FastQCFastQC Report
Thu 2 Feb 2017
SRR4063945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299551
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA2050.021.461971
GTATCAA19650.020.2632661
ATACGAA4050.019.01106540
CGAATGC3850.018.856643
CTAGCGG4400.018.0022529
CTATACT1355.5355486E-817.923344
GCATTCG4100.017.70748722
TTGTTCG750.001289193917.60355636
TAGCGGC4550.017.4094430
CGCAATA4300.017.39886536
TACGAAT4300.017.39350941
TCTACAC3050.017.3089793
CAATACG4500.017.11325338
AATACGA4550.016.92258839
GTACTAA1053.7768754E-516.7607771
AACGCAG23500.016.7550286
GTATAAG2251.8189894E-1216.6211031
GTATTAC1602.1313099E-816.4988881
CCGTTAA800.001990377416.49761816
CGGTCCA5150.016.23191610
Produced by FastQC (version 0.11.5)