##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063948_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519220
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257655714340743	32.0	32.0	32.0	32.0	32.0
2	31.041279226532104	32.0	32.0	32.0	32.0	32.0
3	31.06324294133508	32.0	32.0	32.0	32.0	32.0
4	31.043261045414276	32.0	32.0	32.0	32.0	32.0
5	31.075214745194714	32.0	32.0	32.0	32.0	32.0
6	34.710132506451984	36.0	36.0	36.0	32.0	36.0
7	34.60723007588305	36.0	36.0	36.0	32.0	36.0
8	34.55140595508647	36.0	36.0	36.0	32.0	36.0
9	34.58500828165325	36.0	36.0	36.0	32.0	36.0
10	34.42554408535881	36.0	36.0	36.0	32.0	36.0
11	34.63293979430684	36.0	36.0	36.0	32.0	36.0
12	34.50648472709064	36.0	36.0	36.0	32.0	36.0
13	34.541175224375024	36.0	36.0	36.0	32.0	36.0
14	34.509375601864335	36.0	36.0	36.0	32.0	36.0
15	34.48988097530912	36.0	36.0	36.0	32.0	36.0
16	34.46862023804938	36.0	36.0	36.0	32.0	36.0
17	34.43795693540311	36.0	36.0	36.0	32.0	36.0
18	34.45865721659412	36.0	36.0	36.0	32.0	36.0
19	34.34776202765687	36.0	36.0	36.0	32.0	36.0
20	34.28775663495243	36.0	36.0	36.0	32.0	36.0
21	34.23119294326104	36.0	36.0	36.0	32.0	36.0
22	34.21680405223219	36.0	36.0	36.0	32.0	36.0
23	34.261020376718925	36.0	36.0	36.0	32.0	36.0
24	34.19226339509264	36.0	36.0	36.0	32.0	36.0
25	33.91598359077077	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	7.0
5	9.0
6	31.0
7	7.0
8	34.0
9	49.0
10	59.0
11	17.0
12	35.0
13	26.0
14	83.0
15	122.0
16	165.0
17	207.0
18	287.0
19	363.0
20	524.0
21	706.0
22	1046.0
23	1549.0
24	2371.0
25	3267.0
26	4523.0
27	6078.0
28	8581.0
29	11657.0
30	15592.0
31	21800.0
32	31479.0
33	46405.0
34	106844.0
35	255296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1331067489769	19.25967029664054	12.413393139272308	25.19382981511025
2	11.311832844744233	20.039408145891034	49.89444934531629	18.754309664048446
3	19.730508993626746	26.962968099059903	30.37609711845701	22.93042578885634
4	10.146998775010209	17.416427190150774	40.65887501251955	31.777699022319467
5	9.732241058168853	41.4692166784485	36.712696061530046	12.085846201852604
6	27.931185264662428	40.937871412528196	19.219159637318377	11.911783685491
7	25.653726040962272	32.37641673554077	22.19056260993367	19.779294613563287
8	22.353011449249113	44.40929042401914	19.00340278928526	14.234295337446481
9	28.185378036606938	13.622961623994541	20.559510365799603	37.632149973598914
10	16.478183780450202	28.9540934320074	34.73172987974098	19.83599290780142
11	33.97003131775476	19.685473379908455	30.026306913996624	16.31818838834016
12	26.87341838022862	23.433895280147865	34.9610967310461	14.731589608577414
13	31.97228432657467	23.2164058283864	26.60049340837252	18.21081643666641
14	18.586035753866913	26.559051703368187	27.673878475401146	27.181034067363758
15	19.698184481362986	40.456192421847895	23.274293643757467	16.571329453031648
16	17.08009050994723	27.473893585280955	36.47516180387713	18.97085410089469
17	17.032537091359035	31.63317619214654	31.694079644755558	19.64020707173887
18	16.816827236313028	26.916523059960134	40.37623029503949	15.89041940868734
19	25.389023494648217	23.534548061966195	28.09758692672885	22.97884151665674
20	23.785952068406516	28.724259369560567	31.278709127743692	16.21107943428923
21	21.984294041450777	24.418833271650627	27.59799839624969	25.998874290648903
22	22.849553564544234	32.640291763689916	27.836869862908074	16.673284808857773
23	18.625905049772918	32.57716537254509	31.86276399694654	16.934165580735453
24	24.26139638345898	27.474878706511202	31.83257739748604	16.43114751254378
25	20.21002839315557	30.4577793969394	32.70899232247531	16.623199887429717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	162.0
1	162.0
