FastQCFastQC Report
Thu 2 Feb 2017
SRR4063945_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063945_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299551
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30220.23254185484063344No Hit
TATCAACGCAGAGTACTTTTTTTTT19880.1529759124497615No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13560.10434373102710089No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13350.10272778828995553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG954.8985385E-913.9999717
CGAACGA1301.8189894E-1213.88833116
ACGAACG1351.8189894E-1213.37394915
CGCGGTC1051.9934305E-812.66615410
ACTAGAC604.1046028E-412.6617643
CGTTATT751.4863857E-512.6598152
AACGAGA1457.2759576E-1212.45208718
CCGACCA2150.012.3725449
GCGTAAC701.09104636E-412.21379111
GCGTTAT701.0945819E-412.20955851
ACCGTCT550.00306802412.0908848
CGACCAT2200.012.09041910
GCTCGAC550.003068909312.09041911
GATATAC550.003076885112.086231
GATAACG1501.4551915E-1112.032845511
GAACCGC1201.0017175E-811.8740626
TAAGACT1052.7265742E-711.7591634
TACACCG658.0297765E-411.6909335
TCGAACG658.0479536E-411.6877823
ACGAGAC1552.5465852E-1111.64827819
Produced by FastQC (version 0.11.5)