##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1299551
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12775104632292	32.0	32.0	32.0	32.0	32.0
2	30.94610600122658	32.0	32.0	32.0	32.0	32.0
3	31.212695000042324	32.0	32.0	32.0	32.0	32.0
4	31.384645927708878	32.0	32.0	32.0	32.0	32.0
5	31.239159525097513	32.0	32.0	32.0	32.0	32.0
6	34.83934066458338	36.0	36.0	36.0	32.0	36.0
7	34.880368681182965	36.0	36.0	36.0	32.0	36.0
8	34.794151980183926	36.0	36.0	36.0	32.0	36.0
9	34.98761957014384	36.0	36.0	36.0	36.0	36.0
10	34.73532089160025	36.0	36.0	36.0	32.0	36.0
11	34.99708668609389	36.0	36.0	36.0	36.0	36.0
12	34.853022313091216	36.0	36.0	36.0	32.0	36.0
13	34.929568751053246	36.0	36.0	36.0	32.0	36.0
14	34.86507570691724	36.0	36.0	36.0	32.0	36.0
15	34.79306545106733	36.0	36.0	36.0	32.0	36.0
16	34.8157986873928	36.0	36.0	36.0	32.0	36.0
17	34.78000555576503	36.0	36.0	36.0	32.0	36.0
18	34.78146221271808	36.0	36.0	36.0	32.0	36.0
19	34.784626382496725	36.0	36.0	36.0	32.0	36.0
20	34.78428164804613	36.0	36.0	36.0	32.0	36.0
21	34.7749222616119	36.0	36.0	36.0	32.0	36.0
22	34.76180080658627	36.0	36.0	36.0	32.0	36.0
23	34.69573798950561	36.0	36.0	36.0	32.0	36.0
24	34.66827773592571	36.0	36.0	36.0	32.0	36.0
25	34.62794611369619	36.0	36.0	36.0	32.0	36.0
26	34.56059131192235	36.0	36.0	36.0	32.0	36.0
27	34.563153735405535	36.0	36.0	36.0	32.0	36.0
28	34.53384976811222	36.0	36.0	36.0	32.0	36.0
29	34.50448885807483	36.0	36.0	36.0	32.0	36.0
30	34.47467702306412	36.0	36.0	36.0	32.0	36.0
31	34.47231082119902	36.0	36.0	36.0	32.0	36.0
32	34.429742272523356	36.0	36.0	36.0	32.0	36.0
33	34.406858214875754	36.0	36.0	36.0	32.0	36.0
34	34.388898935093735	36.0	36.0	36.0	32.0	36.0
35	34.35708794806822	36.0	36.0	36.0	32.0	36.0
36	34.31860850401408	36.0	36.0	36.0	32.0	36.0
37	34.31298425379227	36.0	36.0	36.0	32.0	36.0
38	34.27439554122924	36.0	36.0	36.0	32.0	36.0
39	34.271391426731235	36.0	36.0	36.0	32.0	36.0
40	34.25216940312462	36.0	36.0	36.0	32.0	36.0
41	34.22854047282485	36.0	36.0	36.0	32.0	36.0
42	34.152720439597985	36.0	36.0	36.0	32.0	36.0
43	34.15177626734157	36.0	36.0	36.0	32.0	36.0
44	34.07975985551933	36.0	36.0	36.0	32.0	36.0
45	34.040907975139106	36.0	36.0	36.0	32.0	36.0
46	34.03707434336936	36.0	36.0	36.0	32.0	36.0
47	34.00830671516547	36.0	36.0	36.0	32.0	36.0
48	33.95886733187078	36.0	36.0	36.0	32.0	36.0
49	33.94168986057492	36.0	36.0	36.0	32.0	36.0
50	33.2233271337562	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	2.0
20	9.0
21	40.0
22	128.0
23	368.0
24	990.0
25	2373.0
26	4922.0
27	9207.0
28	15650.0
29	25139.0
30	38350.0
31	58330.0
32	88922.0
33	149591.0
34	294838.0
35	610689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03759088362587	18.45991885885257	12.019494191451246	26.48299606607032
2	16.1256712905785	20.488877295123153	36.50805010045793	26.877401313840416
3	18.195634174343695	24.28036517312173	28.940370036351098	28.583630616183477
4	12.390664160159933	15.9431988432928	36.31692792356745	35.34920907297982
5	14.437909708814814	36.78785980696409	33.283572556983145	15.490657927237947
