FastQCFastQC Report
Thu 2 Feb 2017
SRR4063948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519220
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22190.42737182697122605No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA19660.3786448904125419No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA18980.3655483224837256No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC15830.304880397519356No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13460.2592350063556874No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA11340.2184045298717307No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA10920.21031547320981472No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA10060.1937521667116059No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8330.16043295712799968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.15985516736643426No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA8030.15465505951234546No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG7790.15003274141982206No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT7480.14406224721697933No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA7450.14348445745541388No Hit
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG7400.1425214745194715No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC7360.1417510881707176No Hit
CCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAAC6810.13115827587535148No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA6720.1294249065906552No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC6670.12846192365471285No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG5810.111898617156504No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC5700.10978005469743075No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5520.1063133161280382No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA5400.10400215708177651No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA5350.10303917414583415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.10303917414583415No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA5220.10053541851238396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAG207.853927E-443.9983631
ATAGGAC1550.031.2216363
TATAGGA1450.030.3407762
GACCTAG300.005743077529.3322437
CCGTAAG703.583773E-828.284661
TAAGACC554.9590453E-627.9962624
GTACCTA407.0282206E-427.4989761
GCTCTAC407.0282206E-427.49897611
CAAGGAT407.0282206E-427.49897644
TAAGACT1006.002665E-1126.3964734
TAGGACC10450.025.891284
ACTGTTC7600.025.4727368
GTCCTAT1750.025.1419221
ATACACT7950.024.6256434
TAAGCTA450.001395875624.44824632
TATCGTT450.001397443424.44353713
GCGAAGA450.001398227824.44118120
AAGACCT901.5104888E-824.4411815
TCCTATA1800.024.4411812
ATCGTTA450.001398227824.44118114
Produced by FastQC (version 0.11.5)