##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1486856
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323344022554974	32.0	32.0	32.0	32.0	32.0
2	31.360909193627357	32.0	32.0	32.0	32.0	32.0
3	31.4451950962299	32.0	32.0	32.0	32.0	32.0
4	31.53865539097263	32.0	32.0	32.0	32.0	32.0
5	31.468858450313952	32.0	32.0	32.0	32.0	32.0
6	35.06238936386577	36.0	36.0	36.0	36.0	36.0
7	35.081931942299725	36.0	36.0	36.0	36.0	36.0
8	35.03160225334531	36.0	36.0	36.0	36.0	36.0
9	35.15257160074681	36.0	36.0	36.0	36.0	36.0
10	34.9889155372141	36.0	36.0	36.0	36.0	36.0
11	35.1474487105678	36.0	36.0	36.0	36.0	36.0
12	35.05059602274867	36.0	36.0	36.0	36.0	36.0
13	35.0945296652803	36.0	36.0	36.0	36.0	36.0
14	35.035237440612946	36.0	36.0	36.0	36.0	36.0
15	35.00523857051389	36.0	36.0	36.0	36.0	36.0
16	35.02026759820723	36.0	36.0	36.0	36.0	36.0
17	34.980113743361834	36.0	36.0	36.0	36.0	36.0
18	34.97473393522977	36.0	36.0	36.0	36.0	36.0
19	34.975001614144205	36.0	36.0	36.0	36.0	36.0
20	34.97036363978758	36.0	36.0	36.0	36.0	36.0
21	34.97240149684973	36.0	36.0	36.0	36.0	36.0
22	34.94833931463437	36.0	36.0	36.0	36.0	36.0
23	34.902839279661244	36.0	36.0	36.0	32.0	36.0
24	34.87514930833921	36.0	36.0	36.0	32.0	36.0
25	34.84577390144035	36.0	36.0	36.0	32.0	36.0
26	34.77650559300968	36.0	36.0	36.0	32.0	36.0
27	34.7795025207552	36.0	36.0	36.0	32.0	36.0
28	34.754550541545385	36.0	36.0	36.0	32.0	36.0
29	34.72217618922075	36.0	36.0	36.0	32.0	36.0
30	34.70379781229655	36.0	36.0	36.0	32.0	36.0
31	34.70168799130514	36.0	36.0	36.0	32.0	36.0
32	34.65587857869222	36.0	36.0	36.0	32.0	36.0
33	34.63655929020699	36.0	36.0	36.0	32.0	36.0
34	34.62434963439634	36.0	36.0	36.0	32.0	36.0
35	34.582211727295714	36.0	36.0	36.0	32.0	36.0
36	34.5738766901435	36.0	36.0	36.0	32.0	36.0
37	34.55323514852817	36.0	36.0	36.0	32.0	36.0
38	34.51658129637302	36.0	36.0	36.0	32.0	36.0
39	34.51602710686173	36.0	36.0	36.0	32.0	36.0
40	34.49612739902183	36.0	36.0	36.0	32.0	36.0
41	34.48120261814191	36.0	36.0	36.0	32.0	36.0
42	34.41393584852871	36.0	36.0	36.0	32.0	36.0
43	34.40423954976138	36.0	36.0	36.0	32.0	36.0
44	34.34589428969584	36.0	36.0	36.0	32.0	36.0
45	34.31892732046681	36.0	36.0	36.0	32.0	36.0
46	34.29092595382471	36.0	36.0	36.0	32.0	36.0
47	34.259366744324936	36.0	36.0	36.0	32.0	36.0
48	34.223902650962835	36.0	36.0	36.0	32.0	36.0
49	34.20403388088692	36.0	36.0	36.0	32.0	36.0
50	33.57667723034376	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	15.0
21	30.0
22	164.0
23	441.0
24	1090.0
25	2533.0
26	5314.0
27	9852.0
28	16687.0
29	26085.0
30	37895.0
31	54584.0
32	79935.0
33	130446.0
34	270889.0
35	850886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28609584844186	18.162789399378372	11.908715897771168	26.642398854408594
2	15.920963163087626	20.436412256507207	37.19572438663995	26.44690019376521
3	17.993629502291416	24.547063800480746	28.79395017937202	28.665356517855823
4	12.28666394055645	16.3061520416234	36.07585401679786	35.331330001022295
5	14.327883803138972	37.03183092377473	33.383865014500394	15.256420258585901
6	34.10234112227789	35.79329737815019	16.923820880505176	13.180540619066738
7	30.29833460447361	30.552293634748267	20.83345103150679	18.315920729271333
8	28.181814513308616	32.97669713812232	19.294134737997492	19.54735361057157
9	27.807056835897832	14.072847014006868	18.311427845056837	39.808668305038466
10	15.853848823413887	26.874238319554706	31.588115498330694	25.683797358700712
11	37.12289555948928	21.04084053869373	22.67697746116638	19.159286440650607
12	25.09166993979242	23.617149206110074	28.713675029727153	22.577505824370352
13	29.674028768220577	19.538500547130205	25.15453822143338	25.632932463215834
14	23.7505498186103	19.773293141088498	25.18081802248237	31.29533901781883
15	25.180422251965645	27.29329629604719	22.15908097310313	25.36720047888404
16	25.7249564178719	25.730605952995866	23.72656788050964	24.81786974862259
