FastQCFastQC Report
Thu 2 Feb 2017
SRR4063946_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063946_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772760
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17320.224131683834567No Hit
TATCAACGCAGAGTACTTTTTTTTT9710.12565350173404422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC402.7671937E-416.6184273
GGTATCA3400.016.1994761
TGGGCTA508.7198394E-515.1969435
TTATAAG400.00529432414.2425232
CGAACGA551.9539554E-413.82166916
CGGTCCA551.9571796E-413.81898310
CGATAAC551.9571796E-413.81898310
TTAATTC1001.009721E-813.2947443
GTCTAGA500.001502826913.2947441
CCGATAA604.0858847E-412.66749
TAGACTG1051.9930667E-812.664125
AATTCCG604.0948248E-412.6641195
GACGTGA1301.9645086E-1012.4221877
TAAATCT701.0915776E-412.2118294
TAAGACT701.0915776E-412.2118294
ACCGTCG550.003064711312.091618
TAGGTGA550.00307066412.0884774
GCACCGT550.00307066412.0884776
TAGGACG2200.012.0884774
ATAAGAC550.00307513512.086133
Produced by FastQC (version 0.11.5)