##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063946_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	772760
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315677571302864	32.0	32.0	32.0	32.0	32.0
2	31.02679615922149	32.0	32.0	32.0	32.0	32.0
3	31.01097624100626	32.0	32.0	32.0	32.0	32.0
4	31.03922045654537	32.0	32.0	32.0	32.0	32.0
5	31.008692220094208	32.0	32.0	32.0	32.0	32.0
6	34.645747709508775	36.0	36.0	36.0	32.0	36.0
7	34.615161240229824	36.0	36.0	36.0	32.0	36.0
8	34.596592732543094	36.0	36.0	36.0	32.0	36.0
9	34.67529892851597	36.0	36.0	36.0	32.0	36.0
10	34.45948289248926	36.0	36.0	36.0	32.0	36.0
11	34.62546845074797	36.0	36.0	36.0	32.0	36.0
12	34.51069025311869	36.0	36.0	36.0	32.0	36.0
13	34.536726797453284	36.0	36.0	36.0	32.0	36.0
14	34.48497463636834	36.0	36.0	36.0	32.0	36.0
15	34.45196309332781	36.0	36.0	36.0	32.0	36.0
16	34.44182411097883	36.0	36.0	36.0	32.0	36.0
17	34.404883793157	36.0	36.0	36.0	32.0	36.0
18	34.38935892126921	36.0	36.0	36.0	32.0	36.0
19	34.38724183446348	36.0	36.0	36.0	32.0	36.0
20	34.38041824110979	36.0	36.0	36.0	32.0	36.0
21	34.35804389461152	36.0	36.0	36.0	32.0	36.0
22	34.337628759252546	36.0	36.0	36.0	32.0	36.0
23	34.28567472436462	36.0	36.0	36.0	32.0	36.0
24	34.27962239246338	36.0	36.0	36.0	32.0	36.0
25	33.90171722138827	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	7.0
5	11.0
6	23.0
7	9.0
8	42.0
9	45.0
10	72.0
11	24.0
12	52.0
13	38.0
14	71.0
15	110.0
16	189.0
17	276.0
18	287.0
19	370.0
20	584.0
21	811.0
22	1310.0
23	2134.0
24	3137.0
25	4872.0
26	7227.0
27	9697.0
28	13533.0
29	18534.0
30	24541.0
31	33030.0
32	46511.0
33	66602.0
34	150907.0
35	387700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04797659425731	17.775677704994433	12.117391191776921	26.05895450897134
2	15.770402445368733	19.829409130791507	38.76781679405119	25.632371629788565
3	19.381142057027795	23.291611991521147	28.75343256386963	28.57381338758143
4	12.88721631313517	16.007205649601993	35.9379185663242	35.16765947093864
5	14.548687592370669	36.174230168939644	34.15870542589511	15.118376812794585
6	33.60003002602687	35.64917693742291	17.10694054199567	13.643852494554547
7	29.414094555658814	30.721916896847194	20.84283610024567	19.02115244724832
8	26.824563810865182	34.57085306400375	19.520483219113355	19.084099906017716
9	26.843434016494257	15.074638459845415	19.02380921555173	39.0581183081086
10	15.946326348542076	27.357091895993378	32.25546648504397	24.44111527042058
11	35.81397697565624	21.896433181883513	22.987571101353986	19.30201874110626
12	24.036940065624037	23.937752985323936	29.659785724829657	22.365521224222366
13	29.279198202689493	20.63372029031485	25.141369866561348	24.945711640434308
14	23.04769748343136	19.959805345992578	25.781944002009734	31.210553168566328
15	24.443926504292428	27.72417097204418	22.840124823576634	24.991777700086754
16	24.653119047341562	26.801676682995808	24.352952907066335	24.192251362596295
17	23.30062892430248	26.353443286100347	26.05730371851332	24.288624071083856
18	23.377351624429053	26.48336719853816	26.88133191915346	23.257949257879325
19	24.963545619128773	25.472352945747073	25.849846800950015	23.714254634174136
20	25.201804291603153	25.275493777835912	25.697298095340464	23.825403835220467
21	25.377548826885672	25.350095439370747	25.362397664908098	23.909958068835486
22	25.114416624146248	25.235891206431678	25.692910183921324	23.956781985500754
23	24.25900551455828	25.613815147033304	25.98563987266525	24.14153946574317
24	24.39698769045476	25.4357553113648	25.781147811672376	24.386109186508065
25	24.195516024927088	25.26095222307983	25.95626363038287	24.58726812161021
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	29.0
2	92.0
3	155.0
4	155.0
5	155.0
6	369.0
7	583.0
8	583.0
9	583.0
10	828.5
11	1074.0