17	24.266102433591417	25.784137804871488	25.14877029113781	24.800989470399283
18	24.878148516562856	25.045213003858493	26.0059010620432	24.070737417535447
19	25.817429529154133	24.800249654304114	25.406159036248297	23.976161780293452
20	26.001599357566718	24.405066560311855	24.749688434654	24.843645647467426
21	27.0645576975847	24.114641902107532	24.313921455742857	24.506878944564907
22	26.13555045007721	24.40243036312864	24.736020166041634	24.725999020752514
23	24.710663872396175	24.221980398856108	25.339845525983755	25.72751020276396
24	25.11124203684573	24.80347688533058	25.10317127238292	24.98210980544077
25	25.05757238247432	24.425352294555374	25.281808741317064	25.235266581653242
26	24.729144751179064	25.244287624464274	25.476401223590432	24.550166400766223
27	25.46612634729532	24.82837012997932	24.69721376805502	25.008289754670344
28	24.777996218722233	24.71154473469514	25.447555453023575	25.06290359355905
29	24.835503638323903	24.90013861882916	25.157400638009808	25.10695710483713
30	24.672046467706394	24.939467149494618	25.50417138598705	24.884314996811938
31	25.31843010276799	24.7116691676526	24.691894451443318	25.27800627813609
32	25.03309093550496	24.865954808005316	24.601967161954573	25.498987094535153
33	24.709037982448116	24.534164832740558	25.153753853935186	25.60304333087614
34	25.335933953222472	24.572831368856455	25.40420022531233	24.687034452608746
35	25.75349338986608	24.59026316780647	25.155904461100558	24.500338981226896
36	24.760432704240074	24.98938955824914	25.051942400266892	25.198235337243897
37	25.62230882121493	24.899414985990063	24.6473975687415	24.830878624053508
38	24.95900431951206	24.792801979092538	25.176526929352903	25.071666772042494
39	25.41666778764538	24.662899281407803	24.614069448666797	25.306363482280013
40	25.74658837930362	24.728180087837885	25.10596369457167	24.419267838286824
41	24.787228461547777	25.03783277710744	25.402574377558334	24.772364383786453
42	25.75891172714084	25.24378998851922	24.805408542855854	24.19188974148408
43	25.145510527738807	24.554119693254258	25.065138408188137	25.235231370818802
44	24.80741008771635	24.95490430026674	24.82065968182142	25.417025930195486
45	24.983673373306388	25.13439621746865	24.906865926615932	24.97506448260903
46	24.874011071825628	24.807897570480154	24.986733582766636	25.331357774927582
47	25.106603354810943	24.67844123700246	25.256184339731774	24.958771068454823
48	25.56287462201513	25.249254791395398	24.48138054601999	24.706490040569477
49	24.944412684534417	25.25863402495208	24.64142314288597	25.155530147627537
50	24.910497561592408	25.467924733880317	24.58430043495192	25.03727726957536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	21.0
2	22.0
3	72.0
4	122.0
5	145.5
6	169.0
7	164.5
8	160.0
9	238.0
10	316.0
11	461.5
12	607.0
13	1155.0
14	1703.0
15	2317.0
16	2931.0
17	3238.0
18	3545.0
19	3651.5
20	3758.0
21	4198.0
22	4638.0
23	5325.5
24	6013.0
25	7254.0
26	8495.0
27	10780.0
28	13065.0
29	15563.0
30	18061.0
31	20668.0
32	23275.0
33	27492.0
34	31709.0
35	37189.5
36	42670.0
37	49542.0
38	56414.0
39	60955.0
40	65496.0
41	71475.0
42	77454.0
43	79256.0
44	81058.0
45	88450.5
46	95843.0
47	102276.5
48	108710.0
49	112473.5
50	116237.0
51	113148.5
52	110060.0
53	108921.5
54	107783.0
55	107798.5
56	107814.0
57	105302.0
58	102790.0
59	92729.0
60	82668.0
61	72682.5
62	62697.0
63	54539.5
64	46382.0
65	39399.0
66	32416.0
67	27897.5
68	23379.0
69	21126.0
70	18873.0
71	14687.0
72	10501.0
73	8838.5
74	7176.0
75	5688.0
76	4200.0
77	3588.0
78	2976.0
79	2460.0
80	1944.0
81	1534.0
82	1124.0
83	967.0
84	810.0
85	586.0
86	362.0
87	260.5
88	159.0
89	118.0
90	77.0
91	71.5
92	66.0
93	53.0
94	40.0
95	41.0
96	42.0
97	28.5
98	15.0
99	13.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007599928977654864
2	3.362800432590648E-4
3	1.345120173036259E-4
4	0.0
5	0.0
6	3.362800432590648E-4
