##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063947_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548941
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.250006831335245	32.0	32.0	32.0	32.0	32.0
2	31.498115462317443	32.0	32.0	32.0	32.0	32.0
3	31.54976217844905	32.0	32.0	32.0	32.0	32.0
4	31.616379538056	32.0	32.0	32.0	32.0	32.0
5	31.577867202486242	32.0	32.0	32.0	32.0	32.0
6	35.15306927338275	36.0	36.0	36.0	36.0	36.0
7	35.20154624996129	36.0	36.0	36.0	36.0	36.0
8	35.13375025731363	36.0	36.0	36.0	36.0	36.0
9	35.24452354624632	36.0	36.0	36.0	36.0	36.0
10	35.137473790443785	36.0	36.0	36.0	36.0	36.0
11	35.239207856582034	36.0	36.0	36.0	36.0	36.0
12	35.1801359344629	36.0	36.0	36.0	36.0	36.0
13	35.220644841613215	36.0	36.0	36.0	36.0	36.0
14	35.16503412935088	36.0	36.0	36.0	36.0	36.0
15	35.1305404405938	36.0	36.0	36.0	36.0	36.0
16	35.152285946941475	36.0	36.0	36.0	36.0	36.0
17	35.161234085265995	36.0	36.0	36.0	36.0	36.0
18	35.16747701483402	36.0	36.0	36.0	36.0	36.0
19	35.10905543583008	36.0	36.0	36.0	36.0	36.0
20	35.08745566463427	36.0	36.0	36.0	36.0	36.0
21	35.04779202136477	36.0	36.0	36.0	36.0	36.0
22	35.0241993219672	36.0	36.0	36.0	36.0	36.0
23	35.053931114637095	36.0	36.0	36.0	36.0	36.0
24	35.021102449990074	36.0	36.0	36.0	36.0	36.0
25	34.9835792917636	36.0	36.0	36.0	36.0	36.0
26	34.95161957295957	36.0	36.0	36.0	36.0	36.0
27	34.87670623983269	36.0	36.0	36.0	36.0	36.0
28	34.81423504529631	36.0	36.0	36.0	32.0	36.0
29	34.76951257056769	36.0	36.0	36.0	32.0	36.0
30	34.69490163788094	36.0	36.0	36.0	32.0	36.0
31	34.68307705199648	36.0	36.0	36.0	32.0	36.0
32	34.612331744212945	36.0	36.0	36.0	32.0	36.0
33	34.663306256956574	36.0	36.0	36.0	32.0	36.0
34	34.6189809105168	36.0	36.0	36.0	32.0	36.0
35	34.58275843852071	36.0	36.0	36.0	32.0	36.0
36	34.48093146622315	36.0	36.0	36.0	32.0	36.0
37	34.357719682078766	36.0	36.0	36.0	32.0	36.0
38	34.275668969889296	36.0	36.0	36.0	32.0	36.0
39	34.05096904767543	36.0	36.0	36.0	32.0	36.0
40	34.17368533230347	36.0	36.0	36.0	32.0	36.0
41	34.139534121153275	36.0	36.0	36.0	32.0	36.0
42	34.117604624176366	36.0	36.0	36.0	32.0	36.0
43	34.11186448088228	36.0	36.0	36.0	32.0	36.0
44	33.925815342632454	36.0	36.0	36.0	32.0	36.0
45	33.86818073344858	36.0	36.0	36.0	32.0	36.0
46	33.59108538076041	36.0	36.0	36.0	21.0	36.0
47	33.623577032868745	36.0	36.0	36.0	21.0	36.0
48	33.503416578466535	36.0	36.0	36.0	21.0	36.0
49	33.42006518004667	36.0	36.0	36.0	21.0	36.0
50	32.97426863724881	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	0.0
20	3.0
21	8.0
22	41.0
23	125.0
24	345.0
25	920.0
26	2029.0
27	4011.0
28	6951.0
29	10718.0
30	15491.0
31	21929.0
32	31737.0
33	49971.0
34	98537.0
35	306123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.831403970952074	21.73128846427127	12.94622198416429	25.491085580612367
2	10.224852760881484	21.60029292978976	49.88404862488774	18.29080568444102
3	18.73836389537616	27.85123274395287	31.60192954395578	21.808473816715185
4	9.295534492777913	18.7457304154727	42.12893553223388	29.8297995595155
5	8.534432662162237	43.26694489936077	37.61187450017397	10.586747938303024
6	27.684861159436657	41.93179934382509	19.227090806082277	11.156248690655975
7	24.742549745783244	33.28135446250143	23.356244113666133	18.61985167804919
8	20.961451230642272	46.18037275408468	19.718148216292825	13.140027798980219
9	26.309442266493726	14.131337715143477	21.979247125347946	37.579972893014855
