##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063948_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519220
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.188186125341858	32.0	32.0	32.0	32.0	32.0
2	31.50422942105466	32.0	32.0	32.0	32.0	32.0
3	31.55863410500366	32.0	32.0	32.0	32.0	32.0
4	31.627762798043218	32.0	32.0	32.0	32.0	32.0
5	31.579344016024034	32.0	32.0	32.0	32.0	32.0
6	35.177308270097456	36.0	36.0	36.0	36.0	36.0
7	35.23354647355649	36.0	36.0	36.0	36.0	36.0
8	35.16985670813913	36.0	36.0	36.0	36.0	36.0
9	35.28036285197026	36.0	36.0	36.0	36.0	36.0
10	35.159670274642735	36.0	36.0	36.0	36.0	36.0
11	35.26154038750433	36.0	36.0	36.0	36.0	36.0
12	35.2183929740765	36.0	36.0	36.0	36.0	36.0
13	35.23589615192019	36.0	36.0	36.0	36.0	36.0
14	35.18732907052887	36.0	36.0	36.0	36.0	36.0
15	35.148923385077616	36.0	36.0	36.0	36.0	36.0
16	35.17722160163322	36.0	36.0	36.0	36.0	36.0
17	35.175014444744036	36.0	36.0	36.0	36.0	36.0
18	35.15324525249412	36.0	36.0	36.0	36.0	36.0
19	35.12482762605447	36.0	36.0	36.0	36.0	36.0
20	35.096602596202	36.0	36.0	36.0	36.0	36.0
21	35.06544432032664	36.0	36.0	36.0	36.0	36.0
22	35.02593313046493	36.0	36.0	36.0	36.0	36.0
23	35.05253842301914	36.0	36.0	36.0	36.0	36.0
24	35.022375871499555	36.0	36.0	36.0	36.0	36.0
25	34.967934594198994	36.0	36.0	36.0	36.0	36.0
26	34.94768691498787	36.0	36.0	36.0	36.0	36.0
27	34.866998574785256	36.0	36.0	36.0	36.0	36.0
28	34.801459882130885	36.0	36.0	36.0	32.0	36.0
29	34.758470397904546	36.0	36.0	36.0	32.0	36.0
30	34.698748122183275	36.0	36.0	36.0	32.0	36.0
31	34.68277993913948	36.0	36.0	36.0	32.0	36.0
32	34.61112245291014	36.0	36.0	36.0	32.0	36.0
33	34.654266014406225	36.0	36.0	36.0	32.0	36.0
34	34.585975116520935	36.0	36.0	36.0	32.0	36.0
35	34.55169292400139	36.0	36.0	36.0	32.0	36.0
36	34.45171603559185	36.0	36.0	36.0	32.0	36.0
37	34.34915642694811	36.0	36.0	36.0	32.0	36.0
38	34.27553252956358	36.0	36.0	36.0	32.0	36.0
39	34.0535553329995	36.0	36.0	36.0	32.0	36.0
40	34.155082623935904	36.0	36.0	36.0	32.0	36.0
41	34.09768691498787	36.0	36.0	36.0	32.0	36.0
42	34.07250876314472	36.0	36.0	36.0	32.0	36.0
43	34.05531373984053	36.0	36.0	36.0	32.0	36.0
44	33.88531836215862	36.0	36.0	36.0	32.0	36.0
45	33.82283039944532	36.0	36.0	36.0	32.0	36.0
46	33.57328107545934	36.0	36.0	36.0	21.0	36.0
47	33.54678941489157	36.0	36.0	36.0	21.0	36.0
48	33.416977389160664	36.0	36.0	36.0	21.0	36.0
49	33.33340780401371	36.0	36.0	36.0	21.0	36.0
50	32.91151727591387	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	4.0
21	13.0
22	39.0
23	140.0
24	365.0
25	956.0
26	2075.0
27	3917.0
28	6892.0
29	10456.0
30	14995.0
31	20936.0
32	29507.0
33	46314.0
34	91061.0
35	291546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.282193026216696	20.305562885589197	12.848125769252253	25.56411831894185
2	11.131376669099817	20.558071095514975	49.10759855705803	19.202953678327173
3	19.347134831352474	27.221461464237635	30.808194615374244	22.623209089035647
4	10.063364277185007	17.973113516428487	40.87824043757944	31.085281768807054
5	9.577635684295673	41.82947498170332	36.331612803821116	12.261276530179885
6	28.484897509528732	40.878319167827065	18.667267569176012	11.969515753468189
7	25.607449635992452	32.71233773737529	22.171719117137243	19.50849350949501
8	22.434613458649512	44.455722044605366	18.95901544624629	14.150649050498826
9	27.63036507034658	13.773484972506573	20.556802095471046	38.0393478616758
10	16.497789932879442	29.25801450266267	34.406170854077786	19.838024710380093
11	33.72289203035322	19.767150726089135	30.09475752089673	16.415199722660915
12	26.771413934031564	23.683886151866844	34.74629153842895	14.798408375672647
13	31.938091395312558	23.11538335609196	26.77146549515906	18.17505975343642
14	18.645083009129078	26.760140210315477	27.540926774777553	27.053850005777896
15	19.86236332153939	40.2852118576329	23.17750817133513	16.674916649492577
16	17.179236307187402	27.69301290026848	35.971680270562814	19.156070521981302
17	17.114517930742267	31.799429914101925	31.48010477254343	19.605947382612378
18	17.032762794746706	26.7997773570588	40.19745116203822	15.97000868615627
19	25.730711451793077	23.3355802935172	28.542621624744807	22.39108662994492
20	23.898347521281924	28.90720696429259	31.198143368899505	15.99630214552598
21	22.08986556758214	24.245213974808365	27.87681522283425	25.788105234775237
22	23.147606024421247	32.05847232387042	28.06767844073803	16.726243210970303
