FastQCFastQC Report
Thu 2 Feb 2017
SRR4063944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486856
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26250.020.4504451
CCGTTAT600.007408080618.33539231
CGAATTT600.00740951818.33477828
GTATTAA2300.018.1747321
CGTCGTA4150.018.02248610
ATACCGT4550.017.4044516
CGAGCCG5100.017.25393915
TCTATAC902.2178865E-417.1090073
TAGGACG3400.016.8214594
TACCGTC4450.016.806927
ATCCCGA1053.7781785E-516.76040616
CGCAATA3900.016.36081336
ACCGTCG4600.016.2588678
TAGGACA3850.015.9980314
AACGCAG33450.015.9800976
CCGTCGT4850.015.8748689
GGTATCA14850.015.8525671
GGTATAT1259.439069E-615.8407141
GTCGTAG4600.015.78119711
TAGCGGC4050.015.75432730
Produced by FastQC (version 0.11.5)