##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	772760
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37941016615767	32.0	32.0	32.0	32.0	32.0
2	31.47262151250065	32.0	32.0	32.0	32.0	32.0
3	31.50872845385372	32.0	32.0	32.0	32.0	32.0
4	31.57365417464672	32.0	32.0	32.0	32.0	32.0
5	31.548225839846783	32.0	32.0	32.0	32.0	32.0
6	35.13122314819608	36.0	36.0	36.0	36.0	36.0
7	35.14011465396759	36.0	36.0	36.0	36.0	36.0
8	35.09752963403903	36.0	36.0	36.0	36.0	36.0
9	35.18106009627827	36.0	36.0	36.0	36.0	36.0
10	35.06766395776179	36.0	36.0	36.0	36.0	36.0
11	35.18287825456804	36.0	36.0	36.0	36.0	36.0
12	35.111808323412184	36.0	36.0	36.0	36.0	36.0
13	35.13090998498887	36.0	36.0	36.0	36.0	36.0
14	35.08229722035302	36.0	36.0	36.0	36.0	36.0
15	35.066425539624205	36.0	36.0	36.0	36.0	36.0
16	35.07844997153062	36.0	36.0	36.0	36.0	36.0
17	35.06112894042135	36.0	36.0	36.0	36.0	36.0
18	35.04693954138413	36.0	36.0	36.0	36.0	36.0
19	35.049488845178324	36.0	36.0	36.0	36.0	36.0
20	35.03053858895388	36.0	36.0	36.0	36.0	36.0
21	35.019650344220715	36.0	36.0	36.0	36.0	36.0
22	35.013539779491694	36.0	36.0	36.0	36.0	36.0
23	34.97127439308453	36.0	36.0	36.0	36.0	36.0
24	34.949556136445985	36.0	36.0	36.0	36.0	36.0
25	34.92153967596666	36.0	36.0	36.0	36.0	36.0
26	34.86254982141933	36.0	36.0	36.0	32.0	36.0
27	34.86492442673016	36.0	36.0	36.0	32.0	36.0
28	34.835182980485534	36.0	36.0	36.0	32.0	36.0
29	34.82005797401522	36.0	36.0	36.0	32.0	36.0
30	34.808292354676745	36.0	36.0	36.0	32.0	36.0
31	34.800952430250014	36.0	36.0	36.0	32.0	36.0
32	34.769649050157874	36.0	36.0	36.0	32.0	36.0
33	34.753272684921576	36.0	36.0	36.0	32.0	36.0
34	34.73671903307625	36.0	36.0	36.0	32.0	36.0
35	34.7109154200528	36.0	36.0	36.0	32.0	36.0
36	34.697894559759824	36.0	36.0	36.0	32.0	36.0
37	34.67402168849319	36.0	36.0	36.0	32.0	36.0
38	34.65047492106217	36.0	36.0	36.0	32.0	36.0
39	34.637443708266474	36.0	36.0	36.0	32.0	36.0
40	34.62272633159066	36.0	36.0	36.0	32.0	36.0
41	34.60129794502821	36.0	36.0	36.0	32.0	36.0
42	34.56050779025829	36.0	36.0	36.0	32.0	36.0
43	34.554855323774525	36.0	36.0	36.0	32.0	36.0
44	34.51304415342409	36.0	36.0	36.0	32.0	36.0
45	34.47117086805735	36.0	36.0	36.0	32.0	36.0
46	34.44566877167555	36.0	36.0	36.0	32.0	36.0
47	34.42852632123816	36.0	36.0	36.0	32.0	36.0
48	34.38693643563332	36.0	36.0	36.0	32.0	36.0
49	34.3587439826078	36.0	36.0	36.0	32.0	36.0
50	33.78561002122263	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	4.0
20	3.0
21	22.0
22	78.0
23	187.0
24	574.0
25	1253.0
26	2682.0
27	5001.0
28	8271.0
29	13051.0
30	18964.0
31	26663.0
32	37678.0
33	58528.0
34	121626.0
35	478172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4409763698413	18.67104784657415	12.689546641059454	26.198429142525097
2	15.401498014897328	20.6396326913023	38.15848728447272	25.800382009327656
3	18.61290915349516	23.96144180424818	29.10874612101652	28.316902921240132
4	12.714685025104819	16.752419897510222	35.818494746104875	34.71440033128009