23	18.720748829953198	32.548872325263375	31.752662699100558	16.977716145682862
24	24.64090227996718	27.31338281800708	31.438625009148375	16.607089892877365
25	20.495560563163266	30.332235511642686	32.50881146356966	16.66339246162439
26	15.837777764937238	32.09235942980302	34.57353680451627	17.496326000743466
27	18.259229868408887	31.12807790686924	32.20245616197725	18.410236062744616
28	16.48060832986641	32.6310883064454	29.706351407571763	21.181951956116425
29	20.367791651499306	27.336688449886076	28.273886908304398	24.021632990310223
30	16.623876394214935	29.903370383995348	31.945169385919463	21.527583835870253
31	22.928713138278408	26.215305198682515	31.19286553537377	19.66311612766531
32	18.533163584675624	32.22309750211384	30.494280613021214	18.749458300189332
33	16.824444183354487	32.16793049186138	33.87077202802052	17.13685329676362
34	18.91296894802328	31.056021448629995	33.26771881030982	16.7632907930369
35	17.570992742792868	32.78262276390194	32.1429947150275	17.503389778277683
36	16.12053224029401	31.32632462959681	33.72537387029917	18.82776925981
37	17.599349770127564	30.852791115579514	28.263042636666725	23.2848164776262
38	19.34607161430745	34.84369281738159	28.329448928096767	17.480786640214188
39	22.931758080266256	28.273083242006408	29.090114157054064	19.70504452067327
40	23.944114990302555	29.26682703882252	30.835752076716698	15.953305894158229
41	22.951552201664878	26.770711751586102	33.06869440945458	17.20904163729444
42	19.99861325382655	34.21025768440353	30.24223759199231	15.548891469777601
43	16.191651964900657	34.311032872892	32.19898615594401	17.298329006263337
44	15.770679732821783	33.2326022141586	30.442562075792946	20.554155977226678
45	16.579899731706238	33.68926073801008	30.267696699004443	19.46314283127924
46	19.357292810230927	28.51986672059475	29.77138344793051	22.35145702124381
47	23.427445527086707	29.509652293996762	28.17568465513489	18.88721752378164
48	17.415187152591862	31.583468476623743	27.97215727212145	23.02918709866295
49	21.385360592432807	29.442716408424257	28.818119661411938	20.353803337730998
50	17.63989869321957	35.46045472492128	26.596689232784104	20.302957349075047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39.0
1	30.0
2	21.0
3	54.5
4	88.0
5	99.0
6	110.0
7	121.5
8	133.0
9	223.0
10	313.0
11	472.0
12	631.0
13	1215.5
14	1800.0
15	2520.5
16	3241.0
17	3870.5
18	4500.0
19	4697.5
20	4895.0
21	4957.0
22	5019.0
23	5335.0
24	5651.0
25	5775.0
26	5899.0
27	9331.0
28	12763.0
29	17229.0
30	21695.0
31	27100.0
32	32505.0
33	42042.5
34	51580.0
35	60877.0
36	70174.0
37	67403.5
38	64633.0
39	55375.5
40	46118.0
41	39127.0
42	32136.0
43	26931.0
44	21726.0
45	20062.0
46	18398.0
47	17432.0
48	16466.0
49	16369.0
50	16272.0
51	18011.5
52	19751.0
53	17106.0
54	14461.0
55	13201.5
56	11942.0
57	10880.5
58	9819.0
59	9190.5
60	8562.0
61	7407.5
62	6253.0
63	5026.0
64	3799.0
65	3390.5
66	2982.0
67	2192.5
68	1403.0
69	1255.5
70	1108.0
71	970.5
72	833.0
73	712.0
74	591.0
75	486.5
76	382.0
77	320.0
78	258.0
79	191.5
80	125.0
81	97.0
82	69.0
83	50.5
84	32.0
85	24.0
86	16.0
87	11.0
88	6.0
89	8.0
90	10.0
91	6.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	2.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009052039597858327
2	5.777897615654251E-4
3	1.9259658718847502E-4
4	0.0
5	0.0
6	1.9259658718847502E-4
7	0.0
8	0.0
9	9.629829359423751E-4
10	9.629829359423751E-4
11	0.0
12	3.8519317437695004E-4
13	5.777897615654251E-4
14	0.0
15	0.0052001078540888255
16	0.0011555795231308502
17	0.0
18	5.777897615654251E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0019259658718847501
24	3.8519317437695004E-4
25	0.0019259658718847501
26	0.005970494202842726
27	0.006933477138785101
28	0.005392704441277301
29	0.0025037556334501754
30	0.004044528330957975
31	0.00962982935942375
32	0.004044528330957975
33	0.005585301028465776
34	0.005007511266900351
35	0.0023111590462617003
36	0.010785408882554602
37	0.0036593351565810256
38	0.00962982935942375
39	0.004429721505334925
40	0.0021185624590732252
41	0.0042371249181464505
42	0.0036593351565810256
43	0.0015407726975078001
44	0.0030815453950156003
45	0.0025037556334501754
46	0.0019259658718847501
47	0.0032741419822040754
48	0.0034667385693925505
49	9.629829359423751E-4
50	9.629829359423751E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	519220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.82634514927528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41636492632891	31.86980929089385
