##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063945_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1299551
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2006100568581	32.0	32.0	32.0	32.0	32.0
2	30.83243443312344	32.0	32.0	32.0	32.0	32.0
3	30.84410615666488	32.0	32.0	32.0	32.0	32.0
4	30.82698870609926	32.0	32.0	32.0	32.0	32.0
5	30.801406793577165	32.0	32.0	32.0	32.0	32.0
6	34.238298458467575	36.0	36.0	36.0	32.0	36.0
7	34.21814380505267	36.0	36.0	36.0	32.0	36.0
8	34.18373422820651	36.0	36.0	36.0	32.0	36.0
9	34.355999110462	36.0	36.0	36.0	32.0	36.0
10	34.0226078083892	36.0	36.0	36.0	32.0	36.0
11	34.32843728333863	36.0	36.0	36.0	32.0	36.0
12	34.13141384986045	36.0	36.0	36.0	32.0	36.0
13	34.25201627331286	36.0	36.0	36.0	32.0	36.0
14	34.120601653955866	36.0	36.0	36.0	32.0	36.0
15	34.0443068413629	36.0	36.0	36.0	32.0	36.0
16	34.0468323290121	36.0	36.0	36.0	32.0	36.0
17	33.9743734566785	36.0	36.0	36.0	32.0	36.0
18	33.97076297890579	36.0	36.0	36.0	32.0	36.0
19	33.971971857972484	36.0	36.0	36.0	32.0	36.0
20	33.94808822431747	36.0	36.0	36.0	32.0	36.0
21	33.906551570503964	36.0	36.0	36.0	32.0	36.0
22	33.89462899109	36.0	36.0	36.0	32.0	36.0
23	33.85685209737825	36.0	36.0	36.0	32.0	36.0
24	33.82177921451332	36.0	36.0	36.0	32.0	36.0
25	33.17666563297631	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	10.0
5	19.0
6	57.0
7	15.0
8	78.0
9	74.0
10	112.0
11	41.0
12	74.0
13	64.0
14	176.0
15	407.0
16	713.0
17	964.0
18	1189.0
19	1425.0
20	1866.0
21	2339.0
22	3221.0
23	4490.0
24	6128.0
25	8657.0
26	12267.0
27	17051.0
28	24123.0
29	34298.0
30	48561.0
31	72027.0
32	111621.0
33	168526.0
34	342224.0
35	436731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44181617620456	17.120872370545023	11.269351179795564	26.16796027345485
2	16.743478792850432	19.59513378699814	37.402336761085685	26.259050659065743
3	18.834900952575893	23.664343004273086	28.662615455291878	28.83814058785914
4	12.494199687725809	15.22517524061196	36.14362150336399	36.13700356829824
5	14.504744387923935	36.44790408102014	33.91471645259845	15.132635078457476
6	34.26970875161231	35.56772040647328	16.779644320487126	13.382926521427274
7	30.238287653836387	30.489274746588467	20.8129949894941	18.459442610081044
8	28.166963922140365	32.90267853225331	19.3666110643696	19.563746481236727
9	27.19342613057667	14.105812260722223	18.483079938504698	40.217681670196406
10	15.35417975534942	26.74248863594368	32.10422903039038	25.799102578316518
11	37.17965793738476	21.08847203563056	22.505533592273373	19.22633643471131
12	24.491263569705495	23.548362010753358	29.010353745927837	22.95002067361331
13	29.2927737448836	19.46820207520381	25.342710453939283	25.896313725973307
14	23.261923791835873	19.51034337255699	25.45934183718255	31.768390998424582
15	24.922447464846314	27.183231867643677	22.38740927181076	25.506911395699248
16	25.5704383794114	25.753616202806107	23.82797762135242	24.847967796430073
17	23.87106460628739	25.96986425827116	25.274447755218322	24.884623380223132
18	24.6638698171267	25.117741821217244	26.159783891009113	24.05860447064694
19	25.304495608040206	24.98608141084826	25.593956059610832	24.115466921500698
20	25.562528252514138	24.38029289434349	25.09878272574866	24.958396127393716
21	26.42355619144719	24.352732990711637	24.678772907114816	24.544937910726357
22	25.541704592533726	24.36496312142592	25.093139768948685	25.00019251709167
23	24.167303929156432	24.331644491472236	25.63204502346127	25.86900655591006
24	24.504845277625474	24.95433487552596	25.47682706702069	25.063992779827878
25	24.483860087387626	24.677994920613408	25.47047471780758	25.36767027419139
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	88.0
2	256.5
3	425.0
4	425.0
5	425.0
6	1248.0
7	2071.0
8	2071.0
9	2071.0
10	2314.0
11	2557.0
12	2557.0
