FastQCFastQC Report
Thu 2 Feb 2017
SRR4063944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486856
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31870.2143448995733279No Hit
TATCAACGCAGAGTACTTTTTTTTT22370.15045169135410558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1058.913048E-1114.4711662
GCGTTAT1203.6379788E-1214.2469711
GTCCTAA1750.014.1112861
ACGCGCG801.9979598E-613.0632413
ATTAGAC802.0062816E-613.0584053
ATAATAC1102.757588E-912.9504853
TCGCGTA1501.8189894E-1212.6678119
CGAACGA1155.318725E-912.39200716
ACGCCAC2300.012.39200716
AACGCCA2150.012.37279415
AGACGAT853.9419665E-612.29447
CGCCACT2250.012.24637517
GTATTAG2650.012.1860891
GTATCAA22750.011.9403191
CGCATCG1850.011.811480513
AAGACGA1052.7197348E-711.761786
GTCCTAC2950.011.5907551
CGAGCCG2800.011.53636815
CGCGTAA1657.2759576E-1211.51580410
GCGTAAC1657.2759576E-1211.51541711
Produced by FastQC (version 0.11.5)