5	14.300300222578807	36.69172835032869	33.93369222009421	15.074279206998293
6	33.85462498058906	35.79209586417517	16.85141570474662	13.501863450489154
7	29.52447011293301	30.936553310928762	20.776593996317093	18.762382579821136
8	27.08784098555826	34.6245923702055	19.352709767586315	18.93485687664993
9	27.12057860961321	14.944956253582989	18.99025427402688	38.94421086277692
10	15.92735517233347	27.723081064153032	32.16697336511609	24.1825903983974
11	36.33379056887002	21.87017961592215	22.77667063512604	19.019359180081786
12	24.187975568093588	24.183834567006574	29.49272736684093	22.135462498058907
13	29.475045033853036	20.789075926040955	25.115431600306437	24.620447439799573
14	23.31237374750407	19.832366448961317	25.77162031531258	31.083639488222044
15	24.619171423653754	27.87298941535324	22.72910214915306	24.77873701183995
16	24.6751246832773	26.86080247632506	24.371277942124184	24.092794898273457
17	23.591542005279777	26.467855479062063	25.908949738599308	24.031652777058852
18	23.69567664867481	26.61858773197784	26.661291971473567	23.02444364787378
19	25.27084735234743	25.654795796883896	25.559811584450543	23.51454526631813
20	25.450529143664642	25.38453176803087	25.544607755597482	23.620331332707007
21	25.755473885811895	25.42561726797453	25.152311196231693	23.66659764998188
22	25.32380729308879	25.39407499621486	25.57175005402719	23.710367656669156
23	24.524168167795104	25.560982048574445	25.773469366627328	24.141380417003127
24	24.585575303005598	25.532055313565177	25.674273187725007	24.20809619570422
25	24.559945958956757	25.210353725545005	25.87797283971706	24.351727475781175
26	24.163969515832846	26.065153501421584	26.11911808667722	23.65175889606835
27	24.497645303354034	25.63895169086502	25.973746976553976	23.88965602922697
28	24.166887742887276	25.841718053407703	25.888176412353523	24.103217791351497
29	24.182648756373425	25.755105210031836	25.513238605481792	24.549007428112947
30	24.33935698275728	25.800277457126146	25.7157720917472	24.14459346836937
31	24.499837582292717	25.584477267344724	25.517957186433527	24.397727963929032
32	24.24269090607918	26.03605888686434	25.693250993871132	24.027999213185357
33	24.0921367479336	25.767644466760963	25.98001089655904	24.160207888746392
34	24.721378175098224	25.529032057686234	26.054958252020104	23.694631515195436
35	24.654345080337915	25.431971591850257	26.043823505052178	23.869859822759647
36	24.260714942034873	25.640411747805764	25.869735708018315	24.229137602141044
37	24.67271795508898	25.516999513412227	25.395352569079936	24.414929962418856
38	24.38446117947915	25.857224759511166	25.522941075220945	24.235372985788736
39	24.53062154145594	25.397295800311625	25.646673327087033	24.425409331145403
40	25.266390419521233	25.39851799704428	25.549410019955015	23.78568156347947
41	24.355435462017184	24.99912647480171	26.545719014767105	24.099719048414002
42	24.693462121300357	25.51689242089543	25.969047924083426	23.820597533720782