2	417.5
3	673.0
4	673.0
5	673.0
6	1874.0
7	3075.0
8	3075.0
9	3075.0
10	2674.0
11	2273.0
12	2273.0
13	2273.0
14	1818.0
15	1363.0
16	1363.0
17	1363.0
18	2858.5
19	4354.0
20	4354.0
21	4354.0
22	9324.5
23	14295.0
24	14295.0
25	14295.0
26	26064.5
27	37834.0
28	37834.0
29	37834.0
30	53737.0
31	69640.0
32	69640.0
33	69640.0
34	69283.5
35	68927.0
36	68927.0
37	68927.0
38	66857.5
39	64788.0
40	64788.0
41	64788.0
42	66258.5
43	67729.0
44	67729.0
45	67729.0
46	67603.5
47	67478.0
48	67478.0
49	67478.0
50	60097.5
51	52717.0
52	52717.0
53	52717.0
54	39174.0
55	25631.0
56	25631.0
57	25631.0
58	20992.0
59	16353.0
60	16353.0
61	16353.0
62	13405.0
63	10457.0
64	10457.0
65	10457.0
66	8297.5
67	6138.0
68	6138.0
69	6138.0
70	4468.0
71	2798.0
72	2798.0
73	2798.0
74	2012.5
75	1227.0
76	1227.0
77	1227.0
78	915.5
79	604.0
80	604.0
81	604.0
82	433.5
83	263.0
84	263.0
85	263.0
86	190.0
87	117.0
88	117.0
89	117.0
90	75.0
91	33.0
92	33.0
93	33.0
94	30.5
95	28.0
96	28.0
97	28.0
98	145.5
99	263.0
100	263.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04006009013520281
2	0.007318670313162051
3	0.0036593351565810256
4	0.006163090790031201
5	0.011363198644120026
6	0.016755903085397327
7	0.030237664188590578
8	0.045067601402103155
9	0.05816416933091946
10	0.06548283964408151
11	0.06644582258002388
12	0.07106814067254728
13	0.0731867031316205
14	0.07607565193944764
15	0.069334771387851
16	0.07357189630599746
17	0.07049035091098185
18	0.08512769153730596
19	0.08108316320634798
20	0.08454990177574054
21	0.08397211201417511
22	0.0855128847116829
23	0.08936481645545241
24	0.08416470860136359
25	0.08339432225260968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	519220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.751700057546696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.08416317770477	19.172029752342752
2	14.496935850209697	8.046292309370305
3	5.591299092384294	4.655041660316461
4	2.7635157966429578	3.067690459709103
5	1.598096352875707	2.2174945324032964
6	1.041475608012562	1.7341631214489421
7	0.7120282174050228	1.383199546535369
8	0.5376456654325421	1.193646499545921
9	0.42980177009292864	1.073495682703944
>10	2.9807708960427037	16.366139392578976
>50	0.4123296443950178	7.981483229994131
>100	0.3061235238690284	16.245570145506736
>500	0.027072148369369855	5.431500281658731
>1k	0.01804809891291324	10.443429447394328
>5k	6.94157650496663E-4	0.9888239384910184
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	5133	0.9885982820384424	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4404	0.848195369978044	No Hit
GTCCTACAGTGGACATTTCTAAATT	4344	0.8366395747467354	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4282	0.8246985863410501	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3975	0.7655714340741882	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3292	0.6340279650244598	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2867	0.5521744154693579	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2552	0.49150649050498824	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2412	0.4645429682986017	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2104	0.4052232194445515	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1960	0.37748931088941107	No Hit
GTACATGGGGTGGTATCAACGCAAA	1920	0.369785447401872	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1791	0.34494048765455876	No Hit
GATATACACTGTTCTACAAATCCCG	1609	0.3098879087862563	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1540	0.29659874427025157	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1506	0.29005046030584336	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1506	0.29005046030584336	No Hit