2	13.208654435341959	11.313567876109209
3	4.649971636311963	5.974238706931093
4	2.253352561722906	3.860114182053951
5	1.255993941712164	2.6894815026581935
6	0.7938578043180397	2.039881699630212
7	0.5103537389762076	1.5299609761532744
8	0.408692794044648	1.4002254926222215
9	0.28451286013542765	1.0966181352810573
>10	1.8431671534645917	15.392545026719533
>50	0.21394349215870628	6.4109862869589485
>100	0.14894800086804272	11.625445856193203
>500	0.008575793989372157	2.430360326392823
>1k	0.003610860627104066	2.366764641402507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2219	0.42737182697122605	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1966	0.3786448904125419	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1898	0.3655483224837256	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1583	0.304880397519356	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1346	0.2592350063556874	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1134	0.2184045298717307	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1092	0.21031547320981472	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1006	0.1937521667116059	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	833	0.16043295712799968	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	830	0.15985516736643426	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	803	0.15465505951234546	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	779	0.15003274141982206	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	748	0.14406224721697933	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	745	0.14348445745541388	No Hit
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG	740	0.1425214745194715	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	736	0.1417510881707176	No Hit
CCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAAC	681	0.13115827587535148	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	672	0.1294249065906552	No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC	667	0.12846192365471285	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	581	0.111898617156504	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	570	0.10978005469743075	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	552	0.1063133161280382	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	540	0.10400215708177651	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	535	0.10303917414583415	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	535	0.10303917414583415	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	522	0.10053541851238396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.9259658718847502E-4	0.0
16	0.0	0.0	0.0	1.9259658718847502E-4	0.0
17	0.0	0.0	0.0	1.9259658718847502E-4	0.0
18	0.0	0.0	0.0	1.9259658718847502E-4	0.0
19	0.0	0.0	0.0	1.9259658718847502E-4	0.0
20	0.0	0.0	0.0	5.777897615654251E-4	0.0
21	0.0	0.0	0.0	0.001348176110319325	0.0
22	0.0	0.0	0.0	0.0017333692846962752	0.0
23	0.0	0.0	0.0	0.005970494202842726	0.0
24	0.0	0.0	0.0	0.009822425946612225	0.0
25	0.0	0.0	0.0	0.010978005469743077	0.0
26	0.0	0.0	0.0	0.013096567928816302	0.0
27	0.0	0.0	0.0	0.014637340626324102	0.0
28	0.0	0.0	0.0	0.018489272370093603	0.0
29	0.0	0.0	0.0	0.02407457339855938	0.0
30	0.0	0.0	0.0	0.044297215053349256	0.0
31	0.0	0.0	0.0	0.08243133931666731	0.0
32	0.0	0.0	0.0	0.12268402603905859	0.0
33	0.0	0.0	0.0	0.1565810253842302	0.0
34	0.0	0.0	0.0	0.19317437695004044	0.0
35	0.0	0.0	0.0	0.23535302954431647	0.0
36	0.0	0.0	0.0	0.2831169831670583	0.0
37	0.0	0.0	0.0	0.37363737914564155	0.0
38	0.0	0.0	0.0	0.47590616694272175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCGAG	20	7.853927E-4	43.998363	1
ATAGGAC	155	0.0	31.221636	3
TATAGGA	145	0.0	30.340776	2
GACCTAG	30	0.0057430775	29.332243	7
CCGTAAG	70	3.583773E-8	28.28466	1
TAAGACC	55	4.9590453E-6	27.996262	4
GTACCTA	40	7.0282206E-4	27.498976	1
GCTCTAC	40	7.0282206E-4	27.498976	11
CAAGGAT	40	7.0282206E-4	27.498976	44
TAAGACT	100	6.002665E-11	26.396473	4
TAGGACC	1045	0.0	25.89128	4
ACTGTTC	760	0.0	25.472736	8
GTCCTAT	175	0.0	25.141922	1
ATACACT	795	0.0	24.625643	4
TAAGCTA	45	0.0013958756	24.448246	32
TATCGTT	45	0.0013974434	24.443537	13
GCGAAGA	45	0.0013982278	24.441181	20
AAGACCT	90	1.5104888E-8	24.441181	5
TCCTATA	180	0.0	24.441181	2
ATCGTTA	45	0.0013982278	24.441181	14
>>END_MODULE