FastQCFastQC Report
Thu 2 Feb 2017
SRR4063948_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063948_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519220
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT51330.9885982820384424No Hit
CTTTAGGACGTGAAATATGGCGAGG44040.848195369978044No Hit
GTCCTACAGTGGACATTTCTAAATT43440.8366395747467354No Hit
CTGTAGGACGTGGAATATGGCAAGA42820.8246985863410501No Hit
GTATCAACGCAGAGTACTTTTTTTT39750.7655714340741882No Hit
GTCCTACAGTGTGCATTTCTCATTT32920.6340279650244598No Hit
TATCAACGCAGAGTACTTTTTTTTT28670.5521744154693579No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25520.49150649050498824No Hit
CTGTAGGACCTGGAATATGGCGAGA24120.4645429682986017No Hit
ATTTAGAAATGTCCACTGTAGGACG21040.4052232194445515No Hit
CTGAAGGACCTGGAATATGGCGAGA19600.37748931088941107No Hit
GTACATGGGGTGGTATCAACGCAAA19200.369785447401872No Hit
GTCCTTCAGTGTGCATTTCTCATTT17910.34494048765455876No Hit
GATATACACTGTTCTACAAATCCCG16090.3098879087862563No Hit
TTTCTAAATTTTCCACCTTTTTCAG15400.29659874427025157No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15060.29005046030584336No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15060.29005046030584336No Hit
GAATATGGCAAGAAAACTGAAAATC14630.28176880705673896No Hit
TTGTAGAACAGTGTATATCAATGAG14190.27329455722044604No Hit
GGTATCAACGCAGAGTACTTTTTTT14180.27310196063325753No Hit
GTATCAACGCAGAGTACATGGGGTG12040.23188629097492391No Hit
CTGTAGGACATGGAATATGGCAAGA11810.22745656946958898No Hit
GGAATATGGCGAGAAAACTGAAAAT11610.22360463772581948No Hit
GTGTATATCAATGAGTTACAATGAA11310.21782674011016526No Hit
GTGTATATCAATGAGTTACAATGAG11200.21570817765109201No Hit
GCCATATTCCACGTCCTACAGTGGA10420.20068564385039098No Hit
GAAATATGGCGAGGAAAACTGAAAA10090.19432995647317128No Hit
GTTCTACAGTGTGGTTTTTATCATT9800.18874465544470553No Hit
CCATATTTCACGTCCTAAAGTGTGT9430.18161858171873194No Hit
CCATATTCCAGGTCCTTCAGTGTGC9190.17699626362620854No Hit
ATTCCAGGTCCTTCAGTGTGCATTT9180.1768036670390201No Hit
GATATACACTGTTCTACAATGCCGG8780.16909980355148108No Hit
TTCCAGGTCCTTCAGTGTGCATTTC8780.16909980355148108No Hit
ACAGTGGACATTTCTAAATTTTCCA8760.16871461037710414No Hit
GTACATGGGTGGTATCAACGCAAAA8340.16062555371518816No Hit
ATACACACTTTAGGACGTGAAATAT8300.15985516736643426No Hit
GTGTAAAGGTATAAGGGAGCTTGAC8100.15600323562266477No Hit
CCTAAAGTGTGTATTTCTCATTTTC7980.15369207657640307No Hit
GACCTGGAATATGGCGAGAAAACTG7850.1511883209429529No Hit
GTCCACTGTAGGACGTGGAATATGG7810.150417934594199No Hit
CTGTAGAACATATTAGATGAGTGAG7780.14984014483263355No Hit
TATCAACGCAGAGTACATGGGGTGG7770.1496475482454451No Hit
CCCTTATACCTTTACACTCTGCGAA7730.14887716189669117No Hit
ACCTGGAATATGGCGAGAAAACTGA7610.1465660028504295No Hit
CCTTTACACTCTGCGAATGATTTCC7580.14598821308886406No Hit
GTGTATTTCTCATTTTCCGTGATTT7510.14464003697854472No Hit
CCACTGTAGGACGTGGAATATGGCA7350.14155849158352915No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7280.1402103154732098No Hit
GTTCTACAAATCCCGTTTCCAACGA7210.1388621393628905No Hit
CACTTTAGGACGTGAAATATGGCGA7050.1357805939678749No Hit
GAAATACACACTTTAGGACGTGAAA6830.13154346904972844No Hit
GTAGGACGTGGAATATGGCAAGAAA6720.1294249065906552No Hit
GTATCAACGCAGAGTACATGGGAAA6550.12615076460845115No Hit
CTACAGTGGACATTTCTAAATTTTC6380.12287662262624706No Hit
TCCTAAAGTGTGTATTTCTCATTTT6250.12037286699279688No Hit
ATGTTCTACAGTGTGGTTTTTATCA6150.11844690112091213No Hit
GTAGAACATATTAGATGAGTGAGTT6120.1178691113593467No Hit
GGTATCAACGCAGAGTACATGGGGT6010.11575054890027349No Hit
AGTGTGTATTTCTCATTTTCCGTGA5850.1126690035052579No Hit
ATATTTCACGTCCTAAAGTGTGTAT5800.1117060205693155No Hit
ATTCCACGTCCTACAGTGGACATTT5670.10920226493586535No Hit
GTACATGGGAGTGGTATCAACGCAA5540.10669850930241516No Hit
GTATTTCTCATTTTCCGTGATTTTC5430.10457994684334193No Hit
GTATAAGGGAGCTTGACTGCGAGAG5210.10034282192519549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG359.924169E-518.99980711
GAAAGCG250.00602713218.9998079
TTATACC701.3060344E-917.6409784
CCTAGAG659.995347E-917.5365923
AGGCCCA402.756882E-416.6248325
GTTCCCT402.756882E-416.6248329
CCCTTAT804.292815E-1016.623231
ACTGTGC753.265086E-916.46658
CCTTATA753.268724E-916.4649122
TCGCGTG350.002166329416.28711915
CTTATAC851.0149961E-915.6453913
ACCGTTA551.125527E-515.5452968
GGACCGT551.125527E-515.5452966
TCCTAGA755.8271326E-815.198382
CCAACCC508.70402E-515.19837953
CTAGGAC1004.0017767E-1115.19837953
TCTAGGA1200.015.0400652
GCCCAAG456.752106E-414.7776287
TAGACTC456.752106E-414.7776285
CGCCTGT456.752106E-414.77762810
Produced by FastQC (version 0.11.5)