##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063947_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548941
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234788438101727	32.0	32.0	32.0	32.0	32.0
2	31.041170180401902	32.0	32.0	32.0	32.0	32.0
3	31.067460801798372	32.0	32.0	32.0	32.0	32.0
4	31.048081669979105	32.0	32.0	32.0	32.0	32.0
5	31.111358051229548	32.0	32.0	32.0	32.0	32.0
6	34.73368722686045	36.0	36.0	36.0	32.0	36.0
7	34.62685607378571	36.0	36.0	36.0	32.0	36.0
8	34.58459470143421	36.0	36.0	36.0	32.0	36.0
9	34.59537363760404	36.0	36.0	36.0	32.0	36.0
10	34.441929096205236	36.0	36.0	36.0	32.0	36.0
11	34.621119573870416	36.0	36.0	36.0	32.0	36.0
12	34.50693061731589	36.0	36.0	36.0	32.0	36.0
13	34.55028318161697	36.0	36.0	36.0	32.0	36.0
14	34.531727453405736	36.0	36.0	36.0	32.0	36.0
15	34.51729420830289	36.0	36.0	36.0	32.0	36.0
16	34.52159703866172	36.0	36.0	36.0	32.0	36.0
17	34.468809944966765	36.0	36.0	36.0	32.0	36.0
18	34.503999519074	36.0	36.0	36.0	32.0	36.0
19	34.37426973026245	36.0	36.0	36.0	32.0	36.0
20	34.326987781929205	36.0	36.0	36.0	32.0	36.0
21	34.29546709027017	36.0	36.0	36.0	32.0	36.0
22	34.26395915043693	36.0	36.0	36.0	32.0	36.0
23	34.307938375891034	36.0	36.0	36.0	32.0	36.0
24	34.233740966697695	36.0	36.0	36.0	32.0	36.0
25	33.92505023308516	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	3.0
5	3.0
6	17.0
7	6.0
8	30.0
9	36.0
10	56.0
11	11.0
12	39.0
13	32.0
14	65.0
15	122.0
16	189.0
17	242.0
18	304.0
19	408.0
20	560.0
21	766.0
22	1232.0
23	1642.0
24	2436.0
25	3505.0
26	4919.0
27	6497.0
28	8964.0
29	12138.0
30	16267.0
31	22405.0
32	32077.0
33	46561.0
34	110252.0
35	277155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56574575767682	20.798910874307676	12.571419198574077	25.06392416944142
2	10.441765604988786	20.80719825254003	50.77671972323382	17.97431641923736
3	19.075586775775506	27.470980012096213	31.251411832430975	22.202021379697303
4	9.497836680785172	18.037254634057476	41.85562690713668	30.609281778020677
5	8.730290305244077	42.815787795702356	37.97505943651451	10.478862462539055
6	27.344063139944176	41.92276579773938	19.668923399820727	11.064247662495719
7	24.75124310122096	33.214717152337066	23.199393618048607	18.834646128393363
8	20.858390372265596	46.175475810390196	19.93082434924592	13.035309468098289
9	26.77830673558509	14.139151545196732	21.91744370001531	37.16509801920287
10	15.575585793315897	29.392561267920126	36.54420677524311	18.487646163520864
11	32.823777722692874	19.8823289249209	31.591111240722647	15.702782111663579
12	26.486425397103165	24.004462283858125	35.788500869489134	13.72061144954958
13	30.690288135006206	23.700275429869286	27.650169432206155	17.959267002918352
14	17.121935480342778	27.76802527265999	28.3529932899119	26.757045957085328
15	18.47808651651805	42.811495191501464	22.977076695369405	15.733341596611083
16	16.15645142473824	27.51662437658588	38.30588123778691	18.021042960888966
17	15.782470402741449	32.6994340293284	33.23259480692289	18.285500761007263
18	15.96488829336323	27.44200461228545	41.78347780907326	14.809629285278058
19	23.99858535882847	24.579937252642882	28.808365342020494	22.613112046508153
20	22.96940572507812	30.66500891467074	31.55155377788797	14.814031582363171
21	20.143795710921037	26.247456959726996	27.999693741386476	25.609053587965498
22	21.875501334461184	34.393002464742516	28.385921799116193	15.345574401680109
23	17.716476576629134	33.72888833790327	32.067484394142696	16.487150691324892
24	23.761986363802095	28.35545265614176	32.75713712764794	15.12542385240821
25	19.91497228030708	31.37402534424012	33.674185591593485	15.036816783859317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	171.0
2	387.5
3	604.0
4	604.0
5	604.0
6	1928.5
7	3253.0
8	3253.0
9	3253.0
10	2908.5
11	2564.0
12	2564.0
13	2564.0
14	2429.0
15	2294.0
16	2294.0
17	2294.0
18	4669.0
19	7044.0
20	7044.0
21	7044.0
22	13142.0
23	19240.0
24	19240.0
25	19240.0
26	33837.5
27	48435.0
28	48435.0
29	48435.0
30	66590.5
31	84746.0
32	84746.0
33	84746.0
34	84322.0
35	83898.0
36	83898.0
37	83898.0
38	79502.0
39	75106.0
