506464E-4	0.0	0.0391963469469884	3.48928904572597E-4
35	0.0	2.3261926971506464E-4	0.0	0.05222302605103202	3.48928904572597E-4
36	0.0	2.3261926971506464E-4	0.0	0.06804113639165642	3.48928904572597E-4
37	0.0	2.3261926971506464E-4	0.0	0.09293139825116833	3.48928904572597E-4
38	0.0	3.48928904572597E-4	0.0	0.1285221465175732	3.48928904572597E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	145	1.2732926E-11	21.240423	10
GTATCAA	1550	0.0	21.147436	1
TCCCCGC	185	0.0	20.21531	1
GTCGTAG	165	3.6379788E-12	19.999102	11
ATACCGT	165	3.6379788E-12	19.999102	6
TACCGTC	165	3.6379788E-12	19.999102	7
GCCGGTC	315	0.0	18.856297	8
CGGTCCA	280	0.0	18.856295	10
CCGTCGT	165	8.185452E-11	18.665829	9
ACGAAAA	260	0.0	18.615631	23
GTCCTAA	130	3.585592E-8	18.614548	1
GTATTAC	180	1.4551915E-11	18.33251	1
GGGTATA	60	0.007412326	18.33251	1
CGGTGCG	60	0.007412326	18.33251	14
TACACGG	60	0.007412326	18.33251	5
CGCCGGT	290	0.0	18.206078	7
ACCGTCG	170	1.2732926E-10	18.116833	8
AACGCAG	1830	0.0	17.671337	6
CCTTATA	100	2.4826197E-5	17.599209	2
TATATGG	75	0.0012908479	17.599209	2
>>END_MODULE