10	15.716614899760446	29.782943335731915	36.08860794083088	18.41183382367676
11	32.408400902829264	20.02018431853332	31.8207239029331	15.75069087570431
12	26.394822030054232	24.213166806633136	35.59216746426301	13.799843699049624
13	30.649671457120007	23.695529011420213	27.72184158895615	17.93295794250363
14	17.1264307093112	27.84853016990897	28.279906219429773	26.745132901350054
15	18.594614486343108	42.69552591739735	22.95756917712514	15.752290419134404
16	16.332200228078424	27.751970182207696	37.664455107535694	18.251374482178186
17	15.851976806250581	33.00391116713818	32.71043700506976	18.433675021541475
18	16.19508942722129	27.308184166517897	41.57354746802007	14.92317893824075
19	24.362909675174564	24.50390843460408	29.208421305750527	21.924760584470825
20	23.139869566801472	30.86038547017889	31.327831821328378	14.67191314169126
21	20.32768548896876	26.058173829245767	28.376455757540427	25.237684924245045
22	22.071486995822852	34.01744091784333	28.602995961299886	15.308076125033928
23	17.809511838334817	33.5818135778787	32.080730575639826	16.527944008146655
24	23.935446379288045	28.267621721174844	32.48430153441457	15.312630365122537
25	20.144972683269845	31.245639251563496	33.38853152764191	15.220856537524751
26	13.800698478977313	33.661075555604135	36.36426571349974	16.17396025191881
27	16.25612802028737	33.044216478749824	33.191416490409225	17.508239010553588
28	15.222282340381732	33.59639582524927	30.59868578059787	20.58263605377114
29	19.12361048869263	28.232902316125436	29.149223768249005	23.49426342693293
30	15.783902936675654	30.364716169933686	32.415470378197185	21.43591051519347
31	21.922060886516423	27.008690265809175	31.662081655705148	19.407167191969247
32	17.11484405030177	33.44907428492313	31.61276453881846	17.82331712595664
33	15.834096949845424	33.54168070931624	35.13261422822509	15.491608112613244
34	18.23678472079267	32.180664530385535	34.7101355548714	14.872415193950403
35	16.552048283639053	34.4073758978946	32.747944611539324	16.29263120692702
36	14.753190442790649	32.11812608739217	34.843552162942586	18.285131306874597
37	16.548008992235637	32.149610688663884	28.821791232934608	22.480589086165875
38	18.435258515931448	36.09421315653791	28.90798221114518	16.56254611638546
39	21.426086006030076	29.465399925307196	29.730650465008246	19.377863603654482
40	23.53663446421447	29.91366730852961	31.89882734008231	14.650870887173612
41	21.978834764327708	26.910163430305744	34.92136362559868	16.189638179767872
42	18.91557090652064	35.12061559313142	31.599427253549607	14.364386246798341
43	14.674570441727269	35.887243685967036	33.40717314951105	16.031012722794642
44	14.664759944255696	34.684980917594984	31.2916829861459	19.358576152003426
45	15.29889695501289	34.893020120779326	31.284760490768488	18.523322433439297
46	18.51279024436453	28.94938189290516	30.48107787092802	22.056749991802292
47	22.79046211973541	30.276885633244493	29.34579643559162	17.586855811428478
48	16.471925586682552	32.07489424958192	28.82987754447711	22.623302619258418
49	20.598136765900705	30.03854715833118	29.835427680357345	19.527888395410777
50	16.55769808811608	36.280069589295636	27.604179001156787	19.558053321431498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	46.5
2	36.0
3	75.0
4	114.0
5	145.0
6	176.0
7	182.0
8	188.0
9	245.0
10	302.0
11	481.5
12	661.0
13	1234.5
14	1808.0
15	2489.0
16	3170.0
17	3815.0
18	4460.0
19	4662.0
20	4864.0
