FastQCFastQC Report
Thu 2 Feb 2017
SRR4063947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548941
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT64811.1806368990474385No Hit
CTTTAGGACGTGAAATATGGCGAGG54140.9862626402473126No Hit
GTCCTACAGTGGACATTTCTAAATT52220.9512862037996797No Hit
CTGTAGGACGTGGAATATGGCAAGA52180.9505575280403541No Hit
GTCCTACAGTGTGCATTTCTCATTT33660.6131806514725626No Hit
GTATCAACGCAGAGTACTTTTTTTT29820.543227778577297No Hit
ATTTAGAAATGTCCACTGTAGGACG27600.5027862739347215No Hit
CTGAAGGACCTGGAATATGGCGAGA26650.4854802246507366No Hit
CTGTAGGACCTGGAATATGGCGAGA25510.46471296550995467No Hit
GTCCTTCAGTGTGCATTTCTCATTT25390.4625269382319776No Hit
TATCAACGCAGAGTACTTTTTTTTT23880.435019428317433No Hit
GATATACACTGTTCTACAAATCCCG23770.4330155699792874No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23150.42172109570973937No Hit
TTTCTAAATTTTCCACCTTTTTCAG20660.37636102969171553No Hit
TTGTAGAACAGTGTATATCAATGAG20320.37016728573744717No Hit
GTGTATATCAATGAGTTACAATGAG16440.29948573708285586No Hit
GAATATGGCAAGAAAACTGAAAATC16060.2925633173692619No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15110.2752572680852769No Hit
GTACATGGGGTGGTATCAACGCAAA15050.2741642544462884No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14690.26760617261235725No Hit
GGAATATGGCGAGAAAACTGAAAAT14140.2575868809216291No Hit
GTTCTACAGTGTGGTTTTTATCATT13860.25248615060634927No Hit
ATTCCAGGTCCTTCAGTGTGCATTT12980.23645528390118428No Hit
CCATATTCCAGGTCCTTCAGTGTGC12630.23007937100708456No Hit
GTGTATATCAATGAGTTACAATGAA12290.22388562705281623No Hit
GCCATATTCCACGTCCTACAGTGGA11920.21714537627905367No Hit
GAAATATGGCGAGGAAAACTGAAAA11710.21331982854259382No Hit
CTGTAGGACATGGAATATGGCAAGA11550.2104051255052911No Hit
GTTCTACAAATCCCGTTTCCAACGA11300.2058509020095056No Hit
TTCCAGGTCCTTCAGTGTGCATTTC11170.2034827057916971No Hit
GGTATCAACGCAGAGTACTTTTTTT11030.2009323406340572No Hit
GTCCACTGTAGGACGTGGAATATGG10480.19091304894332906No Hit
ACCTGGAATATGGCGAGAAAACTGA10390.18927352848484627No Hit
GTATCAACGCAGAGTACATGGGGTG10310.1878161769661949No Hit
ACAGTGGACATTTCTAAATTTTCCA10310.1878161769661949No Hit
CCATATTTCACGTCCTAAAGTGTGT10290.18745183908653207No Hit
GATATACACTGTTCTACAATGCCGG10130.18453713604922933No Hit
ATACACACTTTAGGACGTGAAATAT9780.1781612231551296No Hit
GACCTGGAATATGGCGAGAAAACTG9750.17761471633563536No Hit
CCATAGGGTCTTCTCGTCTTATTAT9240.16832410040423287No Hit
CACTTTAGGACGTGAAATATGGCGA8770.1597621602321561No Hit
CCTAAAGTGTGTATTTCTCATTTTC8670.15794047083384188No Hit
CCACTGTAGGACGTGGAATATGGCA8640.15739396401434763No Hit
GTATTAGAGGCACTGCCTGCCCAGT8630.15721179507451621No Hit
GTGTATTTCTCATTTTCCGTGATTT8590.15648311931519054No Hit
GAAATACACACTTTAGGACGTGAAA8590.15648311931519054No Hit
AGTGTGTATTTCTCATTTTCCGTGA7900.1439134624668225No Hit
CTACAGTGGACATTTCTAAATTTTC7890.14373129352699107No Hit
CTCTAATACTTGTAATGCTAGAGGT7790.14190960412867687No Hit
GTAGGACGTGGAATATGGCAAGAAA7610.13863056321171127No Hit
ATTCCACGTCCTACAGTGGACATTT7340.13371200183626292No Hit
TTTCTAAATATTCCACCTTTTTCAG7260.13225465031761155No Hit
TCCTAAAGTGTGTATTTCTCATTTT7230.1317081434981173No Hit
TATCAACGCAGAGTACATGGGGTGG7060.12861127152098312No Hit
CTGTAGAACAGATTAGATGAGTGAG6880.12533223060401755No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6870.12515006166418613No Hit
CTGTAGAACATATTAGATGAGTGAG6720.12241752756671483No Hit
GTCCTACAGTGGACATTTCTAAATA6720.12241752756671483No Hit
ATATTTCACGTCCTAAAGTGTGTAT6560.11950282452941208No Hit
TTGATATACACTGTTCTACAAATCC6520.11877414877008641No Hit
GTAGAACAGTGTATATCAATGAGTT6450.11749896619126646No Hit
CCATTGGGATGTCCTGATCCAACAT6410.11677029043194079No Hit
GTACATGGGTGGTATCAACGCAAAA6260.11403775633446946No Hit
CCTTCAGTGTGCATTTCTCATTTTT6000.10930136389885252No Hit
GGTCCTTCAGTGTGCATTTCTCATT6000.10930136389885252No Hit
GTATTTCTCATTTTCCGTGATTTTC5980.10893702601918967No Hit
CACTGAAGGACCTGGAATATGGCGA5890.1072975055607069No Hit
ACGTGGAATATGGCAAGAAAACTGA5850.10656882980138119No Hit
ATGTTCTACAGTGTGGTTTTTATCA5830.10620449192171835No Hit
GTTATATAATTTAAGCTCCATAGGG5640.10274328206492138No Hit
TTTTTCAAGTCGTCAAGTGGATGTT5630.10256111312508995No Hit
GTGCATTTCTCATTTTTCACGTTTT5590.10183243736576428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC250.0060218619.002928
AGAGTGC250.0060218619.002928
GTATTAT307.729725E-418.9942551
ATTGAAC453.5274974E-516.8914838
AACGCTT402.7499677E-416.63058918
ATATGCC402.7518827E-416.6290715
GAACGCT402.7518827E-416.6290717
GTTGAGC402.753799E-416.6275548
TTGTCCA402.753799E-416.6275549
TGAACTT402.753799E-416.62755410
GTCCACC402.753799E-416.62755411
AGAACCT402.7614753E-416.6214895
AACCTTG804.311005E-1016.6214896
GTTTAAA402.7633974E-416.6199741
CGTTCAA350.002165566416.28821814
ACGTGCA350.002165566416.2882188
TGCAATA350.002165566416.28821811
AGCGTTC350.002166886816.28673212
GACGTGC350.002166886816.2867327
AGGCATA350.002170851516.2822765
Produced by FastQC (version 0.11.5)