6	34.508022007618024	36.06756184833211	16.288715324535417	13.135700819514447
7	30.222230772190372	30.533338463314223	20.78319418260167	18.461236581893733
8	28.176116212445685	33.159529714493694	19.00933476254491	19.65501931051571
9	27.24920144759026	13.908393668831792	18.081334996487218	40.76106988709073
10	15.268556566169567	26.937839543222985	32.0200994197947	25.773504470812746
11	37.722413356613174	20.902373204283634	22.314553257240384	19.06066018186281
12	24.537589579153998	23.57233163197386	28.953275328594767	22.93680346027737
13	29.549020467870008	19.392650835020454	25.20135831738549	25.856970379724043
14	23.346673879436448	19.33735370863847	25.24687448780229	32.069097924122794
15	25.13805352228864	27.159606493594442	22.1916804668887	25.51065951722822
16	25.765151829946763	25.796085709932548	23.56776905165342	24.870993408467264
17	24.114174818841278	25.93695822634125	25.006867756632868	24.941999198184604
18	24.91023788307609	25.062752684399015	25.961835902692172	24.065173529832727
19	25.484302631143574	25.043688233379424	25.452368475524974	24.019640659952028
20	25.81603812423002	24.29288494937828	24.924646471331034	24.96643045506067
21	26.647126584489566	24.30524081009518	24.505079061922157	24.5425535434931
22	25.735369935746988	24.392597437574544	24.880997268285178	24.99103535839329
23	24.37816458131339	24.25850685627222	25.463702540899085	25.899626021515303
24	24.821803838402648	24.97462585154411	25.127601763993873	25.075968546059368
25	24.975625671881144	24.47613470481092	25.373310052996366	25.174929570311566
26	24.789666028206643	25.12780102210036	25.45800977467158	24.624523175021412
27	25.263762191546373	24.826890430376785	24.772483466285383	25.13686391179146
28	24.521329603650678	24.789435814957464	25.584673928517837	25.104560652874024
29	24.675650468940937	24.86849030875921	25.460322394969236	24.99553682733062
30	24.663063234993096	24.98988073892922	25.684993174308314	24.66206285176937
31	25.13474993920477	24.801453544746828	24.802146148660505	25.2616503673879
32	24.89511415089403	24.856099385305825	24.773914435309365	25.47487202849078
33	24.49422465736558	24.615349098492487	25.275454216654225	25.61497202748771
34	25.192533220060394	24.552448757845227	25.436931903878058	24.81808611821632
35	25.794762325433933	24.579379272770925	25.193688688334355	24.432169713460787
36	24.84012565238738	25.121935453088902	24.995190302418393	25.042748592105323
37	25.57027834616009	24.927260316876453	24.81652516319776	24.685936173765697
38	24.732097164558716	24.787197611287894	25.230617686781663	25.250087537371723
39	25.161756014676552	24.810925533748676	24.82570058361447	25.2016178679603
40	25.409941401215068	24.866430937695984	25.245726290192877	24.477901370896067
41	24.591074748519436	25.15975325970524	25.50665484677231	24.742517145003017
42	25.697776334510948	25.255690365911747	24.948269400802296	24.09826389877501
43	24.977568813340206	24.536024562726524	25.265749924972873	25.220656698960394
44	24.744233863551894	25.018410544759746	24.887055059263872	25.35030053242449