43	23.982327911960493	24.828921350408415	26.536990258093233	24.651760479537856
44	23.836526901938658	25.280588443107803	26.157843859147384	24.72504079580615
45	24.168520438072388	25.348851955809714	25.83633236665463	24.646295239463267
46	24.219930844565916	24.965318756858604	25.97935689587345	24.83539350270203
47	23.999534125320288	25.384346610761703	26.082123353262414	24.53399591065559
48	23.948459198159295	25.23943800493564	26.03439418076033	24.777708616144743
49	23.87995191243819	25.045518837098857	26.148970504311187	24.925558746151765
50	23.914596365725703	25.740662617081245	25.875763826521762	24.468977190671286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	7.0
2	12.0
3	35.0
4	58.0
5	80.5
6	103.0
7	96.5
8	90.0
9	149.0
10	208.0
11	296.0
12	384.0
13	633.5
14	883.0
15	1206.0
16	1529.0
17	1554.0
18	1579.0
19	1582.5
20	1586.0
21	1918.0
22	2250.0
23	2792.0
24	3334.0
25	4264.0
26	5194.0
27	6811.0
28	8428.0
29	10194.0
30	11960.0
31	13917.0
32	15874.0
33	18576.0
34	21278.0
35	25186.5
36	29095.0
37	31728.5
38	34362.0
39	36856.5
40	39351.0
41	42080.0
42	44809.0
43	46516.0
44	48223.0
45	49638.5
46	51054.0
47	52554.0
48	54054.0
49	55745.0
50	57436.0
51	56837.0
52	56238.0
53	56301.0
54	56364.0
55	55248.5
56	54133.0
57	50059.5
58	45986.0
59	42589.5
60	39193.0
61	33731.5
62	28270.0
63	24143.0
64	20016.0
65	16715.5
66	13415.0
67	11619.0
68	9823.0
69	7971.0
70	6119.0
71	4845.5
72	3572.0
73	3021.0
74	2470.0
75	1985.0
76	1500.0
77	1231.0
78	962.0
79	841.0
80	720.0
81	547.0
82	374.0
83	296.0
84	218.0
85	173.0
86	128.0
87	92.5
88	57.0
89	47.0
90	37.0
91	24.5
92	12.0
93	10.5
94	9.0
95	6.5
96	4.0
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007893783322118122
2	5.176251358765982E-4
3	7.764377038148974E-4
4	0.0
5	0.0
6	0.0
7	1.2940628396914956E-4
8	0.0
9	0.001423469123660645
10	0.0018116879755680937
11	0.0
12	0.0
13	0.0010352502717531965
14	3.882188519074487E-4
15	0.004788032506858534
16	2.588125679382991E-4
17	0.0
18	3.882188519074487E-4
19	0.0
20	2.588125679382991E-4
21	0.0
22	1.2940628396914956E-4
23	0.0010352502717531965
24	2.588125679382991E-4
25	0.0018116879755680937
26	0.004011594803043636
27	0.006340907914488328
28	0.0032351570992287383
29	0.002588125679382991
30	0.0036233759511361875
31	0.007893783322118122
32	0.0031057508152595894
33	0.005046845074796832
34	0.0036233759511361875
35	0.0031057508152595894
36	0.0069879393343340755
37	0.004141001087012786
38	0.008152595890056421
39	0.004658626222889384
40	0.00284693824732129
41	0.0037527822351053363
42	0.0021999068274755423
43	0.002070500543506393
44	0.001164656555722346
45	0.001423469123660645
46	0.0010352502717531965
47	0.002588125679382991
48	0.001682281691598944
49	3.882188519074487E-4
50	7.764377038148974E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	772760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.12215619090637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.70520852213763	27.225668482062034