7	1.345120173036259E-4
8	0.0
9	9.415841211253813E-4
10	8.070721038217554E-4
11	0.0
12	0.0
13	4.7079206056269065E-4
14	1.345120173036259E-4
15	0.0044388965710196555
16	5.380480692145035E-4
17	0.0
18	3.362800432590648E-4
19	0.0
20	6.053040778663166E-4
21	0.0
22	0.0
23	2.690240346072518E-4
24	5.380480692145035E-4
25	0.002152192276858014
26	0.005649504726752288
27	0.005447736700796849
28	0.003833592493153338
29	0.003026520389331583
30	0.004035360519108778
31	0.007330904943047612
32	0.00295926438067977
33	0.004304384553716028
34	0.0020849362682062015
35	0.0032282884152870215
36	0.007599928977654864
37	0.0036318244671978994
38	0.007667184986306676
39	0.004035360519108778
40	0.0017486562249471368
41	0.0033628004325906478
42	0.0015468881989916979
43	0.001479632190339885
44	0.0012106081557326331
45	0.001412376181688072
46	0.0012778641643844462
47	0.0024212163114652662
48	0.0016141442076435108
49	4.035360519108777E-4
50	0.0016814002162953239
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1486856.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.53548000974355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25953853272514	50.003447089814934
2	11.863295846778852	14.600272088582678
3	3.2568637695748803	6.012380261613991
4	1.2809187605090133	3.1528780312563147
5	0.6485436220081292	1.995422154376396
6	0.36765319714857353	1.3574229578192611
7	0.2503597416066494	1.0784204812416376
8	0.17886583661971162	0.8805276090990655
9	0.1352998982389967	0.7493169765065507
>10	0.6358138267245744	7.078114029249669
>50	0.059553171696803076	2.606091048628501
>100	0.05613893877846167	7.176309918591568
>500	0.005833960804340534	2.355349669726508
>1k	0.001320896785888423	0.9540476834928785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.0176802595543887E-4
13	0.0	0.0	0.0	0.0	2.0176802595543887E-4
14	0.0	0.0	0.0	0.0	2.6902403460725183E-4
15	0.0	0.0	0.0	0.0	2.6902403460725183E-4
16	0.0	0.0	0.0	0.0	2.6902403460725183E-4
17	0.0	0.0	0.0	0.0	2.6902403460725183E-4
18	0.0	0.0	0.0	1.3451201730362591E-4	2.6902403460725183E-4
19	0.0	0.0	0.0	2.0176802595543887E-4	2.6902403460725183E-4
20	0.0	0.0	0.0	2.0176802595543887E-4	4.0353605191087774E-4
21	0.0	0.0	0.0	2.6902403460725183E-4	4.7079206056269065E-4
22	0.0	0.0	0.0	2.6902403460725183E-4	5.380480692145037E-4
23	0.0	0.0	0.0	6.725600865181296E-4	5.380480692145037E-4
24	0.0	0.0	0.0	8.743281124735684E-4	5.380480692145037E-4
25	0.0	0.0	0.0	0.0011433521470808202	5.380480692145037E-4
26	0.0	0.0	0.0	0.001412376181688072	5.380480692145037E-4
27	0.0	0.0	0.0	0.0018159122335989497	5.380480692145037E-4
28	0.0	0.0	0.0	0.0032955444239388346	5.380480692145037E-4
29	0.0	0.0	0.0	0.005447736700796849	5.380480692145037E-4
30	0.0	0.0	0.0	0.01163528949676364	5.380480692145037E-4
31	0.0	0.0	0.0	0.026969659469376996	5.380480692145037E-4
32	0.0	0.0	0.0	0.043649149615026604	5.380480692145037E-4
33	0.0	0.0	0.0	0.0612702238818016	5.380480692145037E-4
34	0.0	0.0	0.0	0.08319568270229262	5.380480692145037E-4
35	0.0	0.0	0.0	0.10787863787750798	5.380480692145037E-4
36	0.0	0.0	0.0	0.14083408211689633	5.380480692145037E-4
37	0.0	0.0	0.0	0.18972920040676433	5.380480692145037E-4
38	0.0	0.0	0.0	0.25974270541330163	5.380480692145037E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2625	0.0	20.450445	1
CCGTTAT	60	0.0074080806	18.335392	31
CGAATTT	60	0.007409518	18.334778	28
GTATTAA	230	0.0	18.174732	1
CGTCGTA	415	0.0	18.022486	10
ATACCGT	455	0.0	17.404451	6
CGAGCCG	510	0.0	17.253939	15
TCTATAC	90	2.2178865E-4	17.109007	3
TAGGACG	340	0.0	16.821459	4
TACCGTC	445	0.0	16.80692	7
ATCCCGA	105	3.7781785E-5	16.760406	16
CGCAATA	390	0.0	16.360813	36
ACCGTCG	460	0.0	16.258867	8
TAGGACA	385	0.0	15.998031	4
AACGCAG	3345	0.0	15.980097	6
CCGTCGT	485	0.0	15.874868	9
GGTATCA	1485	0.0	15.852567	1
GGTATAT	125	9.439069E-6	15.840714	1
GTCGTAG	460	0.0	15.781197	11
TAGCGGC	405	0.0	15.754327	30
>>END_MODULE