40	75106.0
41	75106.0
42	70292.0
43	65478.0
44	65478.0
45	65478.0
46	66041.5
47	66605.0
48	66605.0
49	66605.0
50	56433.0
51	46261.0
52	46261.0
53	46261.0
54	32206.0
55	18151.0
56	18151.0
57	18151.0
58	13985.0
59	9819.0
60	9819.0
61	9819.0
62	8141.0
63	6463.0
64	6463.0
65	6463.0
66	5287.0
67	4111.0
68	4111.0
69	4111.0
70	3185.5
71	2260.0
72	2260.0
73	2260.0
74	1713.5
75	1167.0
76	1167.0
77	1167.0
78	889.0
79	611.0
80	611.0
81	611.0
82	431.5
83	252.0
84	252.0
85	252.0
86	182.0
87	112.0
88	112.0
89	112.0
90	75.0
91	38.0
92	38.0
93	38.0
94	27.0
95	16.0
96	16.0
97	16.0
98	129.0
99	242.0
100	242.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044449221318866694
2	0.005465068194942625
3	0.0016395204584827878
4	0.002914703037302734
5	0.006558081833931151
6	0.011840981089042356
7	0.020402921261119138
8	0.03515860538746422
9	0.0497321205739779
10	0.059387074385043204
11	0.053375499370606316
12	0.062483946362177356
13	0.06266611530200877
14	0.06630949409863719
15	0.05993358120453746
16	0.06357696000116589
17	0.059387074385043204
18	0.07396058957155688
19	0.07141022441391698
20	0.0741427585113883
21	0.07013504183509703
22	0.07377842063172545
23	0.07815047518767955
24	0.07304974487239976
25	0.07541794109020823
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	548941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.220696678504467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.79831968900243	17.351415528671122
2	14.004542451766794	7.064086345944992
3	5.3973388395151956	4.083739372275722
4	2.796308782908292	2.8209942253267215
5	1.682374570303832	2.1215329368631117
6	1.1345227074285968	1.7168071847359387
7	0.8433610970087204	1.4889108092675403
8	0.5898531077573962	1.1901205052497998
9	0.4892667034174745	1.1105682409605537
>10	3.4685254800377847	17.342571580580042
>50	0.39454156919710576	6.914582730445231
>100	0.3432367131293502	17.25665966008087
>500	0.031071955083288548	5.366588062081144
>1k	0.023845919017407492	10.10096591642094
>5k	0.0028904144263524234	4.0704569010962866
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	6481	1.1806368990474385	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5414	0.9862626402473126	No Hit
GTCCTACAGTGGACATTTCTAAATT	5222	0.9512862037996797	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5218	0.9505575280403541	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3366	0.6131806514725626	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2982	0.543227778577297	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2760	0.5027862739347215	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2665	0.4854802246507366	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2551	0.46471296550995467	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2539	0.4625269382319776	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2388	0.435019428317433	No Hit
GATATACACTGTTCTACAAATCCCG	2377	0.4330155699792874	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2315	0.42172109570973937	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2066	0.37636102969171553	No Hit
TTGTAGAACAGTGTATATCAATGAG	2032	0.37016728573744717	No Hit
GTGTATATCAATGAGTTACAATGAG	1644	0.29948573708285586	No Hit
GAATATGGCAAGAAAACTGAAAATC	1606	0.2925633173692619	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1511	0.2752572680852769	No Hit
GTACATGGGGTGGTATCAACGCAAA	1505	0.2741642544462884	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1469	0.26760617261235725	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1414	0.2575868809216291	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1386	0.25248615060634927	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1298	0.23645528390118428	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1263	0.23007937100708456	No Hit
GTGTATATCAATGAGTTACAATGAA	1229	0.22388562705281623	No Hit
GCCATATTCCACGTCCTACAGTGGA	1192	0.21714537627905367	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1171	0.21331982854259382	No Hit
