FastQCFastQC Report
Thu 2 Feb 2017
SRR4063946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772760
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.028.986531
GTATCAA10650.026.439761
CCCTATA508.348288E-526.3950312
GATATAC2200.021.9987071
TATCAAC14400.019.2463782
TAGGACG3100.019.1601644
GTATTAC708.122913E-418.8560331
ATCAACG14850.018.664363
GTACTAG951.5967033E-518.5252271
CCTATAC600.007415023618.331073
TCAACGC15250.018.3202344
CAACGCA15150.018.2959545
TTAGGAC2550.018.1154083
ATACACT3700.017.8367884
AACGCAG15600.017.7681856
GACGTGA2250.017.5989657
TAAACTA750.001290807817.5989655
GTAGAAC3900.017.4850183
GTCCTAC2400.017.4156421
GTCCTAA2400.017.4156421
Produced by FastQC (version 0.11.5)