21	5222.5
22	5581.0
23	5822.5
24	6064.0
25	7493.0
26	8922.0
27	13316.0
28	17710.0
29	23201.0
30	28692.0
31	33851.0
32	39010.0
33	50584.5
34	62159.0
35	73454.0
36	84749.0
37	82213.0
38	79677.0
39	67783.5
40	55890.0
41	47139.5
42	38389.0
43	29911.5
44	21434.0
45	18785.0
46	16136.0
47	14790.0
48	13444.0
49	11521.5
50	9599.0
51	9244.5
52	8890.0
53	7978.5
54	7067.0
55	6641.5
56	6216.0
57	6086.0
58	5956.0
59	5218.0
60	4480.0
61	4005.5
62	3531.0
63	3069.0
64	2607.0
65	2372.5
66	2138.0
67	1899.0
68	1660.0
69	1439.0
70	1218.0
71	944.0
72	670.0
73	613.0
74	556.0
75	418.5
76	281.0
77	216.0
78	151.0
79	124.5
80	98.0
81	74.0
82	50.0
83	43.5
84	37.0
85	29.5
86	22.0
87	14.0
88	6.0
89	5.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010019291690728148
2	7.286757593256835E-4
3	5.465068194942626E-4
4	0.0
5	0.0
6	3.6433787966284176E-4
7	0.0
8	0.0
9	9.108446991571045E-4
10	0.0010930136389885252
11	0.0
12	0.0
13	3.6433787966284176E-4
14	0.0
15	0.004372054555954101
16	0.001275182578819946
17	0.0
18	5.465068194942626E-4
19	0.0
20	1.8216893983142088E-4
21	0.0
22	3.6433787966284176E-4
23	7.286757593256835E-4
24	3.6433787966284176E-4
25	0.001821689398314209
26	0.006193743954268309
27	0.005100730315279784
28	0.004007716676291259
29	0.001275182578819946
30	0.0038255477364598383
31	0.009290615931402464
32	0.002550365157639892
33	0.0032790409169655755
34	0.0021860272779770503
35	0.0032790409169655755
36	0.006558081833931151
37	0.00418988561612268
38	0.01056579851022241
39	0.0047363924356169426
40	0.0032790409169655755
41	0.004007716676291259
42	0.001275182578819946
43	9.108446991571045E-4
44	0.0010930136389885252
45	0.0010930136389885252
46	0.001275182578819946
47	0.002550365157639892
48	0.001275182578819946
49	5.465068194942626E-4
50	0.0010930136389885252
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	548941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.61586250002649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63412684354803	29.17079444343508
2	13.070557872264468	10.356028469325357
3	4.748218206850339	5.64314278808116
4	2.3432525239491837	3.7131987916644347
5	1.350851174903588	2.6757567201489887
6	0.9133741372880047	2.1710462540329147
7	0.5744391370375809	1.5929831307260274
8	0.4465847969173412	1.4153473527423712
9	0.33996620967110475	1.2121249155287095
>10	2.175238146745555	16.50313533494074
>50	0.22589893246290751	6.202513011192407
>100	0.15997332564049335	12.645375456131152
>500	0.013369528655833738	3.6594845029391365
>1k	0.004149164065603574	3.039068829111505
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2982	0.543227778577297	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	2467	0.44941077456411527	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	2301	0.4191707305520994	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2053	0.37399283347390705	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1654	0.3013074264811701	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1470	0.26778834155218867	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1441	0.26250544229707745	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	1173	0.21368416642225668	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1099	0.20020366487473154	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	982	0.17888989891445528	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	974	0.1774325473958039	