CTGTAGGACATGGAATATGGCAAGA	1155	0.2104051255052911	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1130	0.2058509020095056	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1117	0.2034827057916971	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1103	0.2009323406340572	No Hit
GTCCACTGTAGGACGTGGAATATGG	1048	0.19091304894332906	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1039	0.18927352848484627	No Hit
GTATCAACGCAGAGTACATGGGGTG	1031	0.1878161769661949	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1031	0.1878161769661949	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1029	0.18745183908653207	No Hit
GATATACACTGTTCTACAATGCCGG	1013	0.18453713604922933	No Hit
ATACACACTTTAGGACGTGAAATAT	978	0.1781612231551296	No Hit
GACCTGGAATATGGCGAGAAAACTG	975	0.17761471633563536	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	924	0.16832410040423287	No Hit
CACTTTAGGACGTGAAATATGGCGA	877	0.1597621602321561	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	867	0.15794047083384188	No Hit
CCACTGTAGGACGTGGAATATGGCA	864	0.15739396401434763	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	863	0.15721179507451621	No Hit
GTGTATTTCTCATTTTCCGTGATTT	859	0.15648311931519054	No Hit
GAAATACACACTTTAGGACGTGAAA	859	0.15648311931519054	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	790	0.1439134624668225	No Hit
CTACAGTGGACATTTCTAAATTTTC	789	0.14373129352699107	No Hit
CTCTAATACTTGTAATGCTAGAGGT	779	0.14190960412867687	No Hit
GTAGGACGTGGAATATGGCAAGAAA	761	0.13863056321171127	No Hit
ATTCCACGTCCTACAGTGGACATTT	734	0.13371200183626292	No Hit
TTTCTAAATATTCCACCTTTTTCAG	726	0.13225465031761155	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	723	0.1317081434981173	No Hit
TATCAACGCAGAGTACATGGGGTGG	706	0.12861127152098312	No Hit
CTGTAGAACAGATTAGATGAGTGAG	688	0.12533223060401755	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	687	0.12515006166418613	No Hit
CTGTAGAACATATTAGATGAGTGAG	672	0.12241752756671483	No Hit
GTCCTACAGTGGACATTTCTAAATA	672	0.12241752756671483	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	656	0.11950282452941208	No Hit
TTGATATACACTGTTCTACAAATCC	652	0.11877414877008641	No Hit
GTAGAACAGTGTATATCAATGAGTT	645	0.11749896619126646	No Hit
CCATTGGGATGTCCTGATCCAACAT	641	0.11677029043194079	No Hit
GTACATGGGTGGTATCAACGCAAAA	626	0.11403775633446946	No Hit
CCTTCAGTGTGCATTTCTCATTTTT	600	0.10930136389885252	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	600	0.10930136389885252	No Hit
GTATTTCTCATTTTCCGTGATTTTC	598	0.10893702601918967	No Hit
CACTGAAGGACCTGGAATATGGCGA	589	0.1072975055607069	No Hit
ACGTGGAATATGGCAAGAAAACTGA	585	0.10656882980138119	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	583	0.10620449192171835	No Hit
GTTATATAATTTAAGCTCCATAGGG	564	0.10274328206492138	No Hit
TTTTTCAAGTCGTCAAGTGGATGTT	563	0.10256111312508995	No Hit
GTGCATTTCTCATTTTTCACGTTTT	559	0.10183243736576428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.8216893983142085E-4	0.0
3	0.0	0.0	0.0	1.8216893983142085E-4	0.0
4	0.0	0.0	0.0	1.8216893983142085E-4	0.0
5	0.0	0.0	0.0	1.8216893983142085E-4	0.0
6	0.0	0.0	0.0	1.8216893983142085E-4	0.0
7	0.0	0.0	0.0	1.8216893983142085E-4	0.0
8	0.0	0.0	0.0	1.8216893983142085E-4	0.0
9	0.0	0.0	0.0	1.8216893983142085E-4	0.0
10	0.0	0.0	0.0	1.8216893983142085E-4	0.0
11	0.0	0.0	0.0	1.8216893983142085E-4	0.0
12	0.0	0.0	0.0	1.8216893983142085E-4	0.0
13	0.0	0.0	0.0	1.8216893983142085E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGCCC	25	0.00602186	19.00292	8
AGAGTGC	25	0.00602186	19.00292	8
GTATTAT	30	7.729725E-4	18.994255	1
ATTGAAC	45	3.5274974E-5	16.891483	8
AACGCTT	40	2.7499677E-4	16.630589	18
ATATGCC	40	2.7518827E-4	16.62907	15
GAACGCT	40	2.7518827E-4	16.62907	17
GTTGAGC	40	2.753799E-4	16.627554	8
TTGTCCA	40	2.753799E-4	16.627554	9
TGAACTT	40	2.753799E-4	16.627554	10
GTCCACC	40	2.753799E-4	16.627554	11
AGAACCT	40	2.7614753E-4	16.621489	5
AACCTTG	80	4.311005E-10	16.621489	6
GTTTAAA	40	2.7633974E-4	16.619974	1
CGTTCAA	35	0.0021655664	16.288218	14
ACGTGCA	35	0.0021655664	16.288218	8
TGCAATA	35	0.0021655664	16.288218	11
AGCGTTC	35	0.0021668868	16.286732	12
GACGTGC	35	0.0021668868	16.286732	7
AGGCATA	35	0.0021708515	16.282276	5
>>END_MODULE