2	15.837801760264858	13.975959259740357
3	7.926768644575832	10.49238372675462
4	4.507402539512586	7.955052754546492
5	2.8045108685994466	6.187053329171962
6	1.8383257068121162	4.866653637943353
7	1.2165561639710296	3.757395675721776
8	0.9147801141988229	3.22896568632125
9	0.6849346913855381	2.7198715890494665
>10	2.499180963676523	16.80737698457035
>50	0.04626140398758247	1.4106020973102467
>100	0.018268620877989618	1.373016776808118
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.882188519074486E-4
7	0.0	0.0	0.0	0.0	3.882188519074486E-4
8	0.0	0.0	0.0	0.0	3.882188519074486E-4
9	0.0	0.0	0.0	0.0	3.882188519074486E-4
10	0.0	0.0	0.0	0.0	3.882188519074486E-4
11	0.0	0.0	0.0	0.0	3.882188519074486E-4
12	0.0	0.0	0.0	0.0	3.882188519074486E-4
13	0.0	0.0	0.0	1.2940628396914953E-4	3.882188519074486E-4
14	0.0	0.0	0.0	1.2940628396914953E-4	7.764377038148973E-4
15	0.0	0.0	0.0	1.2940628396914953E-4	9.058439877840468E-4
16	0.0	0.0	0.0	1.2940628396914953E-4	0.0010352502717531963
17	0.0	0.0	0.0	1.2940628396914953E-4	0.0010352502717531963
18	0.0	0.0	0.0	1.2940628396914953E-4	0.0010352502717531963
19	0.0	0.0	0.0	1.2940628396914953E-4	0.0010352502717531963
20	0.0	0.0	0.0	1.2940628396914953E-4	0.0010352502717531963
21	0.0	0.0	0.0	1.2940628396914953E-4	0.0010352502717531963
22	0.0	0.0	0.0	2.5881256793829907E-4	0.0011646565557223458
23	0.0	0.0	0.0	2.5881256793829907E-4	0.0011646565557223458
24	0.0	0.0	0.0	5.176251358765981E-4	0.001423469123660645
25	0.0	0.0	0.0	6.470314198457477E-4	0.001423469123660645
26	0.0	0.0	0.0	6.470314198457477E-4	0.001423469123660645
27	0.0	0.0	0.0	0.0011646565557223458	0.001423469123660645
28	0.0	0.0	0.0	0.0054350639267042805	0.001423469123660645
29	0.0	0.0	0.0	0.015269941508359646	0.0015528754076297945
30	0.0	0.0	0.0	0.030022257880842694	0.0015528754076297945
31	0.0	0.0	0.0	0.06586779854029712	0.001682281691598944
32	0.0	0.0	0.0	0.10740721569439413	0.0021999068274755423
33	0.0	0.0	0.0	0.14804078886070707	0.0021999068274755423
34	0.0	0.0	0.0	0.19605052021326155	0.0021999068274755423
35	0.0	0.0	0.0	0.2558362234070086	0.0021999068274755423
36	0.0	0.0	0.0	0.33580930689994304	0.0021999068274755423
37	0.0	0.0	0.0	0.4649567783011543	0.0021999068274755423
38	0.0	0.0	0.0	0.6527252963403903	0.0021999068274755423
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	425	0.0	28.98653	1
GTATCAA	1065	0.0	26.43976	1
CCCTATA	50	8.348288E-5	26.395031	2
GATATAC	220	0.0	21.998707	1
TATCAAC	1440	0.0	19.246378	2
TAGGACG	310	0.0	19.160164	4
GTATTAC	70	8.122913E-4	18.856033	1
ATCAACG	1485	0.0	18.66436	3
GTACTAG	95	1.5967033E-5	18.525227	1
CCTATAC	60	0.0074150236	18.33107	3
TCAACGC	1525	0.0	18.320234	4
CAACGCA	1515	0.0	18.295954	5
TTAGGAC	255	0.0	18.115408	3
ATACACT	370	0.0	17.836788	4
AACGCAG	1560	0.0	17.768185	6
GACGTGA	225	0.0	17.598965	7
TAAACTA	75	0.0012908078	17.598965	5
GTAGAAC	390	0.0	17.485018	3
GTCCTAC	240	0.0	17.415642	1
GTCCTAA	240	0.0	17.415642	1
>>END_MODULE