45	24.872357403886184	25.317211570055875	24.8738964253917	24.936534600666242
46	24.699776996985072	24.887305757294538	25.113078996009342	25.299838249711048
47	24.855235121248523	24.782823825086318	25.383121934928333	24.97881911873683
48	25.46657217103709	25.29350973543532	24.641505281520796	24.598412812006792
49	24.680887083121718	25.35958877756745	24.859719593086783	25.099804546224046
50	24.845232864421295	25.354723581471188	24.823994465704217	24.976049088403297
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	15.5
2	19.0
3	56.0
4	93.0
5	127.5
6	162.0
7	166.0
8	170.0
9	224.0
10	278.0
11	434.5
12	591.0
13	1064.5
14	1538.0
15	2057.5
16	2577.0
17	2745.0
18	2913.0
19	3195.5
20	3478.0
21	4156.0
22	4834.0
23	5595.5
24	6357.0
25	7483.5
26	8610.0
27	10338.5
28	12067.0
29	14179.0
30	16291.0
31	18350.0
32	20409.0
33	23846.0
34	27283.0
35	31015.0
36	34747.0
37	41016.5
38	47286.0
39	51979.5
40	56673.0
41	62549.5
42	68426.0
43	70660.5
44	72895.0
45	79627.0
46	86359.0
47	91281.5
48	96204.0
49	98938.5
50	101673.0
51	99152.5
52	96632.0
53	94951.0
54	93270.0
55	93061.0
56	92852.0
57	89896.0
58	86940.0
59	78593.0
60	70246.0
61	62217.0
62	54188.0
63	47273.0
64	40358.0
65	34524.0
66	28690.0
67	24734.0
68	20778.0
69	18727.5
70	16677.0
71	13065.0
72	9453.0
73	7982.5
74	6512.0
75	5201.5
76	3891.0
77	3339.0
78	2787.0
79	2281.0
80	1775.0
81	1448.0
82	1121.0
83	914.5
84	708.0
85	521.5
86	335.0
87	245.5
88	156.0
89	120.0
90	84.0
91	67.5
92	51.0
93	42.5
94	34.0
95	33.5
96	33.0
97	27.0
98	21.0
99	17.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0066946199110308096
2	1.5389930829955885E-4
3	2.3084896244933827E-4
4	0.0
5	0.0
6	7.694965414977943E-5
7	7.694965414977943E-5
8	0.0
9	9.233958497973531E-4
10	8.464461956475737E-4
11	0.0
12	1.5389930829955885E-4
13	1.5389930829955885E-4
14	3.077986165991177E-4
15	0.004232230978237868
16	3.847482707488971E-4
17	0.0
18	3.847482707488971E-4
19	1.5389930829955885E-4
20	7.694965414977942E-4
21	0.0
22	7.694965414977943E-5
23	8.464461956475737E-4
24	0.0
25	0.0021545903161938242
26	0.00538647579048456
27	0.006540720602731251
28	0.005078677173885442
29	0.0020776406620440445
30	0.0037705330533391915
31	0.008233612994026399
32	0.0033088351284405156
33	0.004693928903136545
34	0.00269323789524228
35	0.0033088351284405156
36	0.007156317835929487
37	0.004155281324088089
38	0.008156663339876618
39	0.004847828211436104
40	0.0027701875493920595
41	0.0036166337450396326
42	0.0023084896244933827
43	0.001615942737145368
44	0.0018467916995947061
45	0.0018467916995947061
46	0.0013081441205462503
47	0.0023084896244933827
48	0.0015389930829955885
49	8.464461956475737E-4
50	0.0013850937746960298
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1299551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.93807166513204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.26885534427238	52.60111968810469
2	11.36546306218885	14.533715835552838
3	3.0908848868904317	5.928756582200219
4	1.145754921003987	2.9302944099932255
