##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063944_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1486856
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29423360433021	32.0	32.0	32.0	32.0	32.0
2	30.96340129777194	32.0	32.0	32.0	32.0	32.0
3	30.977175328343833	32.0	32.0	32.0	32.0	32.0
4	31.009052658764535	32.0	32.0	32.0	32.0	32.0
5	30.92778924119081	32.0	32.0	32.0	32.0	32.0
6	34.56308344587505	36.0	36.0	36.0	32.0	36.0
7	34.52202028979269	36.0	36.0	36.0	32.0	36.0
8	34.485376526038834	36.0	36.0	36.0	32.0	36.0
9	34.599669369461466	36.0	36.0	36.0	32.0	36.0
10	34.36582224505938	36.0	36.0	36.0	32.0	36.0
11	34.56453079518124	36.0	36.0	36.0	32.0	36.0
12	34.420097844041386	36.0	36.0	36.0	32.0	36.0
13	34.49299663181909	36.0	36.0	36.0	32.0	36.0
14	34.40567546554609	36.0	36.0	36.0	32.0	36.0
15	34.351724713085865	36.0	36.0	36.0	32.0	36.0
16	34.348390832736996	36.0	36.0	36.0	32.0	36.0
17	34.28220688486309	36.0	36.0	36.0	32.0	36.0
18	34.271041714866804	36.0	36.0	36.0	32.0	36.0
19	34.27431506480789	36.0	36.0	36.0	32.0	36.0
20	34.254478577616126	36.0	36.0	36.0	32.0	36.0
21	34.232918318922614	36.0	36.0	36.0	32.0	36.0
22	34.21815495246345	36.0	36.0	36.0	32.0	36.0
23	34.172571520039604	36.0	36.0	36.0	32.0	36.0
24	34.15290855335016	36.0	36.0	36.0	32.0	36.0
25	33.75854958381982	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	14.0
5	17.0
6	52.0
7	17.0
8	83.0
9	81.0
10	112.0
11	37.0
12	96.0
13	71.0
14	139.0
15	254.0
16	391.0
17	556.0
18	721.0
19	938.0
20	1324.0
21	1870.0
22	2815.0
23	4237.0
24	6561.0
25	9509.0
26	13985.0
27	18592.0
28	26462.0
29	36788.0
30	49576.0
31	68583.0
32	98539.0
33	143455.0
34	314398.0
35	686576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24757857683023	17.184838217445012	11.35902459503901	26.20855861068575
2	16.710642868624937	19.794537086298057	37.62457214843532	25.87024789664169
3	18.727927077217146	23.922996059409495	28.481880614702526	28.867196248670833
4	12.590075638650907	15.745766428168839	35.844622634022194	35.81953529915806
5	14.590157539102277	36.64838956590989	33.64174819840968	15.119704696578157
6	34.044054487276505	35.335204643432014	17.241815372590487	13.378925496700992
7	30.331128678983312	30.386558963292238	20.84030696538029	18.442005392344164
8	28.160285042085555	32.833863817860475	19.39057184011862	19.615279299935345
9	27.790169484910937	14.21865969052805	18.36020371293674	39.63096711162427
10	15.954881824537887	26.615605230769024	31.614215643721995	25.815297300971096
11	36.96703225715284	20.980977586133807	22.765460211333238	19.28652994538012
12	25.151853671339257	23.533113050734535	28.731468333815624	22.58356494411058
13	29.623161603884945	19.61758938101218	25.012416366183878	25.746832648919003
14	23.728293414684867	19.686694299806785	25.266488861610192	31.31852342389816
15	25.14851871591539	27.20234345979295	22.22020339056048	25.428934433731175
16	25.73591168252225	25.62379198768164	23.819243669880855	24.821052659915257
17	24.093193466416864	25.73627979843659	25.287351680771796	24.883175054374746
18	24.686302580452796	25.020747139317802	26.136818718126303	24.156131562103102
19	25.678117766783082	24.837541821833437	25.3324377240846	24.151902687298886
20	25.90294179213274	24.43625242053217	24.77453798712684	24.88626780020825
21	26.932605724000673	24.17930257460901	24.323873200523096	24.56421850086722
22	26.04603672141652	24.393983551486915	24.686231050522743	24.873748676573825
23	24.598764185614474	24.318552024016263	25.28075064281195	25.801933147557314
24	24.874424452417923	24.844406125084888	25.15845436574842	25.122715056748774
25	24.860508160861517	24.630927141174492	25.25121992259802	25.257344775365976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	113.0
1	113.0
2	295.5
3	478.0
4	478.0
5	478.0
6	1258.5
7	2039.0
8	2039.0
9	2039.0
10	2260.0
11	2481.0
12	2481.0
13	2481.0
14	3133.0
15	3785.0
16	3785.0
17	3785.0
18	6563.0
19	9341.0