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	899	0.16376987690844735	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	875	0.15939782235249325	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	869	0.15830480871350472	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	854	0.1555722746160334	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	804	0.1464638276244624	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	737	0.13425850865575717	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	730	0.13298332607693725	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	726	0.13225465031761155	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	723	0.1317081434981173	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	719	0.1309794677387916	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC	713	0.12988645409980307	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	680	0.1238748790853662	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	677	0.12332837226587193	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	673	0.12259969650654624	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	668	0.12168885180738914	No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTCTCATTGTAACTCAT	608	0.11075871541750387	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	605	0.11021220859800962	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	601	0.10948353283868394	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	592	0.10784401238020115	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	585	0.10656882980138119	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG	572	0.10420063358357273	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATG	561	0.10219677524542711	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	1.8216893983142085E-4	0.0
18	0.0	0.0	0.0	1.8216893983142085E-4	0.0
19	0.0	0.0	0.0	1.8216893983142085E-4	0.0
20	0.0	0.0	0.0	1.8216893983142085E-4	0.0
21	0.0	0.0	0.0	5.465068194942626E-4	0.0
22	0.0	0.0	0.0	9.108446991571043E-4	0.0
23	0.0	0.0	0.0	0.0020038583381456294	0.0
24	0.0	0.0	0.0	0.0032790409169655755	0.0
25	0.0	0.0	0.0	0.0036433787966284174	0.0
26	0.0	0.0	0.0	0.004372054555954101	0.0
27	0.0	0.0	0.0	0.005829406074605467	0.0
28	0.0	0.0	0.0	0.007651095472919677	0.0
29	0.0	0.0	0.0	0.011112305329716674	0.0
30	0.0	0.0	0.0	0.017488218223816403	0.0
31	0.0	0.0	0.0	0.034612098567969964	0.0
32	0.0	0.0	0.0	0.05483285088925768	0.0
33	0.0	0.0	0.0	0.06904202819610851	0.0
34	0.0	0.0	0.0	0.09217748355469896	0.0
35	0.0	0.0	0.0	0.1134912495149752	0.0
36	0.0	0.0	0.0	0.14682816550412522	0.0
37	0.0	0.0	0.0	0.20238969215270858	0.0
38	0.0	0.0	0.0	0.27015653776999715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGGA	20	7.846637E-4	44.007107	9
TCTAGCG	20	7.8501593E-4	44.003098	28
CTGTATG	25	4.4427215E-5	43.995083	1
GTGCACT	30	1.299671E-4	36.669247	11
TATACTC	30	1.3003679E-4	36.66591	5
TAGCGGC	25	0.0023498142	35.205685	30
CTGTTCA	25	0.0023498142	35.205685	9
CGAGTAA	25	0.002350865	35.20248	21
TGTGCAC	35	3.2125463E-4	31.433647	10
TAGGAAC	70	1.033186E-9	31.427921	4
CTAGGTA	35	3.2159817E-4	31.427921	4
GTATAGT	35	3.2177003E-4	31.425058	1
TAGGACC	950	0.0	30.104641	4
CCGGTCC	30	0.0057377852	29.33807	9
TGGCTAA	30	0.0057403413	29.335398	26
CTAGCGG	30	0.0057403413	29.335398	29
AGAACGT	60	2.9409603E-7	29.332727	5
GTGGTAC	30	0.005745455	29.330053	1
ACTAGAG	55	4.9588853E-6	27.99687	2
GGCGGGT	40	7.0205756E-4	27.504442	32
>>END_MODULE