13	2557.0
14	3103.5
15	3650.0
16	3650.0
17	3650.0
18	6253.0
19	8856.0
20	8856.0
21	8856.0
22	14027.5
23	19199.0
24	19199.0
25	19199.0
26	28010.0
27	36821.0
28	36821.0
29	36821.0
30	45696.5
31	54572.0
32	54572.0
33	54572.0
34	68222.0
35	81872.0
36	81872.0
37	81872.0
38	94905.0
39	107938.0
40	107938.0
41	107938.0
42	123354.5
43	138771.0
44	138771.0
45	138771.0
46	154285.0
47	169799.0
48	169799.0
49	169799.0
50	173089.0
51	176379.0
52	176379.0
53	176379.0
54	164208.0
55	152037.0
56	152037.0
57	152037.0
58	139010.5
59	125984.0
60	125984.0
61	125984.0
62	110196.5
63	94409.0
64	94409.0
65	94409.0
66	77235.0
67	60061.0
68	60061.0
69	60061.0
70	45367.5
71	30674.0
72	30674.0
73	30674.0
74	23576.5
75	16479.0
76	16479.0
77	16479.0
78	13154.0
79	9829.0
80	9829.0
81	9829.0
82	6753.0
83	3677.0
84	3677.0
85	3677.0
86	2791.0
87	1905.0
88	1905.0
89	1905.0
90	1275.0
91	645.0
92	645.0
93	645.0
94	422.0
95	199.0
96	199.0
97	199.0
98	426.5
99	654.0
100	654.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03616633745039633
2	0.0046169792489867655
3	0.0016928923912951474
4	0.004001382015788531
5	0.00931090815212331
6	0.013773988092810517
7	0.021622852816088018
8	0.034165646442502064
9	0.04540029594836986
10	0.0553268013336914
11	0.0512484696637531
12	0.06109802539492486
13	0.060559377815876406
14	0.06502245775656361
15	0.05802003922893369
16	0.06256006882377067
17	0.05855868680798214
18	0.07287132247984111
19	0.0714092790509953
20	0.07648795622488075
21	0.07210182593834331
22	0.07425641625453713
23	0.07910424446597325
24	0.0744103155628367
25	0.07456421487113626
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1299551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.74549674278528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.47882719882426	48.08247508270535
2	12.41549172778105	14.835394411644407
3	3.335729739058846	5.978844907792568
4	1.3085816437824755	3.1272744134509796
5	0.6790578413515738	2.0285324024316624
6	0.41390139731218867	1.4837246750969786
7	0.25442724742050427	1.0640617597426294
8	0.17471859699876588	0.8350919494315024
9	0.13379808674568533	0.7194449840269721
>10	0.6727196673784148	7.28614871028665
>50	0.05913859332688339	2.472270488544
>100	0.06548688455062388	8.012696499404495
>500	0.005929893199330291	2.3818695245430717
>1k	0.0021914822693177164	1.6921701908987852
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3022	0.23254185484063344	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1988	0.1529759124497615	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1356	0.10434373102710089	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1335	0.10272778828995553	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.694965414977943E-5	0.0
8	0.0	0.0	0.0	7.694965414977943E-5	0.0
9	0.0	0.0	0.0	7.694965414977943E-5	0.0
10	0.0	0.0	0.0	7.694965414977943E-5	0.0
11	0.0	0.0	0.0	7.694965414977943E-5	0.0
12	0.0	0.0	0.0	7.694965414977943E-5	7.694965414977943E-5
13	0.0	0.0	0.0	7.694965414977943E-5	2.3084896244933827E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	95	4.8985385E-9	13.999971	7
CGAACGA	130	1.8189894E-12	13.888331	16
ACGAACG	135	1.8189894E-12	13.373949	15
CGCGGTC	105	1.9934305E-8	12.666154	10
ACTAGAC	60	4.1046028E-4	12.661764	3
CGTTATT	75	1.4863857E-5	12.659815	2
AACGAGA	145	7.2759576E-12	12.452087	18
CCGACCA	215	0.0	12.372544	9
GCGTAAC	70	1.09104636E-4	12.213791	11
GCGTTAT	70	1.0945819E-4	12.2095585	1
ACCGTCT	55	0.003068024	12.090884	8
CGACCAT	220	0.0	12.090419	10
GCTCGAC	55	0.0030689093	12.090419	11
GATATAC	55	0.0030768851	12.08623	1
GATAACG	150	1.4551915E-11	12.0328455	11
GAACCGC	120	1.0017175E-8	11.874062	6
TAAGACT	105	2.7265742E-7	11.759163	4
TACACCG	65	8.0297765E-4	11.690933	5
TCGAACG	65	8.0479536E-4	11.687782	3
ACGAGAC	155	2.5465852E-11	11.648278	19
>>END_MODULE