20	9341.0
21	9341.0
22	15221.0
23	21101.0
24	21101.0
25	21101.0
26	31580.5
27	42060.0
28	42060.0
29	42060.0
30	52886.0
31	63712.0
32	63712.0
33	63712.0
34	78934.5
35	94157.0
36	94157.0
37	94157.0
38	107493.0
39	120829.0
40	120829.0
41	120829.0
42	138961.0
43	157093.0
44	157093.0
45	157093.0
46	175091.0
47	193089.0
48	193089.0
49	193089.0
50	197082.5
51	201076.0
52	201076.0
53	201076.0
54	188571.5
55	176067.0
56	176067.0
57	176067.0
58	161322.0
59	146577.0
60	146577.0
61	146577.0
62	127782.0
63	108987.0
64	108987.0
65	108987.0
66	89281.0
67	69575.0
68	69575.0
69	69575.0
70	52581.5
71	35588.0
72	35588.0
73	35588.0
74	27198.0
75	18808.0
76	18808.0
77	18808.0
78	15023.5
79	11239.0
80	11239.0
81	11239.0
82	7773.0
83	4307.0
84	4307.0
85	4307.0
86	3301.5
87	2296.0
88	2296.0
89	2296.0
90	1636.5
91	977.0
92	977.0
93	977.0
94	655.0
95	333.0
96	333.0
97	333.0
98	540.5
99	748.0
100	748.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03631824467197899
2	0.004506152579671468
3	0.0015468881989916979
4	0.0033628004325906478
5	0.007667184986306676
6	0.01324943370440715
7	0.02044582663015114
8	0.032215628144218404
9	0.04351463759772298
10	0.05400657494740581
11	0.05158535863594054
12	0.059588823665506274
13	0.06147199190775703
14	0.06449851229708863
15	0.05703309533673739
16	0.06348967216731143
17	0.0586472395443809
18	0.07458691359486057
19	0.07324179342182431
20	0.07593203376789683
21	0.07243472131800255
22	0.07593203376789683
23	0.08043818634756829
24	0.0737798414910388
25	0.07438514556890513
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1486856.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.83730930823794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.02535495966256	46.284512073035366
2	12.39091322315031	14.333141613977599
3	3.4736262851964192	6.027155936343924
4	1.4337056558979484	3.3168670990855924
5	0.7189057888754645	2.078978823733652
6	0.4229783032561914	1.4678356173661207
7	0.2905761830120885	1.1764301202134122
8	0.19945112601232884	0.9228573173641125
9	0.14408750094105321	0.7500270023440848
>10	0.7504441712018503	7.825534813825899
>50	0.07030214474816616	2.8664379697744793
>100	0.07007750077839375	8.423976605004183
>500	0.00759164905337169	2.9428529776962997
>1k	0.00198550821395875	1.58339203023531
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3187	0.2143448995733279	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2237	0.15045169135410558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.725600865181296E-5
2	0.0	0.0	0.0	0.0	2.0176802595543887E-4
3	0.0	0.0	0.0	0.0	2.0176802595543887E-4
4	0.0	0.0	0.0	6.725600865181296E-5	2.0176802595543887E-4
5	0.0	0.0	0.0	6.725600865181296E-5	2.0176802595543887E-4
6	0.0	0.0	0.0	6.725600865181296E-5	2.6902403460725183E-4
7	0.0	0.0	0.0	1.3451201730362591E-4	2.6902403460725183E-4
8	0.0	0.0	0.0	1.3451201730362591E-4	2.6902403460725183E-4
9	0.0	0.0	0.0	1.3451201730362591E-4	2.6902403460725183E-4
10	0.0	0.0	0.0	1.3451201730362591E-4	3.362800432590648E-4
11	0.0	0.0	0.0	1.3451201730362591E-4	3.362800432590648E-4
12	0.0	0.0	0.0	1.3451201730362591E-4	6.053040778663166E-4
13	0.0	0.0	0.0	1.3451201730362591E-4	6.053040778663166E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	105	8.913048E-11	14.471166	2
GCGTTAT	120	3.6379788E-12	14.246971	1
GTCCTAA	175	0.0	14.111286	1
ACGCGCG	80	1.9979598E-6	13.06324	13
ATTAGAC	80	2.0062816E-6	13.058405	3
ATAATAC	110	2.757588E-9	12.950485	3
TCGCGTA	150	1.8189894E-12	12.667811	9
CGAACGA	115	5.318725E-9	12.392007	16
ACGCCAC	230	0.0	12.392007	16
AACGCCA	215	0.0	12.372794	15
AGACGAT	85	3.9419665E-6	12.2944	7
CGCCACT	225	0.0	12.246375	17
GTATTAG	265	0.0	12.186089	1
GTATCAA	2275	0.0	11.940319	1
CGCATCG	185	0.0	11.8114805	13
AAGACGA	105	2.7197348E-7	11.76178	6
GTCCTAC	295	0.0	11.590755	1
CGAGCCG	280	0.0	11.536368	15
CGCGTAA	165	7.2759576E-12	11.515804	10
GCGTAAC	165	7.2759576E-12	11.515417	11
>>END_MODULE