FastQCFastQC Report
Thu 2 Feb 2017
SRR4063947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548941
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA29820.543227778577297No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA24670.44941077456411527No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA23010.4191707305520994No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC20530.37399283347390705No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG16540.3013074264811701No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA14700.26778834155218867No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA14410.26250544229707745No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC11730.21368416642225668No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10990.20020366487473154No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA9820.17888989891445528No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT9740.1774325473958039No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA8990.16376987690844735No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT8750.15939782235249325No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG8690.15830480871350472No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA8540.1555722746160334No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA8040.1464638276244624No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA7370.13425850865575717No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA7300.13298332607693725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.13225465031761155No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC7230.1317081434981173No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG7190.1309794677387916No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC7130.12988645409980307No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6800.1238748790853662No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT6770.12332837226587193No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT6730.12259969650654624No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA6680.12168885180738914No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTCTCATTGTAACTCAT6080.11075871541750387No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA6050.11021220859800962No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG6010.10948353283868394No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC5920.10784401238020115No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA5850.10656882980138119No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG5720.10420063358357273No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATG5610.10219677524542711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGA207.846637E-444.0071079
TCTAGCG207.8501593E-444.00309828
CTGTATG254.4427215E-543.9950831
GTGCACT301.299671E-436.66924711
TATACTC301.3003679E-436.665915
TAGCGGC250.002349814235.20568530
CTGTTCA250.002349814235.2056859
CGAGTAA250.00235086535.2024821
TGTGCAC353.2125463E-431.43364710
TAGGAAC701.033186E-931.4279214
CTAGGTA353.2159817E-431.4279214
GTATAGT353.2177003E-431.4250581
TAGGACC9500.030.1046414
CCGGTCC300.005737785229.338079
TGGCTAA300.005740341329.33539826
CTAGCGG300.005740341329.33539829
AGAACGT602.9409603E-729.3327275
GTGGTAC300.00574545529.3300531
ACTAGAG554.9588853E-627.996872
GGCGGGT407.0205756E-427.50444232
Produced by FastQC (version 0.11.5)