5	0.6194836112692694	1.980429376635468
6	0.35989544318950933	1.3806612383163184
7	0.2148889753215424	0.9617710692908901
8	0.1507906649148711	0.7713011471807951
9	0.11265113903389512	0.6482426940637147
>10	0.5642644697972999	6.517646694097684
>50	0.0486873266250421	2.1809127694342623
>100	0.05269930079389763	6.84970958937222
>500	0.004713900707765742	2.0144187186578693
>1k	9.669539913365625E-4	0.7010201870998822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	7.694965414977943E-5
6	0.0	0.0	0.0	0.0	7.694965414977943E-5
7	0.0	0.0	0.0	0.0	7.694965414977943E-5
8	0.0	0.0	0.0	7.694965414977943E-5	7.694965414977943E-5
9	0.0	0.0	0.0	7.694965414977943E-5	7.694965414977943E-5
10	0.0	0.0	0.0	7.694965414977943E-5	7.694965414977943E-5
11	0.0	0.0	0.0	7.694965414977943E-5	7.694965414977943E-5
12	0.0	0.0	0.0	7.694965414977943E-5	2.3084896244933827E-4
13	0.0	0.0	0.0	7.694965414977943E-5	4.6169792489867653E-4
14	0.0	0.0	0.0	1.5389930829955885E-4	4.6169792489867653E-4
15	0.0	0.0	0.0	1.5389930829955885E-4	6.155972331982354E-4
16	0.0	0.0	0.0	1.5389930829955885E-4	6.925468873480148E-4
17	0.0	0.0	0.0	2.3084896244933827E-4	6.925468873480148E-4
18	0.0	0.0	0.0	2.3084896244933827E-4	6.925468873480148E-4
19	0.0	0.0	0.0	2.3084896244933827E-4	7.694965414977942E-4
20	0.0	0.0	0.0	2.3084896244933827E-4	0.0010003455039471324
21	0.0	0.0	0.0	3.077986165991177E-4	0.0010003455039471324
22	0.0	0.0	0.0	3.847482707488971E-4	0.0010003455039471324
23	0.0	0.0	0.0	0.0012311944663964708	0.0010003455039471324
24	0.0	0.0	0.0	0.001615942737145368	0.0010003455039471324
25	0.0	0.0	0.0	0.002154590316193824	0.0010003455039471324
26	0.0	0.0	0.0	0.0026162882410925006	0.0010003455039471324
27	0.0	0.0	0.0	0.003077986165991177	0.001077295158096912
28	0.0	0.0	0.0	0.005232576482185001	0.001077295158096912
29	0.0	0.0	0.0	0.011773297084916251	0.001077295158096912
30	0.0	0.0	0.0	0.022392349357585814	0.001077295158096912
31	0.0	0.0	0.0	0.04547724560251964	0.001077295158096912
32	0.0	0.0	0.0	0.06925468873480148	0.0011542448122466914
33	0.0	0.0	0.0	0.09180093740068686	0.0011542448122466914
34	0.0	0.0	0.0	0.11819466877406119	0.0011542448122466914
35	0.0	0.0	0.0	0.15074437247941788	0.0011542448122466914
36	0.0	0.0	0.0	0.19175853814125032	0.0011542448122466914
37	0.0	0.0	0.0	0.25039417460338226	0.0011542448122466914
38	0.0	0.0	0.0	0.3358082907096374	0.0011542448122466914
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATATA	205	0.0	21.46197	1
GTATCAA	1965	0.0	20.263266	1
ATACGAA	405	0.0	19.011065	40
CGAATGC	385	0.0	18.8566	43
CTAGCGG	440	0.0	18.00225	29
CTATACT	135	5.5355486E-8	17.92334	4
GCATTCG	410	0.0	17.707487	22
TTGTTCG	75	0.0012891939	17.603556	36
TAGCGGC	455	0.0	17.40944	30
CGCAATA	430	0.0	17.398865	36
TACGAAT	430	0.0	17.393509	41
TCTACAC	305	0.0	17.308979	3
CAATACG	450	0.0	17.113253	38
AATACGA	455	0.0	16.922588	39
GTACTAA	105	3.7768754E-5	16.760777	1
AACGCAG	2350	0.0	16.755028	6
GTATAAG	225	1.8189894E-12	16.621103	1
GTATTAC	160	2.1313099E-8	16.498888	1
CCGTTAA	80	0.0019903774	16.497618	16
CGGTCCA	515	0.0	16.231916	10
>>END_MODULE