12	1074.0
13	1074.0
14	1575.5
15	2077.0
16	2077.0
17	2077.0
18	3977.5
19	5878.0
20	5878.0
21	5878.0
22	9060.5
23	12243.0
24	12243.0
25	12243.0
26	18039.0
27	23835.0
28	23835.0
29	23835.0
30	32144.5
31	40454.0
32	40454.0
33	40454.0
34	47173.5
35	53893.0
36	53893.0
37	53893.0
38	63169.5
39	72446.0
40	72446.0
41	72446.0
42	80141.5
43	87837.0
44	87837.0
45	87837.0
46	94030.0
47	100223.0
48	100223.0
49	100223.0
50	101375.5
51	102528.0
52	102528.0
53	102528.0
54	95532.0
55	88536.0
56	88536.0
57	88536.0
58	79778.0
59	71020.0
60	71020.0
61	71020.0
62	60401.0
63	49782.0
64	49782.0
65	49782.0
66	40204.0
67	30626.0
68	30626.0
69	30626.0
70	23008.0
71	15390.0
72	15390.0
73	15390.0
74	11607.0
75	7824.0
76	7824.0
77	7824.0
78	5760.0
79	3696.0
80	3696.0
81	3696.0
82	2589.5
83	1483.0
84	1483.0
85	1483.0
86	983.0
87	483.0
88	483.0
89	483.0
90	336.5
91	190.0
92	190.0
93	190.0
94	138.5
95	87.0
96	87.0
97	87.0
98	237.5
99	388.0
100	388.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038821885190744865
2	0.0043998136549510845
3	0.0018116879755680937
4	0.004270407370981935
5	0.007505564470210673
6	0.012552409545007505
7	0.023034318546508618
8	0.035457321807546974
9	0.047880325068585326
10	0.059915109477716236
11	0.05784460893420984
12	0.06237382887313008
13	0.06405611056472903
14	0.06703245509601946
15	0.06082095346550029
16	0.06742067394792692
17	0.06327967286091413
18	0.07518505098607589
19	0.07272633159066204
20	0.07777317666545887
21	0.07013820591127905
22	0.07531445727004503
23	0.08049070862881101
24	0.07699673896164398
25	0.07609089497385993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	772760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.86651867889417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.92929708043187	23.094394041122467
2	16.9285033079707	13.497609866658713
3	8.589987888792127	10.273587378600178
4	4.954365418486292	7.900532059926042
5	3.1647719945716357	6.308422091801565
6	2.096888768201595	5.015739314704336
7	1.4617753214509026	4.079312520987825
8	0.9851895269712899	3.142086134340147
9	0.7552561476540363	2.709848998601936
>10	3.015121412015034	18.745221246594195
>50	0.08194964236141696	2.2461901853394233
>100	0.03591401787835149	2.547764055164411
>500	6.52982143213665E-4	0.21385390530931891
>1k	3.264910716068325E-4	0.22543820084950725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1732	0.224131683834567	No Hit
TATCAACGCAGAGTACTTTTTTTTT	971	0.12565350173404422	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5881256793829907E-4
2	0.0	0.0	0.0	0.0	3.882188519074486E-4
3	0.0	0.0	0.0	0.0	3.882188519074486E-4
4	0.0	0.0	0.0	0.0	5.176251358765981E-4
5	0.0	0.0	0.0	0.0	5.176251358765981E-4
6	0.0	0.0	0.0	0.0	7.764377038148973E-4
7	0.0	0.0	0.0	0.0	7.764377038148973E-4
8	0.0	0.0	0.0	0.0	9.058439877840468E-4
9	0.0	0.0	0.0	0.0	9.058439877840468E-4
10	0.0	0.0	0.0	0.0	9.058439877840468E-4
11	0.0	0.0	0.0	0.0	9.058439877840468E-4
12	0.0	0.0	0.0	0.0	9.058439877840468E-4
13	0.0	0.0	0.0	1.2940628396914953E-4	9.058439877840468E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGAC	40	2.7671937E-4	16.618427	3
GGTATCA	340	0.0	16.199476	1
TGGGCTA	50	8.7198394E-5	15.196943	5
TTATAAG	40	0.005294324	14.242523	2
CGAACGA	55	1.9539554E-4	13.821669	16
CGGTCCA	55	1.9571796E-4	13.818983	10
CGATAAC	55	1.9571796E-4	13.818983	10
TTAATTC	100	1.009721E-8	13.294744	3
GTCTAGA	50	0.0015028269	13.294744	1
CCGATAA	60	4.0858847E-4	12.6674	9
TAGACTG	105	1.9930667E-8	12.66412	5
AATTCCG	60	4.0948248E-4	12.664119	5
GACGTGA	130	1.9645086E-10	12.422187	7
TAAATCT	70	1.0915776E-4	12.211829	4
TAAGACT	70	1.0915776E-4	12.211829	4
ACCGTCG	55	0.0030647113	12.09161	8
TAGGTGA	55	0.003070664	12.088477	4
GCACCGT	55	0.003070664	12.088477	6
TAGGACG	220	0.0	12.088477	4
ATAAGAC	55	0.003075135	12.08613	3
>>END_MODULE