GAATATGGCAAGAAAACTGAAAATC	1463	0.28176880705673896	No Hit
TTGTAGAACAGTGTATATCAATGAG	1419	0.27329455722044604	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1418	0.27310196063325753	No Hit
GTATCAACGCAGAGTACATGGGGTG	1204	0.23188629097492391	No Hit
CTGTAGGACATGGAATATGGCAAGA	1181	0.22745656946958898	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1161	0.22360463772581948	No Hit
GTGTATATCAATGAGTTACAATGAA	1131	0.21782674011016526	No Hit
GTGTATATCAATGAGTTACAATGAG	1120	0.21570817765109201	No Hit
GCCATATTCCACGTCCTACAGTGGA	1042	0.20068564385039098	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1009	0.19432995647317128	No Hit
GTTCTACAGTGTGGTTTTTATCATT	980	0.18874465544470553	No Hit
CCATATTTCACGTCCTAAAGTGTGT	943	0.18161858171873194	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	919	0.17699626362620854	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	918	0.1768036670390201	No Hit
GATATACACTGTTCTACAATGCCGG	878	0.16909980355148108	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	878	0.16909980355148108	No Hit
ACAGTGGACATTTCTAAATTTTCCA	876	0.16871461037710414	No Hit
GTACATGGGTGGTATCAACGCAAAA	834	0.16062555371518816	No Hit
ATACACACTTTAGGACGTGAAATAT	830	0.15985516736643426	No Hit
GTGTAAAGGTATAAGGGAGCTTGAC	810	0.15600323562266477	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	798	0.15369207657640307	No Hit
GACCTGGAATATGGCGAGAAAACTG	785	0.1511883209429529	No Hit
GTCCACTGTAGGACGTGGAATATGG	781	0.150417934594199	No Hit
CTGTAGAACATATTAGATGAGTGAG	778	0.14984014483263355	No Hit
TATCAACGCAGAGTACATGGGGTGG	777	0.1496475482454451	No Hit
CCCTTATACCTTTACACTCTGCGAA	773	0.14887716189669117	No Hit
ACCTGGAATATGGCGAGAAAACTGA	761	0.1465660028504295	No Hit
CCTTTACACTCTGCGAATGATTTCC	758	0.14598821308886406	No Hit
GTGTATTTCTCATTTTCCGTGATTT	751	0.14464003697854472	No Hit
CCACTGTAGGACGTGGAATATGGCA	735	0.14155849158352915	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	728	0.1402103154732098	No Hit
GTTCTACAAATCCCGTTTCCAACGA	721	0.1388621393628905	No Hit
CACTTTAGGACGTGAAATATGGCGA	705	0.1357805939678749	No Hit
GAAATACACACTTTAGGACGTGAAA	683	0.13154346904972844	No Hit
GTAGGACGTGGAATATGGCAAGAAA	672	0.1294249065906552	No Hit
GTATCAACGCAGAGTACATGGGAAA	655	0.12615076460845115	No Hit
CTACAGTGGACATTTCTAAATTTTC	638	0.12287662262624706	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	625	0.12037286699279688	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	615	0.11844690112091213	No Hit
GTAGAACATATTAGATGAGTGAGTT	612	0.1178691113593467	No Hit
GGTATCAACGCAGAGTACATGGGGT	601	0.11575054890027349	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	585	0.1126690035052579	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	580	0.1117060205693155	No Hit
ATTCCACGTCCTACAGTGGACATTT	567	0.10920226493586535	No Hit
GTACATGGGAGTGGTATCAACGCAA	554	0.10669850930241516	No Hit
GTATTTCTCATTTTCCGTGATTTTC	543	0.10457994684334193	No Hit
GTATAAGGGAGCTTGACTGCGAGAG	521	0.10034282192519549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAATCG	35	9.924169E-5	18.999807	11
GAAAGCG	25	0.006027132	18.999807	9
TTATACC	70	1.3060344E-9	17.640978	4
CCTAGAG	65	9.995347E-9	17.536592	3
AGGCCCA	40	2.756882E-4	16.624832	5
GTTCCCT	40	2.756882E-4	16.624832	9
CCCTTAT	80	4.292815E-10	16.62323	1
ACTGTGC	75	3.265086E-9	16.4665	8
CCTTATA	75	3.268724E-9	16.464912	2
TCGCGTG	35	0.0021663294	16.287119	15
CTTATAC	85	1.0149961E-9	15.645391	3
ACCGTTA	55	1.125527E-5	15.545296	8
GGACCGT	55	1.125527E-5	15.545296	6
TCCTAGA	75	5.8271326E-8	15.19838	2
CCAACCC	50	8.70402E-5	15.1983795	3
CTAGGAC	100	4.0017767E-11	15.1983795	3
TCTAGGA	120	0.0	15.040065	2
GCCCAAG	45	6.752106E-4	14.777628	7
TAGACTC	45	6.752106E-4	14.777628	5
CGCCTGT	45	6.752106E-4	14.777628	10
>>END_MODULE